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Methodology and Evaluation of Intracranial Pressure Response in Rats Exposed to Complex Shock Waves

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Abstract

Studies on blast neurotrauma have focused on investigating the effects of exposure to free-field blast representing the simplest form of blast threat scenario without considering any reflecting surfaces. However, in reality personnel are often located within enclosures or nearby reflecting walls causing a complex blast environment, that is, involving shock reflections and/or compound waves from different directions. The purpose of this study was to design a complex wave testing system and perform a preliminary investigation of the intracranial pressure (ICP) response of rats exposed to a complex blast wave environment (CBWE). The effects of head orientation in the same environment were also explored. Furthermore, since it is hypothesized that exposure to a CBWE would be more injurious as compared to a free-field blast wave environment (FFBWE), a histological comparison of hippocampal injury (cleaved caspase-3 and glial fibrillary acidic protein (GFAP)) was conducted in both environments. Results demonstrated that, regardless of orientation, peak ICP values were significantly elevated over the peak static air overpressure. Qualitative differences could be noticed compared to the ICP response in rats exposed to simulated FFBWE. In the CBWE scenario, after the initial loading the skull/brain system was not allowed to return to rest and was loaded again reaching high ICP values. Furthermore, results indicated consistent and distinct ICP-time profiles according to orientation, as well as distinctive values of impulse associated with each orientation. Histologically, cleaved caspase-3 positive cells were significantly increased in the CBWE as compared to the FFBWE. Overall, these findings suggest that the geometry of the skull and the way sutures are distributed in the rats are responsible for the difference in the stresses observed. Moreover, this increase stress contributes to correlation of increased injury in the CBWE.

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References

  1. Abdul-Muneeret, P. M., H. Schuetz, F. Wang, M. Skotak, J. Jones, S. Gotantla, M. C. Zimmerman, N. Chandra, and J. Haorah. Induction of oxidative and nitrosative damage leads to cerebrovascular inflammation in animal model of mild traumatic brain injury induced by primary blast. Free Radic. Biol. Med. 2013. doi:10.1016/j.freeradbiomed.2013.02.029.

    Google Scholar 

  2. Bauman, R. A., G. Ling, L. Tong, A. Januszkiewicz, D. Agoston, N. Delanerolle, Y. Kim, D. Ritzel, R. Bell, J. Ecklund, R. Armonda, F. Bandak, and S. Parks. An introductory characterization of a combat-casualty-care relevant swine model of closed head injury resulting from exposure to explosive blast. J. Neurotrauma 26:841–860, 2009.

    Article  PubMed  Google Scholar 

  3. Bolander, R., B. Mathie, C. Bir, D. Ritzel, and P. VandeVord. Skull flexure as a contributing factor in the mechanism of injury in the rat when exposed to a shock wave. Ann. Biomed. Eng. 39(10):2550–2559, 2011.

    Article  PubMed  Google Scholar 

  4. Bolander, R. A Multi-Species Analysis of Biomechanical Responses of the Head to a Shock Wave, Detroit, Wayne State University Dissertations, 2012.

  5. Bowen, I. G., E. R. Fletcher, and D. R. Richmond. Estimate of man’s tolerance to the direct effects of air blast. Tech. Report DASA 2113. Washington, DC: Defense Atomic Support Agency, pp. 1–44, 1968.

  6. Cernak, I., A. Merkle, V. Koliatsos, J. Bilik, Q. Luong, T. Mahota, L. Xu, N. Slack, D. Windle, and F. Ahmed. The pathobiology of blast injuries and blast-induced neurotrauma as identified using a new experimental model of injury in mice. Neurobiol. Dis. 41:538–551, 2011.

    Article  PubMed  Google Scholar 

  7. Cernak, I., and L. Noble-Haeusslein. Traumatic brain injury: an overview of pathobiology with emphasis on military populations. J. Cereb. Blood Flow Metab. 30:255–266, 2010.

