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Interfacing microfluidics to LDI-MS by automatic robotic spotting

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Abstract

We developed a method of interfacing microfluidics with mass spectrometry (MS) using a robotic spotting system to automate the contact spotting process. We demonstrate that direct and automated spotting of analyte from multichannel microfluidic chips to a custom microstructured MALDI target plate was a simple, robust, and high-throughput method for interfacing parallel microchannels using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Using thermoplastic cyclic olefin copolymer (COC) polymer microfluidic chips containing eight parallel 100 μm × 46 μm microchannels connected to a single input port, spotting volume repeatability and MALDI-MS signal uniformity are evaluated for a panel of sample peptides. The COC microfluidic chips were fabricated by hot embossing and solvent bonding techniques followed by chip dicing to create open ends for MS interfacing. Using the automatic robotic spotting approach, microfluidic chip-based reversed-phase liquid chromatography (RPLC) separations were interfaced with electrochemically etched nanofilament silicon (nSi) target substrate, demonstrating the potential of this approach toward chip-based microfluidic separation coupled with matrix-free laser desorption/ionization mass spectrometry.

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Acknowledgments

This work was supported by NIH/NIGMS grant # R01GM072512 and National Science Council, Taiwan grant # NSC 97-2218-E-008-010-MY2.

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Authors and Affiliations

  1. Department of Mechanical Engineering, National Central University, Jhongli, 32001, Taiwan

    Chia-Wen Tsao

  2. Calibrant Biosystems, Gaithersburg, MD, 20878, USA

    Song Tao

  3. Department of Mechanical Engineering, University of Maryland, College Park, MD, 20742, USA

    Chien-Fu Chen, Jikun Liu & Don L. DeVoe

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  1. Chia-Wen Tsao
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  2. Song Tao
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Correspondence to Chia-Wen Tsao.

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Tsao, CW., Tao, S., Chen, CF. et al. Interfacing microfluidics to LDI-MS by automatic robotic spotting. Microfluid Nanofluid 8, 777–787 (2010). https://doi.org/10.1007/s10404-009-0510-x

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  • DOI: https://doi.org/10.1007/s10404-009-0510-x

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