Abstract
Sugar beet (Beta vulgaris L. subsp. vulgaris var. altissima) is a biennial, sugar-producing tuber crop grown in different parts of the world. Diseased sugar beets with symptoms of little leaf were observed during 2008 in Tamil Nadu, India. Phytoplasmas were detected in symptomatic leaves of three separate plants using PCR with primer pair fU5/rU3, which amplify an 880-bp fragment of the 16S rRNA region of phytoplasmas. The nucleotide sequence analysis of a 766-bp fragment had 100% identity among the sequence from the three plants (GQ184437) and 96% nucleotide sequence identity with 16S ribosomal RNA gene sequences of eggplant phyllody phytoplasma.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bressan A, Semetey O, Nusillard B, Boudon-Padieu E (2007) The syndrome “Basses Richesses” of sugar beet in France is associated with different pathogen types and insect vectors. B Insectol 60:395–396
Edwin BT, Mohankumar C (2007) Kerala wilt disease phytoplasma: phylogenetic analysis and identification of a vector, Proutista moesta. Physiol Mol Plant Pathol 71:41–47
Gatineau F, Larrue J, Clair D, Lorton F, Richard-Molard M, Boudon-Padieu E (2001) A new natural planthopper vector of stolbur phytoplasma in the genus Pentastiridius (Hemiptera: Cixiidae). Eur J Plant Pathol 107:263–271
Gatineau F, Jacob N, Vautrin S, Larrue J, Lherminier J, Richard-Molard M, Boudon-Padieu E (2002) Association with the syndrome "basses richesses" of sugar beet of a phytoplasma and a bacterium-like organism transmitted by a Pentastiridius sp. Phytopathology 92:384–392
Köchl S, Niederstätter H, Parson W (2005) DNA extraction and quantitation of forensic samples using the phenol–chloroform method and real-time PCR. Methods Mol Biol 297:13–30
Leilah AA, Badawi MA, Said EM, Ghonema MH, Abdou MAE (2005) Effect of planting dates, plant population and nitrogen fertilization on sugarbeet productivity under the newly reclaimed sandy soils in Egypt. Sci J King Faisal Univ, Basic Appl Sci 3:95–110
Lorenz KH, Schneider B, Ahrens U, Seemüller E (1995) Detection of the apple proliferation and pear decline phytoplasmas by PCR amplification of ribosomal and nonribosomal DNA. Phytopathology 85:771–776
Mumford RA, Potyondi L, Harju VA, Henry CM (2000) The identification of a phytoplasma from the Aster Yellows group infecting sugar beet in Hungary. Plant Pathol 49:806
Sharmila LB, Bhasker S, Thelly MT, Edwin BT, Mohankumar C (2004) Cloning and sequencing of phytoplasma ribosomal DNA (rDNA) associated with Kerala wilt disease of coconut palms. J Plant Biochem Biotechnol 13:1–5
Thompson JD, Higgins DG, Gibson TJ (1994) Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
Acknowledgment
The authors thank the M/S Syngenta India limited for the financial support to carry out this study.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Thilagavathi, R., Ramjegathesh, R., Rajendran, L. et al. First report of little leaf disease associated with phytoplasma on sugar beet (Beta vulgaris L. subsp. vulgaris var. altissima Doll) in India. J Gen Plant Pathol 77, 139–141 (2011). https://doi.org/10.1007/s10327-011-0294-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10327-011-0294-1