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Comparison of characterization and microbial communities in rice straw- and wheat straw-based compost for Agaricus bisporus production

  • Environmental Microbiology
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Rice straw (RS) is an important raw material for the preparation of Agaricus bisporus compost in China. In this study, the characterization of composting process from RS and wheat straw (WS) was compared for mushroom production. The results showed that the temperature in RS compost increased rapidly compared with WS compost, and the carbon (C)/nitrogen (N) ratio decreased quickly. The microbial changes during the Phase I and Phase II composting process were monitored using denaturing gradient gel electrophoresis (DGGE) and phospholipid fatty acid (PLFA) analysis. Bacteria were the dominant species during the process of composting and the bacterial community structure dramatically changed during heap composting according to the DGGE results. The bacterial community diversity of RS compost was abundant compared with WS compost at stages 4–5, but no distinct difference was observed after the controlled tunnel Phase II process. The total amount of PLFAs of RS compost, as an indicator of microbial biomass, was higher than that of WS. Clustering by DGGE and principal component analysis of the PLFA compositions revealed that there were differences in both the microbial population and community structure between RS- and WS-based composts. Our data indicated that composting of RS resulted in improved degradation and assimilation of breakdown products by A. bisporus, and suggested that the RS compost was effective for sustaining A. bisporus mushroom growth as well as conventional WS compost.

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References

  1. Bååth E (2003) The use of neutral lipid fatty acids to indicate the physiological conditions of soil fungi. Microb Ecol 45:373–383

    Article  PubMed  Google Scholar 

  2. Bremner JM, Mulvaney C (1982) Nitrogen-total 1. In: Page AL (ed) Methods of soil analysis. Part 2. Chemical and microbiological properties. American Society for Agronomy, Medison, pp 595–624

    Google Scholar 

  3. Córdova-Kreylos AL, Cao Y, Green PG, Hwang H-M, Kuivila KM, LaMontagne MG, Van De Werfhorst LC, Holden PA, Scow KM (2006) Diversity, composition, and geographical distribution of microbial communities in california salt marsh sediments. Appl Environ Microbiol 72:3357–3366

    Article  PubMed  PubMed Central  Google Scholar 

  4. Cahyani VR, Matsuya K, Asakawa S, Kimura M (2003) Succession and phylogenetic composition of bacterial communities responsible for the composting process of rice straw estimated by PCR-DGGE analysis. Soil Sci Plant Nutr 49:619–630

    Article  CAS  Google Scholar 

  5. Carpenter-Boggs L, Kennedy AC, Reganold JP (1998) Use of phospholipid fatty acids and carbon source utilization patterns to track microbial community succession in developing compost. Appl Environ Microbiol 64:4062–4064

    CAS  PubMed  PubMed Central  Google Scholar 

  6. De Groot PWJ, Basten D, Sonnenberg ASM, Van Griensven L, Visser J, Schaap PJ (1998) An endo-1,4-beta-xylanase-encoding gene from Agaricus bisporus is regulated by compost-specific factors. J Mol Biol 277:273–284

    Article  PubMed  Google Scholar 

  7. Dees PM, Ghiorse WC (2001) Microbial diversity in hot synthetic compost as revealed by PCR-amplified rRNA sequences from cultivated isolates and extracted DNA. FEMS Microbiol Ecol 35:207–216

    Article  CAS  PubMed  Google Scholar 

  8. Dungait JAJ, Kemmitt SJ, Michallon L, Guo S, Wen Q, Brookes PC, Evershed RP (2011) Variable responses of the soil microbial biomass to trace concentrations of 13C-labelled glucose, using 13C-PLFA analysis. Eur J Soil Sci 62:117–126

    Article  CAS  Google Scholar 

  9. Eichner CA, Erb RW, Timmis KN, Wagner-Döbler I (1999) Thermal gradient gel electrophoresis analysis of bioprotection from pollutant shocks in the activated sludge microbial community. Appl Environ Microbiol 65:102–109

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Eiland F, Klamer M, Lind AM, Leth M, Baath E (2001) Influence of initial c/n ratio on chemical and microbial composition during long term composting of straw. Microbiol Ecol 41:272–280

    Article  CAS  Google Scholar 

  11. Fan F (2007) Method of detecting water-soluble carbon in organic fertilizer. China Meas Technol 33:63–64 (in Chinese)

