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Biodiversity in Indigenous Germplasm of Pyrus from Pakistan Based on Phenotypical and Morphological Traits

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Abstract

A comprehensive analysis of various horticulturally important morphological traits of sixty pear accessions located in five districts of Azad Jammu and Kashmir (Pakistan) was carried out. Several qualitative and quantitative traits were investigated in situ and after the harvesting of leaf and fruit samples. The rich diversity was observed for qualitative traits (i. e. growth habit, blooming time, intensity of flowering, fruit setting, ripening time, productivity, and fruit shape and color) among pear accessions. As for the quantitative traits, significant variation was observed for leaf area (8.96–27.17 cm2), flowering duration (11–24 days), fruit weight (68.1–322.3 g), average fruit length (2.68–9.87 cm) and average fruit width (1.82–8.12 cm) between pear accessions. Based on qualitative and quantitative data, two dendograms were constructed by using cluster analysis which separated pear accessions into three distinct groups. The cluster analysis showed considerable differences between the accessions for qualitative and quantitative traits. Furthermore, a close association between accessions was noted inside the clusters for qualitative and quantitative traits. The results showed rich diversity and similarity between the pear accessions and needs further assessment at molecular level to elucidate their phylogenetic relationship.

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References

  • Agrimonti C, Bianchi R, Bianchi A, Ballero M, Poli F, Marmiroli N (2007) Understanding biological conservation strategies: a molecular-genetic approach to the case of myrtle (Myrtus communis L.) in two Italian regions: Sardinia and Calabria. Conser Gen 8:385–396

    Article  CAS  Google Scholar 

  • Ahmed M, Anjum MA, Mohib R, Naz M, Khan MR, Hussain S (2013) Characterization of indigenous barberry germplasm in Pakistan: variability in morphological characteristics and nutritional composition. Fruits 68:409–422

    Article  CAS  Google Scholar 

  • Ahmed M, Anjum MA, Khaqan K, Hussain S (2014) Biodiversity in morphological and physico-chemical characteristics of wild raspberry (Rubus idaeus L.) germplasm collected from temperate region of Azad Jammu & Kashmir (Pakistan). Acta Sci Pol Hortorum Cultus 13:117–134

    Google Scholar 

  • Armstrong PR, Beudry RM, Brown GK (1993) Non-destructive firmness testing on the tree during maturation. ASABE paper, vol. 93–6540. ASABE, St. Joseph, Michigan

    Google Scholar 

  • Aulakh PS, Dhaliwal GS, Jawanda JS (1981) Investigation on floral biology in pear (Pyrus communis L.): studies on flower bud development, flower anthesis and flowering density in pears. Pun Hort J 21:175–181

    Google Scholar 

  • Behboudian MH, Lawes GS, Griffiths KM (1994) The influence of water deficit on water relations, photosynthesis and fruit growth in Asian pear (Pyrus serotina Rehd.). Sci Hortic 60:89–99

    Article  Google Scholar 

  • Bell RL, Quamme HA, Layne REC, Skirvin RN (1996) Pears. In: Janick J, Moore JN (eds) Tree and tropical fruits. Fruit Breeding, vol 1. John Wiley & Sons, New York, pp 441–514

    Google Scholar 

  • Campbell CS, Evans RC, Morgan DR, Dickinson TA, Arsenault MP (2007) Phylogeny of subtribe Pyrinae (formerly the Maloideae, Rosaceae): limited resolution of a complex evolutionary history. Plant Syst Evol 266:119–145

    Article  CAS  Google Scholar 

  • Caspari HW, Behboudian MH, Chalmers DG (1994) Water use, growth, and fruit yield of ‘Housi’ Asian pears under deficit irrigation. J Am Soc Hortic Sci 119:383–388

    Google Scholar 

  • Cheon KW, Sung HH, Sheob SI, Uk SY, Kyun LD, Jo KS, Youl MJ, Ho KJ (2001) Breeding of a new late-season pear cultivar ‘Mansoo’ with large-sized, high quality and long storability. Kor J Hortic Sci Tech 19:66–70

    Google Scholar 

  • Cowan AK, Cripps RF, Richings EW, Taylor NJ (2001) Fruit size: towards an understanding of the metabolic control of fruit growth using avocado as a model system. Physiol Plant 111:127–136

