Abstract
The emergence of Magnaporthe oryzae strains with resistance to quinone outside inhibitor (QoI) fungicides necessitates the development of easy techniques for the rapid, sensitive monitoring of such strains. A QoI-resistant mutant and a wild-type strain showed a single nucleotide polymorphism (SNP). For simple, fast, low-cost, and reliable detection of this SNP, we developed two types of PCR-based DNA markers that enable easy detection of resistant strains by using DNA templates prepared from filter paper permeated with the fungus. The method enabled us to obtain reliable results by using a detection procedure that takes only a few hours without special kits.
References
Araki Y, Sugihara M, Sawada H, Fujimoto H, Masuko M (2005) Monitoring of the sensitivity of Magnaporthe grisea to metominostrobin 2001–2003: no emergence of resistant strains and no mutations at codon 143 or 129 of the cytochrome b gene. J Pestic Sci 30:203–208
Bartlett DW, Clough JM, Godwin JR, Hall AA, Hamer M, Parr-Dobrzanski B (2002) The strobilurin fungicides. Pest Manag Sci 58:649–662
Delgado JA, Lynnes TC, Meinhardt SW, Wise KA, Gudmestad NC, Bradley CA, Markell SG, Goswami RS (2013) Identification of the mutation responsible for resistance to QoI fungicides and its detection in Ascochyta rabiei (teleomorph Didymella rabiei). Plant Pathol 62:688–697
Fontaine S, Remuson F, Fraissinet-Tachet L, Micoud A, Marmeisse R, Melayah D (2009) Monitoring of Venturia inaequalis harbouring the QoI resistance G143A mutation in French orchards as revealed by PCR assays. Pest Manag Sci 65:74–81
Hayano-Saito Y, Hayashi K, Ashizawa T, Suzuki F (2015) Paper-disc method: a simple DNA preparation from the rice blast fungus for PCR (in Japanese with English summary). Jpn J Phytopathol 81 (in press)
Hayashi K, Hashimoto N, Daigen M, Ashikawa I (2004) Development of PCR-based SNP markers for rice blast resistance genes at the Piz locus. Theor Appl Genet 108:1212–1220
Hirooka T, Ishii H (2013) Chemical control of plant diseases. J Gen Plant Pathol 79:390–401
Ishii H (2006) Impact of fungicide resistance in plant pathogens on crop disease control and agricultural environment. Jpn Agric Res Q 40:205–211
Ishii H (2012) Resistance to QoI and SDHI fungicides in Japan. In: Thind TS (ed) Fungicide resistance in crop protection: risk and management. CABI, Wallingford, pp 223–234
Kim Y-S, Dixon EW, Vincelli P, Farman ML (2003) Field resistance to strobilurin (QoI) fungicides in Pyricularia grisea caused by mutations in the mitochondrial cytochrome b gene. Phytopathology 93:891–900
Miyagawa N, Fuji M (2013) Occurrence of QoI-fungicide-resistant strains of Magnaporthe oryzae on rice and fungicidal effective (in Japanese with English summary). In: Abstracts of the 23rd symposium of research committee on fungicide resistance, Gifu, Japan, pp 25–35
Stammler G, Itoh M, Hino I, Watanabe A, Kojima K, Motoyoshi M, Koch A, Haden E (2007) Efficacy of orysastrobin against blast and sheath blight in transplanted rice. J Pestic Sci 32:10–15
Takagaki M, Sugihara M, Kimura N (2004) Mechanism of reduced sensitivity to melanin biosynthesis inhibitors targeting scytalone dehydratase (MBI-D) in Magnaporthe grisea and gene diagnostic method for testing the sensitivity (in Japanese). Plant Protect 58:24–28
Wei C-Z, Katoh H, Nishimura K, Ishii H (2009) Site-directed mutagenesis of the cytochrome b gene and development of diagnostic methods for identifying QoI resistance of rice blast fungus. Pest Manag Sci 65:1344–1351
Acknowledgments
This work was supported by a Grant from the Ministry of Agriculture, Forestry, and Fisheries of Japan (Genomics-based Technology for Agricultural Improvement, Grant No. PRM-5001).
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Hayashi, K., Ashizawa, T., Sasaya, T. et al. Rapid PCR technique to detect QoI-resistant strains of Magnaporthe oryzae . J Gen Plant Pathol 81, 131–135 (2015). https://doi.org/10.1007/s10327-014-0573-8
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DOI: https://doi.org/10.1007/s10327-014-0573-8