Abstract
Objectives
To select aptamers for endotoxin separation from a 75-nucleotide single-stranded DNA random library using systematic evolution of ligands by exponential enrichment.
Results
After 15 rounds of selection, the final pool of aptamers was specific to endotoxin. Structural analysis of aptamers that appeared more than once suggested that one aptamer can form a G-quartet structure. Tests for binding affinity and specificity showed that this aptamer exhibited a high affinity for endotoxin. Using this aptamer, aptamer-magnetic beads were designed to separate endotoxin.
Conclusions
Using these aptamer-magnetic beads, a new method to separate endotoxin was developed to enable specific separation of endotoxin that can be applied to drug and food products.
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Ying, G., Zhu, F., Yi, Y. et al. Selecting DNA aptamers for endotoxin separation. Biotechnol Lett 37, 1601–1605 (2015). https://doi.org/10.1007/s10529-015-1839-8
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DOI: https://doi.org/10.1007/s10529-015-1839-8