    Article  CAS  Google Scholar 

  • Dumanğlu H, Tuna-Güneş N, Erdoğan V, Ayğun A, Şan B (2006) Clonal selection of a winter-type European pear cultivar ‘Ankara’ (Pyrus communis L.). Turk J Agri For 30:355–363

    Google Scholar 

  • Erez A, Flore JA (1986) The quantitative effect of solar radiation on “Redhaven” peach fruit skin colour. HortScience 21:1424–1426

    Google Scholar 

  • Garriz PI, Colavita GM, Alvarez HL (1998) Fruit and spur leaf growth and quality as influenced by low irradiance levels in pear. Sci Hortic 77:195–205

    Article  Google Scholar 

  • Gupta MR, Mehrotra NK (1985) Studies on pollination in pear cultivars. J Res Pun Agri Uni 22:463–466

    Google Scholar 

  • Hammer K, Arrowsmith N, Gladis T (2003) Agrobiodiversity with emphasis on plant genetic resources. Naturwissenschaften 90:241–250

    Article  CAS  PubMed  Google Scholar 

  • Katayama H, Uematsu C (2006) Pear (Pyrus species) genetic resources in Iwate. Jpn Gen Resour Crop Ev 53:483–498

    Article  Google Scholar 

  • Kimura T, Shi YZ, Shoda M, Kotobuki K, Matsuda N, Hayashi T, Ban Y, Yamamoto T (2002) Identification of asian pear varieties by SSR analysis. Breed Sci 52:115–121

    Article  CAS  Google Scholar 

  • Koehler-Santos P, Dornelles ALC, De Frietas JB (2003) Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analysis. Pesqui Agropecu Bras 38:797–906

    Article  Google Scholar 

  • Kumar N, Dimri DC, Nigam JK (2004) Studies on flowering, fruit set and growth pattern of some promising peach cultivars grown under humid temperate mid hills of Uttaranchal. Ind J Hort 61:271–272

    Google Scholar 

  • Layne REC, Quamme HA (1975) Pears. In: Janick J, Moore JN (eds) Advances in fruit breeding. Purdue University Press, West Lafayette, pp 38–70

    Google Scholar 

  • Lone AF, Wafal BA (2000) Varietal diversity in the germplasm of cherries under cultivation in Kashmir. In: Khan MA, Farooq S (eds) Environmental biodiversity and conservation. A.P.H. Publishing Corporation, New Delhi, pp 319–340

    Google Scholar 

  • Lotze E, Bergh O (2004) Early prediction of harvest fruit size distribution of an apple and pear cultivar. Sci Hortic 101:281–290

    Article  Google Scholar 

  • Mantel N (1967) The detection of disease clustering and generalized regression approach. Cancer Res 27:209–220

    CAS  PubMed  Google Scholar 

  • Marini RP, Sowers D, Marini MC (1991) Peach fruit quality is affected by shade during final swell of fruit growth. J Am Soc Hortic Sci 116:383–389

    Google Scholar 

  • Martinez L, Cavagnaro P, Masuelli R, Rodriguez J (2003) Evaluation of diversity among Argentine grapevine (Vitis vinifera L.) varieties using morphological data and AFLP markers. Electron J Biotechnol 16:11

    Google Scholar 

  • Milutinovic MD, Djakovic G, Militinivic MM, Miletic R, Zikic M (2005) Variability in the wild pear population in West Serbia. Acta Hortic 671:247–251

    Article  Google Scholar 

  • Mitchell PD, Jerie PH, Chalmers DJ (1984) The effects of regulated water deficits on pear tree growth, flowering, fruit growth and yield. J Am Soc Hortic Sci 109:604–606

    Google Scholar 

  • Mohammadi SA, Prasanna BM (2003) Analysis of genetic diversity in crop plants-salient statistical tools and considerations. Crop Sci 43:1235–1248

    Article  Google Scholar 

  • Monte-Corvo L, Goulão L, Oliveira C (2001) ISSR analysis of cultivars of pear and suitability of molecular markers for clone discrimination. J Am Soc Hortic Sci 126:517–522

    CAS  Google Scholar 

  • Nisar H, Ahmed M, Anjum MA, Hussain S (2015a) Genetic diversity in fruit nutritional composition, anthocyanins, phenolics and antioxidant capacity of plum (Prunus domestica) genotypes. Acta Sci Pol Hortorum Cultus 14:45–61

