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Purification of High-Molecular-Weight Subfraction from Porcine Skin Inhibiting Proliferation of A431 Human Carcinoma Epidermoid Cells

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Bulletin of Experimental Biology and Medicine Aims and scope

Subfraction with a molecular weight >250 kDa isolated from porcine skin and inhibiting the proliferation of A431 human carcinoma epidermoid cells was purifi ed by DEAE 32 anion exchange chromatography with NaCl concentration step-gradient. The effects of the initial subfraction and fractions obtained by separation in DEAE 32 on the proliferation of A431 human carcinoma epidermoid cells were studied in vitro in two tests (MTT and fl uorescent test). The more sensitive fl uorescent test showed the highest inhibitory activity of fraction No. 2 released from the column at 0.15 M NaCl. One major protein component and a series of minor protein components were detected in this fraction by vertical PAAG-SDS electrophoresis.

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Authors and Affiliations

  1. Research Institute of Physico-Chemical Medicine, Federal Biomedical Agency of Russia, Moscow, Russia

    O. V. Belova, V. I. Sergienko, V. Ya. Arion, T. A. Lukanidina, S. N. Moskvina, I. V. Zimina, G. G. Borisenko, M. V. Grechikhina, E. V. Kovaleva & Zh. I. Klyuchnikova

  2. M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia

    G. V. Lutsenko & M. V. Grechikhina

Authors
  1. O. V. Belova
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  2. V. I. Sergienko
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  3. V. Ya. Arion
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  4. T. A. Lukanidina
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  5. S. N. Moskvina
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  6. I. V. Zimina
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  7. G. G. Borisenko
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  8. G. V. Lutsenko
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  9. M. V. Grechikhina
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  10. E. V. Kovaleva
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  11. Zh. I. Klyuchnikova
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Corresponding author

Correspondence to O. V. Belova.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 157, No. 3, pp. 352-356, March, 2014

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Belova, O.V., Sergienko, V.I., Arion, V.Y. et al. Purification of High-Molecular-Weight Subfraction from Porcine Skin Inhibiting Proliferation of A431 Human Carcinoma Epidermoid Cells. Bull Exp Biol Med 157, 371–374 (2014). https://doi.org/10.1007/s10517-014-2569-y

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  • DOI: https://doi.org/10.1007/s10517-014-2569-y

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