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Maintenance of human adipose derived stem cell (hASC) differentiation capabilities using a 3D culture

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Abstract

In this study, 3D culture system for human adipose-derived stem cell (hASC) using a BioLevitator as the bioreactor for microcarrier-based cultures was established. During the culturing period, hASCs preferred to grow in crevices between microcarriers and a high viability was maintained even when reaching confluency. Adipogenic or osteogenic differential medium was used to induce hASCs and differential potentials of these cells were compared between 2D and 3D environments via RT-PCR and staining quantifications. CEBP/α gene expression was significant higher in 3D condition at day 21 (P < 0.05). Staining quantification indicates that cells cultured in 3D condition have significant better differentiation potential from day 14 to 21 for both adipogenic and osteogenic lineages (P < 0.01).

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Acknowledgments

We would like to thank the National Science Council of Taiwan for financially supporting this research under contract No. NSC 102-2221-E-002-039, and Dr. Nai-Chen Cheng from Department of Surgery, National Taiwan University for providing adipose tissue obtained from patients undergoing liposuction procedures. The study protocol has been approved by the Internal Ethical Committee of the National Taiwan University Hospital.

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Authors and Affiliations

  1. Department of Chemical Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan

    Ching-Yu Lin, Chi-Hui Huang & Jiashing Yu

  2. College of Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan

    Yuan-Kun Wu

  3. Department of Surgery, National Taiwan University Hospital, No.1, Changde St., Zhongzheng Dist., Taipei, 10048, Taiwan

    Nai-Chen Cheng

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  1. Ching-Yu Lin
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  2. Chi-Hui Huang
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  3. Yuan-Kun Wu
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  4. Nai-Chen Cheng
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  5. Jiashing Yu
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Corresponding author

Correspondence to Jiashing Yu.

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Lin, CY., Huang, CH., Wu, YK. et al. Maintenance of human adipose derived stem cell (hASC) differentiation capabilities using a 3D culture. Biotechnol Lett 36, 1529–1537 (2014). https://doi.org/10.1007/s10529-014-1500-y

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  • DOI: https://doi.org/10.1007/s10529-014-1500-y

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