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Effect of galangal (Alpinia galanga Linn.) extract on the expression of immune-related genes and Vibrio harveyi resistance in Pacific white shrimp (Litopenaeus vannamei)

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Abstract

Some plant and herb extracts reportedly possess antimicrobial activities and also have the ability to enhance the nonspecific immune system of shrimp, thereby promoting disease resistance. In this study, a natural herb, galangal (Alpinia galanga Linn.), was used to prevent infectious disease in cultured shrimp, and the effects of galangal–ethanol extract on the expression of the immune-related genes in the shrimp were analyzed via a reverse-transcription polymerase chain reaction of mRNA in circulating hemocytes. Following the intramuscular injection of either galangal extract or trans-p-coumaryl diacetate that had been isolated from galangal rhizome, Pacific white shrimp (Litopenaeus vannamei) showed significant increases in the relative expression ratio of the six immune-related genes compared with a control group. Furthermore, following the oral administration of galangal extract, similar inducible effects of the expression of immune-related genes in the Pacific white shrimp were obtained, which led to an enhanced survival rate from Vibrio harveyi infection. Thus, this study revealed that both galangal extract and trans-p-coumaryl diacetate stimulated the immune system response, thereby promoting resistance to V. harveyi infection in Pacific white shrimp.

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References

  • Bendjeddou D, Lalaoui K, Satta D (2003) Immunostimulating activity of the hot water-soluble polysaccharide extracts of Anacyclus pyrethrum, Alpinia galanga and Citrullus colocynthis. J Ethnopharmacol 88:155–160

    Article  CAS  PubMed  Google Scholar 

  • Burge EJ, Madigan DJ, Burnett LE, Burnett KG (2007) Lysozyme gene expression by hemocytes of Pacific white shrimp Litopenaeus vannamei, after injection with Vibrio. Fish Shellfish Immunol 22:327–339

    Article  CAS  PubMed  Google Scholar 

  • Chang YP, Liu CH, Wu CC, Chiang CM, Lian JL, Hsieh SL (2012) Dietary administration of zingerone to enhance growth, non-specific immune response, and resistance to Vibrio alginolyticus in Pacific white shrimp (Litopenaeus vannamei) juveniles. Fish Shellfish Immunol 32:284–290

    Article  CAS  PubMed  Google Scholar 

  • Cheng W, Chieu HT, Tsai CH, Chen JH (2005) Effects of dopamine on the immunity of white shrimp Litopenaeus vannamei. Fish Shellfish Immunol 19:375–385

    Article  CAS  PubMed  Google Scholar 

  • Chudiwal AK, Jain DP, Somani RS (2010) Alpinia galangal wild—an overview on phyto-pharmacological properties. Indian J Nat Prod Res 1:143–149

    CAS  Google Scholar 

  • Citarasu T, Sivaram V, Immanuel G, Rout N, Murugan V (2006) Influence of selected Indian immunostimulant herbs against white spot syndrome virus (WSSV) infection in black tiger shrimp, Penaeus monodon with reference to haematogical, biochemical and immunological changes. Fish Shellfish Immunol 21:372–384

    Article  PubMed  Google Scholar 

  • Colwell RR (1984) Vibrios in the environment. Wiley, New York

  • Cowley JA, Cadogan LC, Wongteerasupaya C, Hodgson RAJ, Boonsaeng V, Walker PJ (2004) Multiplex RT-nested PCR differentiation of gill-associated virus (Australia) from yellow head virus (Thailand) of Penaeus monodon. J Virol Methods 117:49–59

  • de Lorgeril J, Saulnier D, Janech MG, Gueguen Y, Bachère E (2005) Identification of genes that are differentially expressed in hemocytes of the Pacific blue shrimp (Litopenaeus stylirostris) surviving an infection with Vibrio penaeicida. Physiol Genomics 21:174–183

    Article  PubMed  Google Scholar 

  • de Pooter HL, Omar MN, Coolaset BA, Schamp NM (1985) The essential oil of greater galanga (Alpinia galanga) from Malaysia. Phytochemistry 24:93–96

