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Gene chip analysis of Arabidopsis thaliana genomic DNA methylation and gene expression in response to carbendazim

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Abstract

Objectives

To examine the effects of carbendazim on Arabidopsis genomic DNA methylation and gene expression.

Results

Carbendazim caused widespread changes in gene loci methylation and gene expression. With 0.1 mM (D2) and 0.2 mM (D3) carbendazim, there were, respectively, 1522 and 2278 demethylated sites and 1541 and 2790 methylated sites. A total of 279 and 505 genes were up-regulated by more than 300 % and 175 and 609 genes were down-regulated by 67 % in D2 and D3 treatments, respectively, compared with the control. Conjoint analysis showed that 20 and 39 demethylated genes were up-regulated >300 % and 21 and 24 methylated genes were down-regulated <67 % in D2 and D3, respectively.

Conclusions

Carbendazim causes methylation or demethylation of certain genes and changes the expression of these genes. These findings provide a theoretical basis for novel epigenetics-based methods to detect organic food and a new interpretation for the degradation of crop varieties.

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Acknowledgments

Financial support from the National Science Foundation of China(no. 31171982, no. 31372090)and the Foundation of Henan University (China) (no. 0000A40448).

Conflict of interests

The authors declare that they have no conflict of interests.

Supporting information

Supplementary Fig. 1—The length of roots was significantly decreased with the carbendazim added.

Supplementary Fig. 2—The number of fibrous roots was significantly increased with the carbendazim added.

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Author notes

    Authors and Affiliations

    1. Plant Germplasm Resources and Genetic Laboratory, College of Life Science, Henan University, Jinming Road, Kaifeng, 475004, Henan, China

      Zhongai Li, Zicheng Wang & Suoping Li

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    1. Zhongai Li
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    2. Zicheng Wang
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    3. Suoping Li
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    Correspondence to Suoping Li.

    Additional information

    Zhongai Li and Zicheng Wang have contributed equally.

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    Li, Z., Wang, Z. & Li, S. Gene chip analysis of Arabidopsis thaliana genomic DNA methylation and gene expression in response to carbendazim. Biotechnol Lett 37, 1297–1307 (2015). https://doi.org/10.1007/s10529-015-1789-1

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    • DOI: https://doi.org/10.1007/s10529-015-1789-1

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