Abstract
The keratinase Sfp2, produced by Streptomyces fradiae var. k11, is a serine alkaline protease first synthesized as pre-pro-mature precursor, of which the N-terminal propeptide must be autocatalytically cleaved on the C-terminal of P1 amino acid to produce mature enzyme. Single amino acid substitutions were introduced at positions −1 and −2 to improve the expression level of mature Sfp2. The specific activity of L(−1)F mutant (48935 U/mg) was nine times that of wild-type Sfp2, whereas the mutants L(−1)D, L(−1)G, L(−1)H, K(−2)E, and K(−2)L had 2–52 % of the specific activity of wild-type. The yield of mature Sfp2 of L(−1)F mutant was estimated to be 800 μg/mg total protein and 112 mg/l culture supernatant, nine and twice that of wild-type, respectively. The L(−1)F mutant exhibited similar enzymatic properties to wild-type.
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Acknowledgments
We thank Dr. Xi Huang at Shanghai Jiao Tong University for his valuable comments. This research was supported by the ‘Five-twelfth’ National Science and Technology Support Program of China (2011BAD26B0403).
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Li, J., Chen, D., Yu, Z. et al. Improvement of expression level of keratinase Sfp2 from Streptomyces fradiae by site-directed mutagenesis of its N-terminal pro-sequence. Biotechnol Lett 35, 743–749 (2013). https://doi.org/10.1007/s10529-013-1139-0
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DOI: https://doi.org/10.1007/s10529-013-1139-0