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Association of Pro12Ala Polymorphism of Peroxisome Proliferator-Activated Receptor gamma 2 (PPARγ2) Gene with Type 2 Diabetes Mellitus in Ethnic Kashmiri Population

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ABSTRACT

Type 2 diabetes mellitus (T2DM) is characterized by chronic hyperglycemia associated with insulin resistance and relative insulin deficiency. T2DM is believed to be attributable to the combined effect of genetic and environmental factors. Peroxisome proliferator-activated receptor gamma 2 (PPARγ2) is one of the main candidate genes that are implicated in T2DM. A common proline 12 alanine (Pro12Ala) polymorphism in PPARγ2 has been shown to be associated with T2DM. The aim of this work was to investigate the possible role of PPARγ2 gene polymorphism, as a genetic risk factor for T2DM. The study comprised 200 ethnic unrelated subjects (100 T2DM patients and 100 controls). PCR–RFLP technique was used for genotyping analysis. The frequency of the Pro allele was 79 and 91.5 % for controls and cases, respectively (P < 0.05; OR 3.2; 95 % CI 1.64–6.3). The Pro12Ala polymorphism was in Hardy–Weinberg equilibrium in both patients and controls (χ 2 = 0.13, P > 0.05). We found a significant association of Pro12Ala polymorphism of PPARγ2 gene with T2DM, however the genotypes showed statistically significant association only with few clinical parameters including body mass index, total cholesterol, and low-density lipoprotein (P < 0.05). The study signifies that Pro allele in PPARγ2 may be a genotypic risk factor that confers susceptibility to T2DM in ethnic Kashmiri population.

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Acknowledgments

We thank all of the subjects who voluntarily participated in the study. We gratefully acknowledge the assistance of the nursing staff and consultants at SMHS Hospital for their help during sample collection. We also thank the Department of Biochemistry, University of Kashmir Srinagar J&K for funding this study and providing the lab facilities.

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Authors and Affiliations

  1. Department of Biochemistry, University of Kashmir, Srinagar, India

    Misbah Majid, Akbar Masood & Iqra Hameed

  2. Department of Gastroenterology, Government Medical College and Hospital, Srinagar, India

    Showkat Ahmad Kadla

  3. Centre for Research and Development, University of Kashmir, Srinagar, India

    Bashir A. Ganai

Authors
  1. Misbah Majid
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  2. Akbar Masood
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  3. Showkat Ahmad Kadla
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  4. Iqra Hameed
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  5. Bashir A. Ganai
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Corresponding author

Correspondence to Bashir A. Ganai.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Animal Research Concern

This article does not contain any studies with animals performed by any of the authors.

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Informed consent was obtained from all individual participants included in the study.

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Majid, M., Masood, A., Kadla, S.A. et al. Association of Pro12Ala Polymorphism of Peroxisome Proliferator-Activated Receptor gamma 2 (PPARγ2) Gene with Type 2 Diabetes Mellitus in Ethnic Kashmiri Population. Biochem Genet 55, 10–21 (2017). https://doi.org/10.1007/s10528-016-9765-6

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  • DOI: https://doi.org/10.1007/s10528-016-9765-6

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