    Google Scholar 

  12. Foulongne-Oriol M, Spataro C, Savoie J-M (2009) Novel microsatellite markers suitable for genetic studies in the white button mushroom Agaricus bisporus. Appl Microbiol Biotechnol 84:1125–1135

    Article  CAS  PubMed  Google Scholar 

  13. Frostegård A, Bååth E (1996) The use of phospholipid fatty acid analysis to estimate bacterial and fungal biomass in soil. Biol Fertil Soils 22:59–65

    Article  Google Scholar 

  14. Hedrick DB, Peacock A, White DC (2005) Monitoring and assessing soil bioremediation. Interpretation of fatty acid profiles of soil microorganisms. Springer, Berlin, pp 251–259

    Google Scholar 

  15. Ishii K, Fukui M, Takii S (2000) Microbial succession during a composting process as evaluated by denaturing gradient gel electrophoresis analysis. J Appl Microbiol 89:768–777

    Article  CAS  PubMed  Google Scholar 

  16. Jpg G (1988) Nutrition and compost. In: van Griensven LJLD (ed) The cultivation of mushrooms. Darlington Mushroom Laboratories, Rustington, pp 29–72

    Google Scholar 

  17. Kato K, Miura N, Tabuchi H, Nioh I (2005) Evaluation of maturity of poultry manure compost by phospholipid fatty acids analysis. Biol Fertil Soils 41:399–410

    Article  Google Scholar 

  18. Klamer M, Bååth E (1998) Microbial community dynamics during composting of straw material studied using phospholipid fatty acid analysis. FEMS Microbiol Ecol 27:9–20

    Article  CAS  Google Scholar 

  19. LaMontagne MG, Michel FC Jr, Holden PA, Reddy CA (2002) Evaluation of extraction and purification methods for obtaining PCR-amplifiable DNA from compost for microbial community analysis. J Microbiol Methods 49:255–264

    Article  CAS  PubMed  Google Scholar 

  20. Liu D, Zhang R, Wu H, Xu D, Tang Z, Yu G, Xua Z, Shen Q (2011) Changes in biochemical and microbiological parameters during the period of rapid composting of dairy manure with rice chaff. Bioresour Technol 102:9040–9049

    Article  CAS  PubMed  Google Scholar 

  21. Macdonald LM, Paterson E, Dawson LA, McDonald AJS (2004) Short-term effects of defoliation on the soil microbial community associated with two contrasting Lolium perenne cultivars. Soil Biol Biochem 36:489–498

    Article  CAS  Google Scholar 

  22. Mengel K, Kirkby EA, Kosegarten H, Appel T (2001) Further elements of importance. In: Mengel K, Kirkby EA, Kosegarten H, Appel T (eds) Principles of plant nutrition. Springer, Dordrecht, pp 639–655

    Chapter  Google Scholar 

  23. Muyzer G, De Waal EC, Uitterlinden AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59:695–700

    CAS  PubMed  PubMed Central  Google Scholar 

  24. Nübel U, Garcia-Pichel F, Kühl M, Muyzer G (1999) Quantifying microbial diversity: morphotypes, 16s rRNA genes, and carotenoids of oxygenic phototrophs in microbial mats. Appl Environ Microbiol 65:422–430

    PubMed  PubMed Central  Google Scholar 

  25. Noble R, Gaze R (1994) Controlled environment composting for mushroom cultivation: substrates based on wheat and barley straw and deep litter poultry manure. J Agric Sci 123:71–79

    Article  Google Scholar 

  26. Noble R, Hobbs JP, Mead A, Dobrovin-Pennington A (2002) Influence of straw types and nitrogen sources on mushroom composting emissions and compost productivity. J Ind Microbiol Biotechnol 29:99–110

    Article  CAS  PubMed  Google Scholar 

  27. Peters S, Koschinsky S, Schwieger F, Tebbe CC (2000) Succession of microbial communities during hot composting as detected by PCR-single-strand-conformation polymorphism-based genetic profiles of small-subunit rRNA genes. Appl Environ Microbiol 66:930–936

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Rashad FM, Saleh WD, Moselhy MA (2010) Bioconversion of rice straw and certain agro-industrial wastes to amendments for organic farming systems: 1. Composting, quality, stability and maturity indices. Bioresour Technol 101:5952–5960

    Article  CAS  PubMed  Google Scholar 

  29. Ross RC, Harris PJ (1982) Some factors involved in phase II of mushroom compost preparation. Sci Hortic 19:223–229

    Article  Google Scholar 

  30. Royse DJ (1985) Effect of spawn run time and substrate nutrition on yield and size of the shiitake mushroom. Mycologia 77:756–762

