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The effect of mitochondrial complex I inhibitor on longevity of short-lived and long-lived seed beetles and its mitonuclear hybrids

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Abstract

Mitochondria are suggested to play a central role in ageing and evolution of longevity. Gradual decline in mitochondrial function during ageing and concomitant increase in production of reactive oxygen species (ROS) leads to oxidative damage of macromolecules and impairment of ATP synthesis. To assess relationship between ageing and oxidative stress resistance we exposed different longevity lines of the seed beetle (Acanthoscelides obtectus) to four concentrations of tebufenpyrad, mitochondrial complex I inhibitor. Complex I is one of main sites of ROS production during normal respiration and its inhibition elevates oxidative stress. Our results showed that 24 h of exposure to tebufenpyrad decreased survival and post-stress longevity due to increased baseline mortality. Higher resistance was recorded in beetles from lines selected for late reproduction and extended longevity (L) than in early reproducing beetles (E). Also, females were more resistant than males. Since complex I is under dual genetic control, our second aim was to disentangle relative contribution of nuclear and mitochondrial genes to the variation in longevity. We used crossed combinations of distinct mitochondrial and nuclear genotypes (E × L, L × E) and compared them to control hybrids where mitochondrial genome was “transplanted” onto the original background (E × E, L × L). Our study revealed significant effect of nucleus, i.e. higher survival and post-stress longevity in beetles harbouring L nucleus. Mitochondrion effect was significant only within L nuclear background where E mitochondrion gave advantage.

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Acknowledgements

We thank the Editor and an anonymous referee for constructive comments and helpful suggestions. This work was supported by Ministry of Education, Science and Technological Development, Grant No. 173007.

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  1. Department of Evolutionary Biology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Despota Stefana Blvd 142, Belgrade, 11060, Serbia

    Darka Šešlija Jovanović, Mirko Đorđević & Uroš Savković

  2. Department of Insect Physiology and Biochemistry, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Despota Stefana Blvd 142, Belgrade, 11060, Serbia

    Jelica Lazarević

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  1. Darka Šešlija Jovanović
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  2. Mirko Đorđević
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Correspondence to Darka Šešlija Jovanović.

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Jovanović, D.Š., Đorđević, M., Savković, U. et al. The effect of mitochondrial complex I inhibitor on longevity of short-lived and long-lived seed beetles and its mitonuclear hybrids. Biogerontology 15, 487–501 (2014). https://doi.org/10.1007/s10522-014-9520-5

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