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Bacterial Overexpression of Recombinant Heteroscorpine-1 (rHS-1), a Toxin from Heterometrus laoticus Scorpion Venom: Trends for Antibacterial Application and Antivenom Production

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Abstract

Heteroscorpine-1 (HS-1) was identified as a member of the scorpine family. HS-1 shows insecticidal activities, exhibiting a low median lethal dose (LD50) in mealworm (Tenebrio molitor L.) and inhibitory activities against Bacillus subtilis, Klebsiella pneumoniae, and Pseudomonas aeruginosa. In this study, a recombinant HS-1 (rHS-1) was produced by overexpression in E. coli. A large yield of product was obtained. The structure of purified rHS-1 was confirmed through mass spectrometry. Both anti-crude venom and anti-rHS-1 antibodies specifically recognized rHS-1, suggesting its structural similarity. Reactivated rHS-1 caused roughening and blebbing of bacterial cell surfaces. It showed higher activity than that of pre-refolded protein. Antisera raised against a partially purified and mis- or unfolded peptide can inhibit relevant bioactivity.

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Acknowledgments

This work was supported by the Japan Society for the Promotion of Science (JSPS) and the TRF-CHE Research Grant for Mid-Career University Faculty; jointly funded by the Thailand Research Fund (TRF) and the Office of the Higher Education Commission (CHE), Ministry of Education, Thailand; and additionally supported by the Khon Kaen University (KKU) Research Fund, fiscal years 2006–2010. This research was also supported by a grant from the Strategic Scholarships for Frontier Research Network for the Thai Postdoctoral Programs to N.U. from CHE and Synchrotron Light Research Institute (SLRI), Thailand, grant number 2552/07. We would like to thank Prof. Yukifumi Nawa of the KKU Publication Clinic for critical reading of the manuscript.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Khon Kaen University, Khon Kaen, 40002, Thailand

    Nunthawun Uawonggul, Sophida Sukprasert, Paroonkorn Incamnoi, Rina Patramanon, Sompong Thammasirirak, Sutthidech Preecharram & Sakda Daduang

  2. Chulabhorn International College of Medicine, Thammasat University, Rangsit Center, Pathumthani, 12121, Thailand

    Sophida Sukprasert

  3. Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

    Wandee Bunyatratchata

  4. Synchrotron Light Research Institute (Public Organization), 111 University Avenue, Muang District, Nakhon Ratchasima, 30000, Thailand

    Buabarn Kuaprasert

  5. Department of Clinical Chemistry, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand

    Jureerut Daduang

  6. Department of Chemistry, Faculty of Engineering, Rajamangala University of Technology Isan, Khon Kaen Campus, Khon Kaen, 40000, Thailand

    Paroonkorn Incamnoi

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  1. Nunthawun Uawonggul
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  2. Sophida Sukprasert
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  3. Paroonkorn Incamnoi
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  4. Rina Patramanon
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  5. Sompong Thammasirirak
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  6. Sutthidech Preecharram
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  7. Wandee Bunyatratchata
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  8. Buabarn Kuaprasert
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  9. Jureerut Daduang
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  10. Sakda Daduang
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Corresponding author

Correspondence to Sakda Daduang.

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Uawonggul, N., Sukprasert, S., Incamnoi, P. et al. Bacterial Overexpression of Recombinant Heteroscorpine-1 (rHS-1), a Toxin from Heterometrus laoticus Scorpion Venom: Trends for Antibacterial Application and Antivenom Production. Biochem Genet 52, 459–473 (2014). https://doi.org/10.1007/s10528-014-9660-y

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  • DOI: https://doi.org/10.1007/s10528-014-9660-y

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