    Article  PubMed  Google Scholar 

  8. Chavko, M., W. A. Koller, W. K. Prusaczyk, and R. M. McCarron. Measurements of blast wave by miniature fiber optic pressure transducer in the rat brain. J. Neurosci. Methods 159:277–281, 2007.

    Article  PubMed  Google Scholar 

  9. Chavko, M., T. Watanabe, S. Adeeb, J. Lankasky, S. T. Ahlers, and R. M. McCarron. Relationship between orientation to a blast and pressure wave propagation inside the rat brain. J. Neurosci. Methods 195:61–66, 2011.

    Article  PubMed  Google Scholar 

  10. Dal Cengio Leonardi, A. An Investigation of the Biomechanical Response from Shock Wave Loading to the Head, Detroit. Wayne State University Dissertations, 2011.

  11. Dal Cengio Leonardi, A., C. A. Bir, D. V. Ritzel, and P. J. VandeVord. Intracranial pressure increases during exposure to a shock wave. J. Neurotrauma 28:85–94, 2011.

    Article  Google Scholar 

  12. Dal Cengio Leonardi, A., N. J. Keane, C. A. Bir, A. G. Ryan, L. Xu, and P. J. VandeVord. Head orientation affects the intracranial pressure response resulting from shock wave loading in the rat. J. Biomechanics 45(15):2595–2602, 2012.

    Article  Google Scholar 

  13. Ganpule, S., A. Alai, E. Plougonven, and N. Chandra. Mechanics of blast loading on the head models in the study of traumatic brain injury using experimental and computational approaches. Biomech. Model. Mechanobiol. 1–21, 2012. doi:1007/s10237-012-0421-8

  14. Leibovici, D., O. N. Gofrit, M. Stein, S. C. Shapira, Y. Noga, R. J. Heruti, and J. Shemer. Blast injuries: bus versus open-air bombings—a comparative study of injuries in survivors of open-air versus confined-space explosions. J. Trauma Injury Infect. Crit. Care 41(6):1030–1035, 1996.

    Article  CAS  Google Scholar 

  15. Mackenzie, I. M., and B. Tunnicliffe. Blast injuries to the lung: epidemiology and management. Philos. Trans. R. Soc. Lond. B Biol. Sci. 366(1562):295–299, 2011.

    Article  PubMed  Google Scholar 

  16. Mayorga, M. A. The pathology of primary blast overpressure injury. Toxicology 121:17–28, 1997.

    Article  PubMed  CAS  Google Scholar 

  17. Payette, D. J., J. Xie, N. Shirwany, and Q. Guo. Exacerbation of apoptosis of cortical neurons following traumatic brain injury in par-4 transgenic mice. Int. J. Clin. Exp. Pathol. 1(1):44–56, 2008.

    PubMed  CAS  Google Scholar 

  18. Phillips, Y. Y., and D. R. Richmond. Primary blast injury and basic research: a brief history. In: Conventional Warfare: Ballistic, Blast and Burn Injuries, edited by R. F. Bellamy and R. Zajtchuk. Borden Institute of the Office of the Surgeon General of the United States Department of the Army, Chapter 6, 1991, pp. 221–240.

  19. Richmond, D. R., E. G. Damon, E. R. Fletcher, I. G. Bowen, and, C. S. White. The Relationship Between Selected Blast-Wave Parameters and the Response of Mammals Exposed to Air Blast. Annals New York Academy of Sciences, 1968, pp. 103–121.

  20. Richmond, D. R., J. T. Yelverton, E. R. Fletcher, Y. Y. and Phillips. Biological response to complex blast waves. Ninth International Symposium MABS 9, Oxford, England, September 23–27, 1985.

  21. Sajja, V. S., M. P. Galloway, F. Ghoddoussi, D. Thiruthalinathan, A. Kepsel, K. Hay, C. A. Bir, and P. J. VandeVord. Blast-induced neurotrauma leads to neurochemical changes and neuronal degeneration in the rat hippocampus. NMR Biomed. 25(12):1331–1339, 2012.

