Skip to main content
SpringerLink
Log in

miR-31 promotes proliferation of colon cancer cells by targeting E2F2

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

MicroRNA-31 (miR-31) plays important roles in colon cancer development. However, the underlying mechanism is still not clear. We have explored the functions of miR-31 on proliferation of colon cancer cells as well as the underlying mechanism. E2F2 was identified as a direct target of miR-31. miR-31 regulated the proliferation of colon cancer cells by targeting E2F2. Moreover, in the present study, E2F2 acted as a tumor suppressor in colon cancer by repressing the expression of survivin and regulating the expression of CCNA2, C-MYC, MCM4 and CDK2. A possible mechanism for the function of miR-31 on colon cancer proliferation is presented and indicates that miR-31 might become a target for anti-cancer drug design.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Azkargorta M, Fullaondo A, Laresgoiti U, Aloria K, Infante A, Arizmendi JM, Zubiaga AM (2010) Differential proteomics analysis reveals a role for E2F2 in the regulation of the Ahr pathway in T lymphocytes. Mol Cell Proteomics 9:2184–2194

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Carrington JC, Ambros V (2003) Role of microRNAs in plant and animal development. Science 301:336–338

    Article  CAS  PubMed  Google Scholar 

  • Cekaite L et al (2012) MiR-9, -31, and -182 deregulation promote proliferation and tumor cell survival in colon cancer. Neoplasia 14:868–879

    PubMed Central  CAS  PubMed  Google Scholar 

  • Creighton CJ et al (2010) Molecular profiling uncovers a p53-associated role for microRNA-31 in inhibiting the proliferation of serous ovarian carcinomas and other cancers. Cancer Res 70:1906–1915

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Dong Q et al (2010) MicroRNA let-7a inhibits proliferation of human prostate cancer cells in vitro and in vivo by targeting E2F2 and CCND2. PLoS ONE 5:e10147

    Article  PubMed Central  PubMed  Google Scholar 

  • Dong Z, Zhong Z, Yang L, Wang S, Gong Z (2014) MicroRNA-31 inhibits cisplatin-induced apoptosis in non-small cell lung cancer cells by regulating the drug transporter ABCB9. Cancer Lett 343:249–257

    Article  CAS  PubMed  Google Scholar 

  • Infante A et al (2008) E2F2 represses cell cycle regulators to maintain quiescence. Cell Cycle 7:3915–3927

    Article  CAS  PubMed  Google Scholar 

  • Johnson DG, Degregori J (2006) Putting the oncogenic and tumor suppressive activities of E2F into context. Curr Mol Med 6:731–738

    CAS  PubMed  Google Scholar 

  • Lal A et al (2009) miR-24 Inhibits cell proliferation by targeting E2F2, MYC, and other cell-cycle genes via binding to “seedless” 3′UTR microRNA recognition elements. Mol Cell 35:610–625

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Laurila EM, Kallioniemi A (2013) The diverse role of miR-31 in regulating cancer associated phenotypes. Genes Chromosom Cancer 52:1103–1113

    Article  CAS  PubMed  Google Scholar 

  • Li T, Yang J, Lv X, Liu K, Gao C, Xing Y, Xi T (2014) miR-155 regulates the proliferation and cell cycle of colorectal carcinoma cells by targeting E2F2. Biotechnol Lett 36:1743–1752

    Article  CAS  PubMed  Google Scholar 

  • Luo X, Burwinkel B, Tao S, Brenner H (2011) MicroRNA signatures: novel biomarker for colorectal cancer? Cancer Epidemiol Biomark Prev 20:1272–1286

    Article  CAS  Google Scholar 

  • Opavsky R et al (2007) Specific tumor suppressor function for E2F2 in Myc-induced T cell lymphomagenesis. Proc Natl Acad Sci USA 104:15400–15405

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Pusapati RV, Weaks RL, Rounbehler RJ, McArthur MJ, Johnson DG (2010) E2F2 suppresses Myc-induced proliferation and tumorigenesis. Mol Carcinog 49:152–156

    PubMed Central  CAS  PubMed  Google Scholar 

  • Valastyan S, Weinberg RA (2010) miR-31: a crucial overseer of tumor metastasis and other emerging roles. Cell Cycle 9:2124–2129

    Article  CAS  PubMed  Google Scholar 

  • Wu N et al (2012) miR-125b regulates the proliferation of glioblastoma stem cells by targeting E2F2. FEBS Lett 586:3831–3839

    Article  CAS  PubMed  Google Scholar 

  • Xanthoulis A, Tiniakos DG (2013) E2F transcription factors and digestive system malignancies: how much do we know? World J Gastroenterol 19:3189–3198

    Article  PubMed Central  PubMed  Google Scholar 

  • Xi S et al (2010) Cigarette smoke induces C/EBP-beta-mediated activation of miR-31 in normal human respiratory epithelia and lung cancer cells. PLoS ONE 5:e13764

    Article  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported by Grants from National Natural Science Foundation of China (No. 81372331) and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

Supporting information

Supplementary Table 1 - Primers for quantitative real-time PCR.

Supplementary Table 2 - Primers for plasmids construct.

Author information

Authors and Affiliations

  1. School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, People’s Republic of China

    Tong Li, Wenjing Luo, Xiaobo Lv & Tao Xi

  2. Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, Nanjing, 210009, People’s Republic of China

    Tong Li, Wenjing Luo, Xiaobo Lv & Tao Xi

  3. Ningxia Key Laboratory of Cerebrocranial Diseases, Ningxia Medical University, Yinchuan, 750004, People’s Republic of China

    Kunmei Liu

Authors
  1. Tong Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wenjing Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kunmei Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiaobo Lv
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tao Xi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tao Xi.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 13 kb)

Supplementary material 2 (DOCX 13 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Li, T., Luo, W., Liu, K. et al. miR-31 promotes proliferation of colon cancer cells by targeting E2F2. Biotechnol Lett 37, 523–532 (2015). https://doi.org/10.1007/s10529-014-1715-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-014-1715-y

Keywords

Search

Navigation