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Novel bacterial endophytes from Hevea brasiliensis as biocontrol agent against Phytophthora leaf fall disease

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Abstract

Bacterial endophytes offer control against many diseases of crop plants as potential biocontrol agents. Antagonistic bacterial endophytes acting against Phytophthora meadii have been screened from leaf, petiole and root tissues of Hevea brasiliensis. Six bacterial endophytes could exhibit more than 50 % inhibition of P. meadii, among which EIL-2, from disease-free zones showed a maximum of 62.5 % inhibition. The isolate EIL-2 was characterized as Alcaligenes sp. and the other isolates were identified as Pseudomonas aeruginosa. 16S rDNA sequence analysis showed that there existed genetic variation among the five isolates of P. aeruginosa from different tissues of the plant indicating the tissue type adaptation of the isolates. Dual culture technique with endophyte EIL-2 completely arrested the growth of P. meadii when inoculated prior to pathogen. The bioassay with EIL-2 in H. brasiliensis clones, RRII 105 showed 43 % reduction of lesion size on infected leaves whereas in RRIM 600 it was only 30 %.

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Acknowledgments

The authors are highly thankful for the facilities provided at Rubber Research Institute of India and Mahatma Gandhi University, Kottayam, India.

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Authors and Affiliations

  1. Rubber Research Institute of India, Kottayam, Kerala, India

    Amith Abraham, Shaji Philip & C. Kuruvilla Jacob

  2. School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India

    K. Jayachandran

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  1. Amith Abraham
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  2. Shaji Philip
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  3. C. Kuruvilla Jacob
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  4. K. Jayachandran
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Correspondence to K. Jayachandran.

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Abraham, A., Philip, S., Kuruvilla Jacob, C. et al. Novel bacterial endophytes from Hevea brasiliensis as biocontrol agent against Phytophthora leaf fall disease. BioControl 58, 675–684 (2013). https://doi.org/10.1007/s10526-013-9516-0

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  • DOI: https://doi.org/10.1007/s10526-013-9516-0

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