    Article  PubMed  CAS  Google Scholar 

  22. Saljo, A., F. Bao, A. Hamberger, K. G. Haglid, and H. A. Hansson. Exposure to short-lasting impulse noise causes microglial and astroglial cell activation in the adult rat brain. Pathophysiology 8:105–111, 2001.

    Article  PubMed  CAS  Google Scholar 

  23. Smith, J. E. The epidemiology of blast lung injury during recent military conflicts: a retrospective database review of cases presenting to deployed military hospitals 2003–2009. Philos. Trans. R. Soc. Lond. B Biol. Sci. 366(1562):291–294, 2011.

    Article  PubMed  CAS  Google Scholar 

  24. Sofroniew, M. V. Reactive astrocytes in neural repair and protection. Neuroscientist 11(5):400–407, 2005.

    Article  PubMed  CAS  Google Scholar 

  25. Stuhmiller, J. H. Biological response to blast overpressure: a summary of modeling. Toxicology 121:91–103, 1997.

    Article  PubMed  CAS  Google Scholar 

  26. Sundaramurthy, A., A. Alai, S. Ganpule, A. Holmberg, E. Plougonven, and N. Chandra. Blast-induced biomechanical loading of the rat: an experimental and anatomically accurate computational blast injury model. J. Neurotrauma 29:2352–2364, 2012.

    Article  PubMed  Google Scholar 

  27. VandeVord, P. J., R. Bolander, V. S. Sajja, K. Hay, and C. A. Bir. Mild neurotrauma indicates a range-specific pressure response to low level shock wave exposure. Ann. Biomed. Eng. 40(1):227–236, 2012.

    Article  PubMed  Google Scholar 

  28. Warren, M. W., S. F. Larner, F. H. Kobeissy, C. A. Brezing, J. A. Jeung, R. L. Hayes, M. S. Gold, and K. K. Wang. Calpain and caspase proteolytic markers co-localize with rat cortical neurons after exposure to methamphetamine and MDMA. Acta Neuropathol. 114(3):277–286, 2007.

    Article  PubMed  CAS  Google Scholar 

  29. Yelverton, J. T., D. L. Johnson, W. Hicks, and R. Doyle. Blast overpressure studies with animals and man: non-auditory damage risk assessment for simulated weapons fired from enclosures. Final Report, Contract DAMD 17-88-C-8141, US Army Medical Research & Material Command, Ft. Detrick, MD, 1993.

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Acknowledgments

We would like to thank the WSU Bioengineering Center Staff for assisting with this project, especially Sujith Sajja, Dr. Bin Wu for her indispensable contribution in surgery and Mr. Dave Ritzel for his blast physics expertise. This project was partially funded by the Department of Defense (Award no. W81XWH-08-2-0207) and the Office of Naval Research (Award Number N000140810585).

Conflicts of Interest

No conflicting financial interest exists.

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Authors and Affiliations

  1. Virginia Tech University, Blacksburg, VA, USA

    Alessandra Dal Cengio Leonardi, Anne G. Ryan & Pamela J. VandeVord

  2. Wayne State University, Detroit, MI, USA

    Nickolas J. Keane, Kathryn Hay & Cynthia A. Bir

Authors
  1. Alessandra Dal Cengio Leonardi
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  2. Nickolas J. Keane
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  3. Kathryn Hay
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  4. Anne G. Ryan
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  5. Cynthia A. Bir
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  6. Pamela J. VandeVord
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Corresponding author

Correspondence to Pamela J. VandeVord.

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Associate Editor Michael R. Torry oversaw the review of this article.

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Dal Cengio Leonardi, A., Keane, N.J., Hay, K. et al. Methodology and Evaluation of Intracranial Pressure Response in Rats Exposed to Complex Shock Waves. Ann Biomed Eng 41, 2488–2500 (2013). https://doi.org/10.1007/s10439-013-0850-2

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  • DOI: https://doi.org/10.1007/s10439-013-0850-2

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