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Detection and source identification of airborne extended-spectrum beta-lactamase-producing Escherichia coli isolates in a chicken house

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Abstract

To identify airborne dissemination of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (E. coli) in a chicken house, airborne E. coli was collected from indoor air of a chicken house using six-stage Anderson sampler, and E. coli from chicken fecal samples were also isolated simultaneously. ESBL-producing E. coli isolates from indoor air and fecal samples were screened by a phenotypic confirmatory test according to CLSI recommendations. And then, the enterobacterial repetitive intergenic consensus polymerase chain reaction was performed to analyze the source of airborne ESBL-producing E. coli. The results showed that the ESBL-positive rates of E. coli isolates from feces and the indoor air were 56 % (18/32) and 40 % (6/15), respectively. Furthermore, airborne ESBL-producing E. coli isolates in the chicken house had 100 % genetic similarities with the strains from chicken feces, indicating that ESBL-producing E. coli from chicken feces could be aerosolized and spread to the air.

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Authors and Affiliations

  1. College of Basic Medicine, Taishan Medical University, Tai’an, 271018, Shandong Province, China

    Song Li

  2. Pathology Department, Shandong Provincial Hospital, Jinan, 265200, Shandong Province, China

    Miaoqing Zhao

  3. College of Life Sciences, Taishan Medical University, Tai’an, 271018, Shandong Province, China

    Yanling Li, Ling Zhang, Xianzhong Zhang & Zengmin Miao

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  1. Song Li
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  2. Miaoqing Zhao
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  3. Yanling Li
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  4. Ling Zhang
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  5. Xianzhong Zhang
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  6. Zengmin Miao
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Correspondence to Zengmin Miao.

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Li, S., Zhao, M., Li, Y. et al. Detection and source identification of airborne extended-spectrum beta-lactamase-producing Escherichia coli isolates in a chicken house. Aerobiologia 29, 315–319 (2013). https://doi.org/10.1007/s10453-012-9273-z

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  • DOI: https://doi.org/10.1007/s10453-012-9273-z

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