    Article  Google Scholar 

  • de-la-RE Vega E, García-Galaz A, Díaz-Cinco ME, Sotelo-Mundo RR (2006) White shrimp (Litopenaeus vannamei) recombinant lysozyme has antibacterial activity against Gram negative bacteria: Vibrio alginolyticus, Vibrio parahemolyticus and Vibrio cholera. Fish Shellfish Immunol 20:405–408

  • Destoumieux D, Munoz M, Cosseau C, Rodriguez J, Bulet P, Comps M, Bachere E (2000) Penaeidins, antimicrobial peptides with chitin-binding activity, are produced and stored in shrimp granulocytes and released after microbial challenge. J Cell Sci 113:461–469

    CAS  PubMed  Google Scholar 

  • Fagutao FF, Maningas MBB, Kondo H, Aoki T, Hirono I (2012) Transglutaminase regulates immune-related genes in shrimp. Fish Shellfish Immunol 32:711–715

    Article  CAS  PubMed  Google Scholar 

  • Harikrishnan R, Balasundaram C, Heo MS (2011a) Review, impact of plant products on innate and adaptive immune system of cultured finfish and shellfish. Aquaculture 317:1–15

    Article  CAS  Google Scholar 

  • Harikrishnan R, Balasundaram C, Jawahar S, Heo MS (2011b) Solanum nigrum enhancement of the immune response and disease resistance of tiger shrimp, Penaeus monodon against Vibrio harveyi. Aquaculture 318:67–73

    Article  Google Scholar 

  • Hikima S, Ji Hikima, Rojtinnakorn J, Hirono I, Aoki T (2003) Characterization and function of kuruma shrimp lysozyme possessing lytic activity against Vibrio species. Gene 316:187–195

    Article  CAS  PubMed  Google Scholar 

  • Holmblad T, Söderhäll K (1999) Celladhesion molecules and antioxidative enzyme in a crustacean, possible role in immunology. Aquaculture 172:111–123

    Article  CAS  Google Scholar 

  • Hsieh TY, Wang JC, Hu CY, Li CT, Kuo CM, Hsieh SL (2008) Effects of Rutin from Toona sinensis on the immune and physiological responses of white shrimp (Litopenaeus vannamei) under Vibrio alginolyticus challenge. Fish Shellfish Immunol 25:581–588

    Article  CAS  PubMed  Google Scholar 

  • Jiravanichpaisal P, Lee SY, Kim YA, Andrén T, Söderhäll I (2007) Antibacterial peptides in hemocytes and hematopoietic tissue from freshwater crayfish Pacifastacus leniusculus: characterization and expression pattern. Dev Comp Immunol 31:441–455

    Article  CAS  PubMed  Google Scholar 

  • Karunasagar ID, Karunasagar IN, Umesha RK (2004) Microbial diseases in shrimp aquaculture. In: Ramaiah N (ed) Marine microbiology. Facets and opportunities. National Institute of Oceanography, Goa, India, pp 121–134

  • Lee SW, Najiah M, Wendy W, Nadirah M (2009) Comparative study on antibiogram of Vibrio spp. isolated from diseased postlarval and marketable-sized white leg shrimp (Litopenaeus vannamei). Front Agric China 3:446–451

    Article  CAS  Google Scholar 

  • Lightner DV (1993) Diseases of culture Penaeid shrimp. In: McVey JP (ed) Handbook of mariculture: crustacean aquaculture. CRC Press, BocaRaton, pp 455–474

  • Liu CH, Yeh SP, Kuo CM, Cheng W, Chou CH (2006) The effect of sodium alginate on the immune response of tiger shrimp via dietary administration: activity and gene transcription. Fish Shelllfish Immunol 21:442–452

    Article  CAS  Google Scholar 

  • Liu H, Jiravanichpaisal P, Cerenius L, Lee BL, Soderhall I, Soderhall K (2007) Phenoloxidase is an important component of the defense against Aeromonas hydrophila infection in a crustacean, Pacifastacus leniusculus. J Biol Chem 282:33593–33598

    Article  CAS  PubMed  Google Scholar 

  • Lo C-F, Ho C-H, Chen C-H, Liu K-F, Chiu Y-L, Yeh P-Y, Peng S-E, Hsu H-C, Liu H-C, Chang C-F, Su M-S, Wang C-H, Koul G-H (1997) Detection and tissue tropism of white spot syndrome baculovirus (WSBV) in captured brooders of Penaeus monodon with a special emphasis on reproductive organs. Dis Aquat Org 30:53–72

