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Taxonomic re-examination of several Japanese Stemphylium strains based on morphological and molecular phylogenetic analyses

  • Fungal Diseases
  • Published:
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Abstract

Most of the many Stemphylium species on record as plant pathogens in Japan have been identified by morphology. Using molecular phylogenetic analysis of four loci (rDNA-ITS, EF-1α, GPD, and vmaA-vpsA) combined, we re-examined the taxonomy of 31 Stemphylium strains that had been identified morphologically before or after their deposit in the NIAS Genebank, Japan. In the present study, these Japanese strains were separated into three groups (Groups A, C, and E). Sixteen Japanese strains formed a new subgroup (designated Subgroup C2) that contained no type or ex-type strains of existing species. Four of the strains had been identified correctly, whereas the other 27 strains appear to have been misidentified based on our phylogenetic analysis. We examined the conidial morphology of four representative misidentified strains selected from each clade. While the morphological characteristics of a strain isolated from Welsh onion resembles those of the S. vesicarium type, this strain belongs to Subgroup C2 rather than Subgroup C1 and, therefore, could not be identified at the species level. Conversely, three pathogenic strains isolated from aster, asparagus, and Chinese chive were re-identified based on our combined morphological and phylogenetic data. We propose the scientific names of the aster leaf spot pathogen as S. lycopersici, and the asparagus Stemphylium leaf spot and Chinese chive brown leaf blight pathogens as S. herbarum.

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Acknowledgments

We express our gratitude to Dr. Harry Evans, CABI Europe-UK, for his help with improving this manuscript.

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Author notes

  1. Daisuke Kurose

    Present address: CABI Europe-UK, Bakeham Lane, Egham, Surrey, TW20 9TY, UK

Authors and Affiliations

  1. Natural Resources Inventory Center, National Institute for Agro-Environmental Sciences, Kannondai, Tsukuba, Ibaraki, 305-8604, Japan

    Daisuke Kurose & Seiya Tsushima

  2. Donan Agricultural Experiment Station, Hokkaido Research Organization, Honcho, Hokuto, Hokkaido, 041-1201, Japan

    Tomoo Misawa

  3. National Institute of Agrobiological Sciences, Kannondai, Tsukuba, Ibaraki, 300-8602, Japan

    Takahito Suzui

  4. Yamanashi Prefectural Agritechnology Center, Shimo-imai, Kai, Yamanashi, 400-0105, Japan

    Kazunori Ichikawa

  5. Kagawa Prefectural Agricultural Experiment Station, Ayagawa-machi-kita, Ayauta-gun, Kagawa, 761-2306, Japan

    Gan Kisaki

  6. Department of Molecular Plant Pathology, Agricultural Genetics Institute, Pham Van Dong, Tu liem, Hanoi, Vietnam

    Long H. Hoang

  7. Faculty of Agriculture, Kyushu University, Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Naruto Furuya & Kenichi Tsuchiya

  8. Genetic Resources Center, National Institute of Agrobiological Sciences, Kannondai, Tsukuba, Ibaraki, 305-8602, Japan

    Toyozo Sato

Authors
  1. Daisuke Kurose
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  2. Tomoo Misawa
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  3. Takahito Suzui
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  4. Kazunori Ichikawa
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  5. Gan Kisaki
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  6. Long H. Hoang
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  8. Kenichi Tsuchiya
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  9. Seiya Tsushima
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  10. Toyozo Sato
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Corresponding author

Correspondence to Toyozo Sato.

Additional information

The nucleotide sequence data reported in this paper are available in the DDBJ/EMBL/GenBank databases as accessions AB704310 and AB979876–AB979994.

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Kurose, D., Misawa, T., Suzui, T. et al. Taxonomic re-examination of several Japanese Stemphylium strains based on morphological and molecular phylogenetic analyses. J Gen Plant Pathol 81, 358–367 (2015). https://doi.org/10.1007/s10327-015-0607-x

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  • DOI: https://doi.org/10.1007/s10327-015-0607-x

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