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Cytosolic protection against ultraviolet induced DNA damage by blueberry anthocyanins and anthocyanidins in hepatocarcinoma HepG2 cells

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Abstract

UV-induced DNA damage plays a key role in the etiology of certain diseases. The ability of blueberry anthocyanins and anthocyanidins (BA) to protect cellular DNA from UV-induced damage was investigated. BA were extracted by water (BAW), ethanol (BAE) or methanol (BAM). These extracts partially restored proliferation of UV-irradiated HepG2 cells as shown by MTT assay. Treatment with BA extracts at 75 μg/ml decreased reactive oxygen species and decreased DNA damage by tail moment of comet assay and expression of γH2AX in situ. BAM significantly decreased gene and protein expression of p53, phospho-p53 (Ser15), and p21 in UV-irradiated HepG2 cells. BA thus efficiently protects cells from DNA damage in vitro. Blueberry may potentially be used as a good source of naturally radioprotective agents.

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Acknowledgments

This project was supported by an initial fund from Tianjin city government for “1000 talents plan” program to C. L. Authors are thankful to Dr. Lei Zhang, Hualida Co. Tianjin, China for his critical comments for preparation of the manuscripts.

Conflict of interest

The authors declare no conflicts of interest.

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Authors and Affiliations

  1. Key Laboratory of Food Nutrition and Safety, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, No. 29, The 13th Avenue, TEDA, Tianjin, 300457, China

    Wei Liu, Xiangyi Lu, Xiang Gao, Mengxian Li, Junhua Wu, Zhenjing Li & Cheng Luo

  2. School of Life Science, Xiamen University, Xiamen, 361005, China

    Guangyang He

  3. School of Life Science, Chinese University of Science and Technology, Hefei, 230026, China

    Jihui Wu & Juncheng Wang

Authors
  1. Wei Liu
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  2. Xiangyi Lu
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  3. Guangyang He
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  4. Xiang Gao
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  5. Mengxian Li
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  6. Junhua Wu
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  7. Zhenjing Li
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  8. Jihui Wu
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  9. Juncheng Wang
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  10. Cheng Luo
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Corresponding author

Correspondence to Cheng Luo.

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Liu, W., Lu, X., He, G. et al. Cytosolic protection against ultraviolet induced DNA damage by blueberry anthocyanins and anthocyanidins in hepatocarcinoma HepG2 cells. Biotechnol Lett 35, 491–498 (2013). https://doi.org/10.1007/s10529-012-1105-2

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  • DOI: https://doi.org/10.1007/s10529-012-1105-2

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