    Article  Google Scholar 

  31. Scrase R (1996) Cultivating mushrooms: making composted and non-composted substrates. Mycologist 10:52–55

    Article  Google Scholar 

  32. Sekiguchi H, Watanabe M, Nakahara T, Xu B, Uchiyama H (2002) Succession of bacterial community structure along the Changjiang river determined by denaturing gradient gel electrophoresis and clone library analysis. Appl Environ Microbiol 68:5142–5150

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Sharma S, Rangger A, Insam H (1998) Effects of decomposing maize litter on community level physiological profiles of soil bacteria. Microbiol Ecol 35:301–310

    Article  CAS  Google Scholar 

  34. Simsek H, Baysal E, Colak M, Toker H, Yilmaz F (2008) Yield response of mushroom (Agaricus bisporus) on wheat straw and waste tea leaves based composts using supplements of some locally available peats and their mixture with some secondary casing materials. Afr J Biotechnol 7:88–94

    CAS  Google Scholar 

  35. T-t Song, W-m Cai, Q-l Jin, W-l Feng, L-j Fan, Y-y Shen, Fang H, F-f Tian (2014) Comparison of microbial communities and histological changes in phase I rice straw-based Agaricus bisporus compost prepared using two composting methods. Sci Hortic 174:96–104

    Article  Google Scholar 

  36. Straatsma G, Gerrits JPG, Thissen JTNM, Amsing JGM, Loeffen H, Van Griensven LJLD (2000) Adjustment of the composting process for mushroom cultivation based on initial substrate composition. Bioresour Technol 72:67–74

    Article  CAS  Google Scholar 

  37. Szekely AJ, Sipos R, Berta B, Vajna B, Hajdu C, Marialigeti K (2009) DGGE and T-RFLP analysis of bacterial succession during mushroom compost production and sequence-aided T-RFLP profile of mature compost. Microb Ecol 57:522–533

    Article  PubMed  Google Scholar 

  38. Tiquia SM, Wan H, Tam NF (2002) Microbial population dynamics and enzyme activities during composting. Compost Sci Util 10:150–161

    Article  Google Scholar 

  39. Vargas-García M, Suárez-Estrella F, López M, Moreno J (2010) Microbial population dynamics and enzyme activities in composting processes with different starting materials. Waste Manag 30:771–778

    Article  PubMed  Google Scholar 

  40. Wang CM, Shyu CL, Ho SP, Chiou SH (2007) Species diversity and substrate utilization patterns of thermophilic bacterial communities in hot aerobic poultry and cattle manure composts. Microb Ecol 54:1–9

    Article  PubMed  Google Scholar 

  41. Yu M, Zeng G, Chen Y, Yu H, Huang D, Tang L (2009) Influence of Phanerochaete chrysosporium on microbial communities and lignocellulose degradation during solid-state fermentation of rice straw. Process Biochem 44:17–22

    Article  CAS  Google Scholar 

  42. Yu Z, Morrison M (2004) Comparisons of different hypervariable regions of rrs genes for use in fingerprinting of microbial communities by PCR-denaturing gradient gel electrophoresis. Appl Environ Microbiol 70:4800–4806

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  43. Zelles L (1999) Fatty acid patterns of phospholipids and lipopolysaccharides in the characterisation of microbial communities in soil: a review. Biol Fertil Soils 29:111–129

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was financially supported by the Jiangsu Agricultural Science and Technology Independent Innovation Fund Project CX(13)5072.

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Authors and Affiliations

  1. Nanjing Institute of Agricultural Sciences in Jiangsu Hilly Area, Nanjing, 210046, Jiangsu, China

    Lin Wang, Jiugeng Mao, Hejuan Zhao, Qishun Wei, Ying Zhou & Heping Shao

  2. Weifang University of Science and Technology, Shouguang, 262700, Shandong, China

    Min Li

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  1. Lin Wang
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  2. Jiugeng Mao
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  4. Min Li
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Correspondence to Lin Wang.

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Wang, L., Mao, J., Zhao, H. et al. Comparison of characterization and microbial communities in rice straw- and wheat straw-based compost for Agaricus bisporus production. J Ind Microbiol Biotechnol 43, 1249–1260 (2016). https://doi.org/10.1007/s10295-016-1799-6

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  • DOI: https://doi.org/10.1007/s10295-016-1799-6

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