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Photonic lab-on-chip (PhLOC) for enzyme-catalyzed reactions in continuous flow

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Abstract

A photonic lab-on-chip (PhLOC) system consisting on an enzymatically-functionalized continuous microfluidic reactor, incorporating up to four interrogation areas for spectrometric detection is here presented. It is a versatile platform suitable for monitoring of enzymatic catalytic reactions, with potential applications in industrial biocatalysis of products of interest, as well as in continuous sensing. Horseradish peroxidase (HRP) was used as a model enzyme and immobilized in specific regions of the PhLOC to demonstrate its operativity in continuous flow. Reaction kinetics were spectrometrically determined for the HRP-catalyzed reduction of H2O2 mediated by a colored substrate (2,2′azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), ABTS), using a continuous flow experimental set-up. Maximum reaction rate was both theoretically and experimentally determined indicating that the immobilization procedure did not affect HRP catalytic properties. In addition, the limit of detection for the enzymatic reaction of the PhLOC was also obtained and found in accordance with previous reported values for other photonic systems operating in non-continuous mode.

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Acknowledgments

This work has been partly funded by the European Commission (Contract No. 317916) under the LiPhos project. The authors thank Dr. Luis David Patiño López for his valuable help with the interferometric experiment.

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Authors and Affiliations

  1. Institut de Microelectrònica de Barcelona (IMB-CNM, CSIC), Campus UAB, 08193, Bellaterra, Spain

    Isaac Rodríguez-Ruiz, Eduard Masvidal-Codina, Tobias N. Ackermann & Andreu Llobera

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  1. Isaac Rodríguez-Ruiz
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  2. Eduard Masvidal-Codina
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  3. Tobias N. Ackermann
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  4. Andreu Llobera
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Correspondence to Andreu Llobera.

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Rodríguez-Ruiz, I., Masvidal-Codina, E., Ackermann, T.N. et al. Photonic lab-on-chip (PhLOC) for enzyme-catalyzed reactions in continuous flow. Microfluid Nanofluid 18, 1277–1286 (2015). https://doi.org/10.1007/s10404-014-1526-4

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  • DOI: https://doi.org/10.1007/s10404-014-1526-4

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