    Article  Google Scholar 

  • Maia WJ, Wang WN (2010) Protection of blue shrimp (Litopenaeus stylirostris) against the White Spot Syndrome Virus (WSSV) when injected with shrimp lysozyme. Fish Shellfish Immunol 28:727–733

    Article  Google Scholar 

  • Maningas MB, Kondo H, Hirono I, Saito-Taki T, Aoki T (2008) Essential function of transglutaminase and clotting protein in shrimp immunity. Mol Immunol 45:1269–1275

    Article  CAS  PubMed  Google Scholar 

  • Mayachiew P, Devahastin S (2008) Antimicrobial and antioxidant activities of Indian gooseberry and galangal extracts. Food Sci Technol 41:1153–1159

    CAS  Google Scholar 

  • Min HJ, Nam JW, Yu ES, Hong JH, Seo EK, Hwang ES (2009) Effect of naturally occurring hydroxychavicol acetate on the cytokine production in T helper cells. Int Immunopharmacol 9:448–454

    Article  CAS  PubMed  Google Scholar 

  • Montero AB, Austin B (1999) Characterization of extracellular products from an isolate of Vibrio harveyi recovered from diseased post-larval Penaeus vannamei (Bonne). J Fish Dis 22:377–386

    Article  CAS  Google Scholar 

  • Navarro SA, Tang KFJ, Lightner DV (2009) An improved Taura syndrome virus (TSV) RT-PCR using newly designed primers. Aquaculture 293:290–292

    Article  CAS  Google Scholar 

  • Neves CA, Santos EA, Bainy ACD (2000) Reduce superoxide dismutase activity in Palaemonetes argentinus (Decapoda, Palemonidae) infected by Probopyrus ringueleti (Isopoda, Bopyridae). Dis Aquat Org 39:155–158

    Article  CAS  PubMed  Google Scholar 

  • Nunan LM, Lightner DV (2011) Optimized PCR assay for detection of white spot syndrome virus (WSSV). J Virol Methods 171:318–321

    Article  CAS  PubMed  Google Scholar 

  • Oonmetta-aree J, Suzuki T, Gasaluck P, Eumkeb G (2006) Antimicrobial and action of galangal (Alpinia galanga Linn.) on Staphylococus aureus. Food Sci Technol 39:959–965

    Google Scholar 

  • Ponprateep S, Somboonwiwat K, Tassanakajon A (2009) Recombinant anti-lipopolysaccharide factor isoform 3 and prevention of vibriosis in the black tiger shrimp, Penaeus monodon. Aquaculture 289:219–224

    Article  CAS  Google Scholar 

  • Rao K, Ch B, Narasu LM, Giri A (2010) Antibacterial Activity of Alpinia galangal (L) Wild Crude Extracts. Appl Biochem Biotechnol 62:871–884

    Article  Google Scholar 

  • Ruangpan L (1998) Luminous bacteria associated with shrimp mortality. In: Flegel TW (ed) Advances in shrimp biotechnology. National Center for Genetic Engineering and Biotechnology, Bangkok, pp 205–211

    Google Scholar 

  • Ruangpan L, Kitao T (1991) Vibrio bacteria isolated from black tiger shrimp Penaeus monodon Fabricius. J Fish Dis 14:383–388

    Article  Google Scholar 

  • Shockey JE, Leary NA, de la Vega E, Browdy CL, Baatz JE, Gross PS (2009) The role of crustins in Litopenaeus vannamei in response to infection with shrimp pathogens: an in vivo approach. Dev Comp Immunol 33:668–673

    Article  CAS  PubMed  Google Scholar 

  • Söderhäll K, Hall L (1984) Lipopolysaccharide-induced activation of prophenoloxidase activating system in crayfish haemocyte lysate. Biochem Biophys Acta 797:99–104

    Article  Google Scholar 

  • Somboonwiwat K, Supungul P, Rimphanitchayakit V, Aoki T, Hirono I, Tassanakajon A (2006) Differentially expressed genes in haemocytes of Vibrio harveyi-challenged Shrimp Penaeus monodon. Biochem Mol Biol 39:26–36

