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Comparative growth of Pacific oyster (Crassostrea gigas) postlarvae with microfeed and microalgal diets

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Abstract

Two trials were carried out in the laboratory in order to assess the effect of microparticulated feed (F) and live (Thalassiosira pseudonana, M) diets on the growth of recently set (396 ± 13 μm shell height) and 2 mm Crassostrea gigas postlarvae. Different proportions of M and F (100:0, 75:25, 50:50; 25:75, 0:100) were delivered in a single dose of 3 h d−1 in trial 1. Dietary M:F proportions of 100:0, 50:50, and 0:100 were delivered as a single pulse of 8 h d−1 (P1) or two pulses of 4 h−1 (P2) in trial 2. Maximal daily M ration was 296 cells μl−1 d−1 (trial 1), 150 M cells μl−1 d−1 (trial 2), or their equivalent F dry weight. Shell height (SH), dry (DW), and organic weight (AFDW) were evaluated weekly. Oysters from trial 1 significantly increased their size after 28 days, and exhibited no significant dietary differences in terms of DW (1.21 ± 0.15 to 2.01 ± 0.28 mg) or AFDW (0.091 ± 0.022 to 0.166 ± 0.029 mg). Newly set postlarvae (trial 2) also exhibited significant growth after 25 days. No dietary differences were observed in trial 2, yet P2 oysters attained significantly higher shell heights (825–912 μm) than P1 oysters (730–766 μm) after 25 d. Pulse effects were marginally not significant in terms of AFDW and growth rate. Together, these findings showed that balanced microfeeds have a practical potential for the culture of early C. gigas postlarvae, when they are delivered in pulse-feeding schemes

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Acknowledgments

This study was carried out with funding from the 10a. Convocatoria Interna de Apoyo a proyectos de Investigación UABC under grant no. 0574. The manuscript was greatly improved by suggestions made by two anonymous reviewers.

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Authors and Affiliations

  1. Instituto de Investigaciones Oceanologicas, Universidad Autonoma de Baja California, Km 103 carretera Tijuana-Ensenada, Ensenada, BC, 22860, Mexico

    Circe E. Badillo-Salas, Enrique Valenzuela-Espinoza, Marco A. González-Gómez, Griselda Pares-Sierra, Francisco Ley-Lou & Zaul Garcia-Esquivel

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  1. Circe E. Badillo-Salas
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  2. Enrique Valenzuela-Espinoza
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  3. Marco A. González-Gómez
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  4. Griselda Pares-Sierra
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  5. Francisco Ley-Lou
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  6. Zaul Garcia-Esquivel
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Correspondence to Zaul Garcia-Esquivel.

Appendices

Appendices

Appendix 1a One-way ANOVA on arcsin(sqrt)-transformed survival data from trial 1
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Appendix 1b Two-way ANOVA on arcsin(sqrt)-transformed survival data from trial 2
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Badillo-Salas, C.E., Valenzuela-Espinoza, E., González-Gómez, M.A. et al. Comparative growth of Pacific oyster (Crassostrea gigas) postlarvae with microfeed and microalgal diets. Aquacult Int 17, 173–186 (2009). https://doi.org/10.1007/s10499-008-9189-3

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