Annals of Biomedical Engineering

, Volume 44, Issue 6, pp 1863–1880

Chondroinductive Hydrogel Pastes Composed of Naturally Derived Devitalized Cartilage

  • Emily C. Beck
  • Marilyn Barragan
  • Madeleine H. Tadros
  • Emi A. Kiyotake
  • Francisca M. Acosta
  • Sarah L. Kieweg
  • Michael S. Detamore
Emerging Trends in Biomaterials Research

DOI: 10.1007/s10439-015-1547-5

Cite this article as:
Beck, E.C., Barragan, M., Tadros, M.H. et al. Ann Biomed Eng (2016) 44: 1863. doi:10.1007/s10439-015-1547-5

Abstract

Hydrogel precursors are liquid solutions that are prone to leaking from the defect site once implanted in vivo. Therefore, the objective of the current study was to create a hydrogel precursor that exhibited a yield stress. Additionally, devitalized cartilage extracellular matrix (DVC) was mixed with DVC that had been solubilized and methacrylated (MeSDVC) to create hydrogels that were chondroinductive. Precursors composed of 10% MeSDVC or 10% MeSDVC with 10% DVC were first evaluated rheologically, where non-Newtonian behavior was observed in all hydrogel precursors. Rat bone marrow stem cells (rBMSCs) were mixed in the precursor solutions, and the solutions were then crosslinked and cultured in vitro for 6 weeks with and without exposure to human transforming growth factor β3 (TGF-β3). The compressive modulus, gene expression, biochemical content, swelling, and histology of the gels were analyzed. The DVC-containing gels consistently outperformed the MeSDVC-only group in chondrogenic gene expression, especially at 6 weeks, where the relative collagen II expression of the DVC-containing groups with and without TGF-β3 exposure was 40- and 78-fold higher, respectively, than that of MeSDVC alone. Future work will test for chondrogenesis in vivo and overall, these two cartilage-derived components are promising materials for cartilage tissue engineering applications.

Keywords

Devitalized cartilageHydrogelYield stressChondroinduction

Supplementary material

10439_2015_1547_MOESM1_ESM.tif (604 kb)
Supplementary material 1 (TIFF 603 kb)

Copyright information

© Biomedical Engineering Society 2016

Authors and Affiliations

  • Emily C. Beck
    • 1
  • Marilyn Barragan
    • 2
  • Madeleine H. Tadros
    • 3
  • Emi A. Kiyotake
    • 4
  • Francisca M. Acosta
    • 5
  • Sarah L. Kieweg
    • 4
    • 6
  • Michael S. Detamore
    • 4
    • 5
  1. 1.Department of SurgeryUniversity of Kansas Medical CenterKansas CityUSA
  2. 2.Department of Molecular BiosciencesUniversity of KansasLawrenceUSA
  3. 3.Department of Chemical and Biomolecular EngineeringRice UniversityHoustonUSA
  4. 4.Bioengineering ProgramUniversity of KansasLawrenceUSA
  5. 5.Department of Chemical and Petroleum EngineeringUniversity of KansasLawrenceUSA
  6. 6.Department of Mechanical EngineeringUniversity of KansasLawrenceUSA