    Google Scholar 

  • Nisar H, Ahmed M, Anjum MA, Hussain S (2015bv) Biodiversity in morpho-physiological characteristics of indigenous plum germplasm from Azad Jammu and Kashmir, Pakistan. Zemdirbyste Agric 102:423–430

    Article  Google Scholar 

  • Paganova V (2003) Taxonomical reliability of leaf and fruit morphological characteristic of the Pyrus L. taxa in Slovakia. HortScience 3:98–107

    Google Scholar 

  • Palmer JW (1981) Computed effects of spacing on light interception and distribution within hedgerow trees in relation to productivity. Acta Hort 114:80–88

    Article  Google Scholar 

  • RHS (2001) Colour chart. Royal Horticultural Society, London, p 4

    Google Scholar 

  • Robertson KR, Phipps JB, Rohrer JR, Smith PG (1991) A synopsis of genera in Maloideae (Rosaceae). Syst Bot 16:376–394

    Article  Google Scholar 

  • Rohlf FJ (2004) NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System, Version 2.2. Exeter Publishing, Setauket

    Google Scholar 

  • Rotondi A, Magli M, Ricciolini C, Baldoni L (2003) Morphological and molecular analyses for the characterization of a group of Italian olive cultivars. Euphytica 132:129–137

    Article  CAS  Google Scholar 

  • Sharifani M, Hemmati K, Hassani S, Fallahi E (2006) Evaluation of useful botanical traits for iranian Pyrus species. international symposium on Asian plants with unique horticultural potential. Acta Hortic 769:185–188

    Google Scholar 

  • Singh SK, Bist LD, Patel VB (2004) Flowering and floral biology studies in low chill pear cultivars. Ind J Hort 61:94–96

    Google Scholar 

  • Sneath PHA, Sokal RR (1973) Numerical taxonomy: the principles and practice of numerical classification. W. H. Freeman & Co., San Francisco, p 573

    Google Scholar 

  • Stanley CJ, Tustin DS, Lupton GB, McArthney S, Cashmore WM, de Silva HN (2000) Towards understanding the role of temperature in apple fruit growth responses in three geographical regions within New Zealand. J Hortic Sci Bio 5:413–422

    Google Scholar 

  • Thibault B, Watkins R, Smith RA (1983) Descriptor list for pear (Pyrus). International board for plant genetic resources. IBPGR 82(57):6–35

    Google Scholar 

  • Thompson M (1988) Bio-geographical survey and collection of temperate fruit and nut genetic resources in Northern Pakistan. Trip report. International board for plant genetic resources. IBPGR 88(55):105

    Google Scholar 

  • Verissimo V, Gardin JP, Trevisan R, de Silva JB (2002) Morphological and physical parameters of flower buds of the trees of two Japanese pear cultivars grown at three different areas of Southern Brazil and their relationship with flower bud abortion intensity. Acta Hortic 587:381–386

    Article  Google Scholar 

  • Watkins R (1976) Cherry plum peach apricot and almond. In: Simmonds NW (ed) Evolution of crop plants. Longman, London, pp 242–247

    Google Scholar 

  • Weiguo Z, Zhihua Z, Xuexia M, Yong Z, Sibao W, Jianhua H, Hui X, Yile P, Yongping H (2007) A comparison of genetic variation among wild and cultivated Morus species (Moraceae: Morus) as revealed by ISSR and SSR markers. Biodivers Conserv 16:275–290

    Article  Google Scholar 

  • Westwood MN (1978) Temperate zone pomology. Freeman W. H., & Co, San Francisco, p 428

    Google Scholar 

  • Zagaja SW (1977) Fruits of North-East China. Fruit Sci Rep 4:1–8

    Google Scholar 

Download references

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Correspondence to Sajjad Hussain.

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Ahmed, M., Anjum, M.A., Hussain, S. et al. Biodiversity in Indigenous Germplasm of Pyrus from Pakistan Based on Phenotypical and Morphological Traits. Erwerbs-Obstbau 59, 19–27 (2017). https://doi.org/10.1007/s10341-016-0291-9

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  • DOI: https://doi.org/10.1007/s10341-016-0291-9

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