Abstract
Adding a cationic polyacrylamide (c-PAM) to either the amylase mediated hydrolysis of corn starch or the hydrolysis of wood fiber by cellulase can enhance the initial hydrolysis rates, although a rate decrease can occur under some conditions. Several c-PAMs can serve as catalysts and the same c-PAM can improve the efficiency of both amylase and cellulase. The initial amylase rate approximately doubles; the analogous cellulase hydrolysis rate increases by about 40%. c-PAMs increase the binding of enzyme to substrate.
Similar content being viewed by others
References
Arica MY, Yavuz H, Patir S et al (2000) Immobilization of glucoamylase onto spacer-arm attached magnetic polymethylmethacrylate/microspheres: characterization and application to a continuous flow reactor. J Mol Catal B 11:127–138
Banerjee S, Reye JT (2008) Means for enhancing rates of enzymatic processes. US and international patents pending
Bolto B, Gregory J (2007) Organic polyelectrolytes in water treatment. Water Res 41:2301–2324
Hartley WH, Banerjee S (2008) Imaging c-PAM-induced flocculation of paper fibers. J Colloid Interface Sci 320:159–162
Kwiatkowski R, McAloon AJ, Taylor F et al (2006) Modeling the process and costs of fuel ethanol production by the corn dry-grind process. Ind Crop Prod 23:288–296
Perez J, Munoz-Dorado J, de la Rubia T et al (2002) Biodegradation and biological treatments of cellulose, hemicellulose and lignin: an overview. Int Microbiol 5:53–63
TAPPI (2000) Forming handsheets for physical tests of pulp, test method T 205 sp-06. Tappi Press, Atlanta, GA
Wang Y, Hsieh Y-L (2004) Enzyme immobilization to ultra-fine cellulose fibers via amphiphilic polyethylene glycol spacers. J Polym Sci A 42:4289–4299
Yoon S-Y, Deng Y (2004) Flocculation and reflocculation of clay suspension by different polymer systems under turbulent conditions. J Colloid Interface Sci 278:139–145
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Reye, J.T., Maxwell, K., Rao, S. et al. Cationic polyacrylamides enhance rates of starch and cellulose saccharification. Biotechnol Lett 31, 1613–1616 (2009). https://doi.org/10.1007/s10529-009-0053-y
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-009-0053-y