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A new recombinant hybrid polypeptide and its immunologic adjuvant activity for inactivated infectious bursal disease vaccine

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Abstract

Both bursin (Lys–His–Gly–NH2) and Gagnon’s peptides (Lys–Asn–Pro–Tyr) can induce B-cell differentiation. However, it is unclear whether a recombinant hybrid polypeptide consisting of a tandem array of 14 copies of bursin and two copies of Gagnon’s peptide can induce the proliferative activity of lymphocytes. Here, this recombinant hybrid polypeptide was expressed in Escherichia coli and purified by SDS-PAGE. Various assays showed that it not only promoted B-lymphocyte proliferation in vitro but also increased the titers of antibodies directed against infectious bursal disease virus fourfold in the sera of chickens vaccinated with the inactivated infectious bursal disease virus vaccine. The recombinant hybrid polypeptide also reduced the pathological lesions in the bursa of Fabricius caused by infectious bursal disease virus BC6/85. Our results show that this recombinant hybrid polypeptide may be a promising immune adjuvant.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China, Science Fund for Creative Research Groups (No. 81121062), and the National Natural Science Foundation of China (No. 81001665), Jiangsu Planned Projects for Postdoctoral Research Funds (No. 1202042C), and Jiangsu University Funds (No. 12JDG099). We would like to thank Prof. Wang Yong and Dr. John Owusu for providing language support.

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Authors and Affiliations

  1. School of Food and Biology Engineering, Jiangsu University, Zhenjiang, 212013, Jiangsu, China

    Mei-hong Cai

  2. State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, 210093, Jiangsu, China

    Mei-hong Cai, Feng Zhu, Hao-chen Wu & Ping-ping Shen

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  1. Mei-hong Cai
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  2. Feng Zhu
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  3. Hao-chen Wu
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  4. Ping-ping Shen
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Correspondence to Ping-ping Shen.

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Cai, Mh., Zhu, F., Wu, Hc. et al. A new recombinant hybrid polypeptide and its immunologic adjuvant activity for inactivated infectious bursal disease vaccine. Biotechnol Lett 36, 1431–1437 (2014). https://doi.org/10.1007/s10529-014-1499-0

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  • DOI: https://doi.org/10.1007/s10529-014-1499-0

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