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Rat chronic glaucoma model induced by intracameral injection of microbeads suspended in sodium sulfate–sodium hyaluronate

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Abstract

Purpose

To establish a rat glaucoma model with chronic intraocular pressure (IOP) elevation induced by microbeads suspended in sodium sulfate–sodium hyaluronate.

Methods

Chronic elevation of IOP was induced unilaterally by injecting polystyrene microbeads, suspended in 4 % sodium sulfate and 3 % sodium hyaluronate, into the anterior chamber. The microbead suspension was injected through either the clear corneal (CC) or sclerocorneal (SC) tunnel. IOP changes were monitored up to 8 weeks after injection. The loss of retinal ganglion cells (RGCs) was assessed using fluorogold retrograde labeling of RGCs. RGC axons were evaluated by immunohistochemistry and immunoblotting.

Results

The resulting IOP elevation was maintained up to 3 weeks after the intracameral injection of microbeads through the CC route and up to 4 weeks after injection through the SC route. The density of RGCs was significantly reduced at 4 weeks after injection, with the SC route leading to more RGC loss than the CC route (p = 0.037). The neurofilament immunoreactivity and protein levels in the optic nerve were also significantly reduced at 4 weeks after injection. Some eyes in the SC route cohort received re-injection of the microbead suspension at 4 weeks after the initial injection, which led to an elevated IOP more than 8 weeks after the initial injection, and eventually a 27.5 % loss of RGC density compared with the control eyes.

Conclusion

The intracameral injection of microbeads suspended in hyaluronate effectively produced chronic IOP elevation and subsequent RGC degeneration in rat eyes. The sclerocorneal tunnel approach yielded a longer period of IOP elevation than the clear corneal approach. Our modified microbead injection offers a reliable high-pressure glaucoma model.

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Acknowledgments

The authors thank Sho Nobuyoshi, Keiko Kojima and Asami Kita for experimental assistance. This work was supported by JSPS KAKENHI grant number 25462715 (A.K.) from the Japanese Government, the Suda Memorial Foundation (A.K.), the Mishima Memorial Foundation (A.K.) and the Santan Pharmaceutical Founder Commemoration Ophthalmic Research Fund (A.K.).

Conflicts of interest

Y. Matsumoto, None; A. Kanamori, None; M. Nakamura, None; A. Negi, None.

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Authors and Affiliations

  1. Division of Ophthalmology, Department of Surgery, Kobe University Graduate School of Medicine, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan

    Yoshiko Matsumoto, Akiyasu Kanamori, Makoto Nakamura & Akira Negi

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  1. Yoshiko Matsumoto
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  2. Akiyasu Kanamori
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  3. Makoto Nakamura
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  4. Akira Negi
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Correspondence to Akiyasu Kanamori.

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Matsumoto, Y., Kanamori, A., Nakamura, M. et al. Rat chronic glaucoma model induced by intracameral injection of microbeads suspended in sodium sulfate–sodium hyaluronate. Jpn J Ophthalmol 58, 290–297 (2014). https://doi.org/10.1007/s10384-014-0311-y

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  • DOI: https://doi.org/10.1007/s10384-014-0311-y

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