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N-terminal region of human ameloblastin synthetic peptide promotes bone formation

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Abstract

The aim of this study was to examine the effect of 16 amino acids of the N-terminal region of human ameloblastin (16N-AMBN) synthetic peptide, on the proliferation and differentiation of MC3T3-E1 cells and bone regeneration. While 16N-AMBN did not affect the proliferation, it induced mRNA expression of type I collagen, alkaline phosphatase (ALP), bone sialoprotein, and osteocalcin. 16N-AMBN also stimulated ALP activity and promoted mineralized nodule formation. On the other hand, these activities were inhibited by anti-16N-AMBN antibody. Treatment of rat calvarial bone defects with 16N-AMBN resulted in almost complete healing compared to that of the control treatments. These findings suggest that 16N-AMBN may be applicable for regeneration therapy of bone defects.

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Acknowledgments

We thank Shoji Kitagawa, Shinji Iizuka, Guangying Qi, Asako Nakata (Hiroshima University) for technical assistance. This work was supported in part by Grants-in-Aid from the Ministry of Education, Science and Culture of Japan (to T.T. and M.K.).

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Authors and Affiliations

  1. Center of Oral Clinical Examination, Hiroshima University Hospital, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan

    Masae Kitagawa

  2. Department of Oral and Maxillofacial Pathobiology, Institute of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan

    Masae Kitagawa, Toshinori Ando, Ajiravudh Subarnbhesaj, Mutsumi Miyauchi & Takashi Takata

  3. Department of Oral Biology, Institute of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan

    Takashi Uchida

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  1. Masae Kitagawa
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  2. Toshinori Ando
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  3. Ajiravudh Subarnbhesaj
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  4. Takashi Uchida
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  5. Mutsumi Miyauchi
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  6. Takashi Takata
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Correspondence to Takashi Takata.

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Kitagawa, M., Ando, T., Subarnbhesaj, A. et al. N-terminal region of human ameloblastin synthetic peptide promotes bone formation. Odontology 105, 116–121 (2017). https://doi.org/10.1007/s10266-016-0243-8

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  • DOI: https://doi.org/10.1007/s10266-016-0243-8

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