    Article  CAS  Google Scholar 

  • Sung HH, Hsu SF, Chen CK, Ting YY, Chao WL (2001) Relationships between disease outbreaks in cultured tiger shrimp (Penaeus monodon) and composition of Vibrio communities in pond water and shrimp hepatopancreas during cultivation. Aquaculture 192:101–110

    Article  Google Scholar 

  • Supungul P, Klinbunga S, Pichyangkura R, Hirono I, Aoki T, Tassanakajon A (2004) Antimicrobial peptides discovered in the Black Tiger Shrimp Penaeus monodon using the EST approach. Dis Aquat Org 61:123–135

    Article  CAS  PubMed  Google Scholar 

  • Tang KFJ, Navarro SA, Lightner DV (2007) A PCR assay for discriminating between infectious hypodermal and hematopoietic necrosis virus (IHHNV) and virus-related sequences in the genome of Penaeus monodon. Dis Aquat Org 74:165–170

    Article  CAS  PubMed  Google Scholar 

  • Turker A, Usta C (2002) Biological activity of some medicinal plants sold in Turkish Health—Food stores. Biodivers Ecosyst 34:105–113

    Google Scholar 

  • Vandenberghe J, Verdonck L, Robles-Arozarena R, Rivera G, Blland A, Balladares M, Gomez-Gil B, Calderon J, Sorgeloos P, Swings J (1999) Vibrios Associated with Litopenaeus vannamei Larvae, Postlarvae, Broodstock, and Hatchery Probionts. Appl Environ Microbiol 65:2592–2597

    CAS  PubMed Central  PubMed  Google Scholar 

  • Vargas-Albores F, Yepiz-Plascencia G, Jiménez-Vega F, Ávila-Villa A (2004) Structural and functional differences of Litopenaeus vannamei crustins. Comp Biochem Physiol B Biochem Mol Biol 138:415–422

    Article  PubMed  Google Scholar 

  • Wang YC, Chang PS, Chen HY (2007) Tissue expressions of nine genes important to immune defence of the Pacific white shrimp Litopenaeus vannamei. Fish Shellfish Immunol 23:1161–1177

    Article  PubMed  Google Scholar 

  • Yeh RY, Shiu YL, Shei SC, Cheng SC, Huang SY, Lin JC, Liu CH (2009) Evaluation of the antibacterial activity of leaf and twig extracts of stout camphor tree, Cinnamomum kanehirae, and the effects on immunity and disease resistance of white shrimp, Litopenaeus vannamei. Fish Shellfish Immunol 27:26–32

    Article  PubMed  Google Scholar 

  • Yu ES, Min HJ, Lee K, Lee MS, Nam JW, Seo EK, Hong JH, Hwang ES (2009) Anti-inflammatory activity of p-coumaryl alcohol-y-O-methyl ether is mediated through modulation of interferon-y production in Th cells. Br J Pharmacol 156:1107–1114

    Article  CAS  PubMed Central  PubMed  Google Scholar 

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Acknowledgments

This research was supported by Department of Fisheries, Agriculture Research Development Agency (ARDA), Ministry of Agriculture and Cooperatives, Thailand, and Kyoto Institute of Technology (KIT), Japan.

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Authors and Affiliations

  1. Chanthaburi Coastal Fisheries Research and Development Center, Mu 9, Bangkaja, Muang, Chanthaburi, 22000, Thailand

    Tidaporn Chaweepack, Surachart Chaweepack, Boonyee Muenthaisong & Lila Ruangpan

  2. Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan

    Kei Nagata & Kaeko Kamei

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  1. Tidaporn Chaweepack
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  2. Surachart Chaweepack
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  3. Boonyee Muenthaisong
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Correspondence to Tidaporn Chaweepack.

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Chaweepack, T., Chaweepack, S., Muenthaisong, B. et al. Effect of galangal (Alpinia galanga Linn.) extract on the expression of immune-related genes and Vibrio harveyi resistance in Pacific white shrimp (Litopenaeus vannamei). Aquacult Int 23, 385–399 (2015). https://doi.org/10.1007/s10499-014-9822-2

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