Abstract
Tremella fuciformis yeast-like conidium (YLC) cells were transformed by co-cultivation with Agrobacterium cells harboring the hepatitis B surface antigen (HBsAg) gene construct under the control of the CaMV35S promoter. Integration of HBsAg DNA into the YLC genome was confirmed by PCR and dot-blot hybridization. Immunoblotting verified expression of the recombinant protein. Oral administration of YLC cells expressing HBsAg in mice significantly increased anti-HBsAg antibody titer levels using a double prime-boost strategy that combined parenteral and oral HBsAg boosters.
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This work was supported by the Research Funds for 2011 from the Catholic University of Daegu.
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Shin, DI., Song, KS. & Park, HS. Oral vaccination of mice with Tremella fuciformis yeast-like conidium cells expressing HBsAg. Biotechnol Lett 37, 539–544 (2015). https://doi.org/10.1007/s10529-014-1720-1
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DOI: https://doi.org/10.1007/s10529-014-1720-1