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Mycovirus effect on the endophytic establishment of the entomopathogenic fungus Tolypocladium cylindrosporum in tomato and bean plants

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Abstract

Two endophytic strains of the entomopathogenic fungus Tolypocladium cylindrosporum, originally isolated from the grass Festuca rubra, were artificially inoculated in tomato and bean plants. Strains 11-1L and 11-0BR were isolated from asymptomatic leaf fragments of both plant species at 3, 7, 14, 21, and 35 days after their inoculation. The percentage of leaf fragments infected by the fungus in inoculated leaves decreased at each sampling time, and no systemic colonization of the plants occurred. The two T. cylindrosporum strains tested were isogenic, differing in the infection by the victorivirus TcV1, harboured by strain 11-1L, but not by 11-0BR. The percentage of infected leaf fragments in leaves inoculated with the virus infected strain was greater in bean than in tomato plants, while the virus-free strain was more successful in tomato than in bean plants. This result suggests that the mycovirus infection can affect the adaptation of T. cylindrosporum to particular host plants.

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Acknowledgments

This work was financed with research grant AGL2008-01159, awarded by the Spanish Government.

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  1. Noemí Herrero Asensio

    Present address: Fondazione Edmund Mach, IASMA Research and Innovation Centre, Via E. Mach 1, 38010, S. Michele all’Adige, TN, Italy

Authors and Affiliations

  1. Department of Abiotic Stress, Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), Cordel de Merinas 40-52, 37008, Salamanca, Spain

    Noemí Herrero Asensio, Salud Sánchez Márquez & Iñigo Zabalgogeazcoa

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  1. Noemí Herrero Asensio
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  2. Salud Sánchez Márquez
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  3. Iñigo Zabalgogeazcoa
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Correspondence to Iñigo Zabalgogeazcoa.

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Handling Editor: Helen Roy

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Herrero Asensio, N., Sánchez Márquez, S. & Zabalgogeazcoa, I. Mycovirus effect on the endophytic establishment of the entomopathogenic fungus Tolypocladium cylindrosporum in tomato and bean plants. BioControl 58, 225–232 (2013). https://doi.org/10.1007/s10526-012-9476-9

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  • DOI: https://doi.org/10.1007/s10526-012-9476-9

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