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Lipidomic profiling of juvenile yellow head catfish (Pelteobagrus fulvidraco) in response to Fucoidan diet

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Abstract

Yellow head catfish, Pelteobagrus fulvidraco, with delicious meat and high commercial value has relatively high lipid accumulation which is considered to be a major problem in aquaculture. It was found that fucoidan has the functions in reducing lipid concentration and enhancing the immunity capability. The purpose of this study is to understand the role of fucoidan in juvenile yellow head catfish and the underlying comprehensive lipid metabolic regulation. The overall changes of significant lipid biomarkers in juvenile yellow head catfish fed on 0.1% fucoidan at different time points were studied by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-ESI-Q-TOF-MS) and multivariate statistical analysis. At the eighth week, the lipid profile had the largest difference between the two groups, with 10 lipids identified as potential lipid biomarkers. Lysophosphatidylcholine (Lyso-PC), phosphatidylcholine (PC), and phosphatidylethanolamine (PE) were elevated, while triglycerides (TAGs) were reduced in SF group compared to C group. Overall, the content of free fatty acids (FFAs), derived from TAG, was decreased, whereas that of total fatty acids (TFAs) derived from FFAs showed the opposite tendency. Fucoidan can reduce the content of triglyceride perhaps by altering the activities of related enzymes and improve immunity capability of the juvenile yellow head catfish through increasing the whole levels of related fatty acids, which may be useful for facilitating efficient development and scientific improvement of fishery.

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Abbreviations

UPLC:

Ultra-performance liquid chromatography

Q-TOF-MS:

Quadrupole time-of-flight mass spectrometry

MS/MS:

Tandem mass spectrometry

TIC:

Total ion current chromatogram

EIC:

Extracted ion chromatogram

ESI:

Electrospray ionization

RT:

Retention time

GC-MS:

Gas chromatography coupled with mass spectrometry

BHT:

Butylated hydroxytoluene

PCA:

Principal component analysis

PLS-DA:

Projections to latent structure discriminant analysis

OPLS-DA:

Orthogonal projections to latent structure discriminant analysis

PC:

Phosphatidylcholine

Lyso-PC:

Lysophosphatidylcholine

PE:

Phosphatidylethanolamine

PI:

Phosphatidylinositol

DAG:

Diacylglycerol

TAG:

Triacylglycerol

Cer:

Ceramide

GP:

Glycerophospholipid

SM:

Sphingomyelin

TFAs:

Total fatty acids

FFAs:

Free fatty acids

PUFAs:

Polyunsaturated fatty acids

SFAs:

Saturated fatty acids

MUFAs:

Monounsaturated fatty acids

TC:

Total cholesterol

HDL-C:

High-density lipoprotein cholesterol

GLU:

Glucose

LPL:

Lipoprotein lipase

MAGs:

Monoacylglycerols

FBW:

Final body weight

WGR:

Weight gain rate

SGR:

Specific growth rate

FCR:

Feed conversion ratio

PER:

Protein efficiency rate

SD:

Standard deviation

QC:

Quality control

SF:

Sargassum fucoidan

LCB:

Long-chain base

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Acknowledgements

This work was financially supported by the Natural Science Foundation of Zhejiang Province of China (LY17D060005), Science Technology Department of Zhejiang Province (grant number 2016C37090), Natural Science Foundation of Ningbo of China (2015A 610266), China Spark Program (2015GA701027 and 2015GA701054), and K.C. Wong Magna Fund in Ningbo University. We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

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Authors and Affiliations

  1. School of Marine Sciences, Ningbo University, Room 315, Building Caoguangbiao Technology, #818 Fenghua Road, Ningbo, 315211, China

    Panpan Xu, Juanjuan Chen & Rui Yang

  2. Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Room 314, Building 12, #818 Fenghua Road, Ningbo, 315211, China

    Yajun Wang & Qicun Zhou

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  1. Panpan Xu
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  2. Yajun Wang
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  3. Juanjuan Chen
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  4. Rui Yang
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Correspondence to Yajun Wang or Juanjuan Chen.

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Xu, P., Wang, Y., Chen, J. et al. Lipidomic profiling of juvenile yellow head catfish (Pelteobagrus fulvidraco) in response to Fucoidan diet. Aquacult Int 25, 1123–1143 (2017). https://doi.org/10.1007/s10499-016-0102-1

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