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Effects of dentifrices differing in fluoride compounds on artificial enamel caries lesions in vitro

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Abstract

The aim of this study was to compare the caries-preventive effect of a stabilized stannous fluoride/sodium fluoride dentifrice containing sodium hexametaphosphate with those of a regular, solely sodium fluoride-containing and amine fluoride-containing dentifrice on pre-demineralized bovine enamel specimens using a pH-cycling model. Bovine enamel specimens with two artificial lesions each were prepared. Baseline mineral loss of both lesions was analyzed using transversal microradiography (TMR). Eighty-five specimens with a mean (SD) baseline mineral loss of 3393 (683) vol% × µm were selected and randomly allocated to five groups (n = 13/15). Treatments during pH-cycling (28 days and 2 × 20 min demineralization/day) were: brushing twice daily with slurries of AmF (1400 ppm F−), NaF (1450 ppm F−), SnF2/NaF (1100 ppm F−/350 ppm F−), and fluoride-free (FF) dentifrices or they were immersed in distilled water and remained unbrushed (NB). Subsequently, from each specimen one lesion was covered with acid-resistant varnish, while the remaining lesion was demineralized for another 14 days. Differences in integrated mineral loss (∆∆Z) were calculated between values before and after pH-cycling (∆∆Z E1) as well as before pH-cycling and after second demineralization (∆∆Z E2) using TMR. Treatments AmF and NaF induced a significantly higher mineral gain (∆∆Z E1/∆∆Z E2) compared to treatments FF and NB (p < 0.05; ANOVA test). Except for treatments AmF and NaF no significant differences in mineral loss between before and after pH-cycling could be observed (p < 0.05; t test) [∆∆Z E1: AmF:1563 (767); NaF:1222 (1246); SnF2/NaF:258 (1259); FF:−52 (1223); NB:−151 (834)]. Both dentifrices with either AmF or NaF promoted remineralization, whereas SnF2/NaF dentifrice did not promote remineralization in a biofilm-free pH-cycling model.

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Acknowledgments

This study was funded by the authors and their institution.

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    Authors and Affiliations

    1. Department of Operative Dentistry, Periodontology and Preventive Dentistry, RWTH Aachen University, Pauwelsstrasse 30, 52074, Aachen, Germany

      R. J. Wierichs, M. Esteves-Oliveira & H. Meyer-Lueckel

    2. Clinic for Operative Dentistry and Periodontology, School of Dental Medicine, Christian-Albrechts-Universität zu Kiel, Arnold-Heller-Str. 3, 24105, Kiel, Germany

      H. Zelck & C. E. Doerfer

    3. Abteilung Mineralogie, Institut für Geowissenschaften, Universität Kiel, Ohlshausenstrasse 40, 24098, Kiel, Germany

      P. Appel

    4. Department of Operative and Preventive Dentistry, Charité–Universitätsmedizin Berlin, Assmannshauser Str. 4-6, 14197, Berlin, Germany

      S. Paris

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    1. R. J. Wierichs
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    Correspondence to R. J. Wierichs.

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    R. J. Wierichs and H. Zelck contributed equally to the study.

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    Wierichs, R.J., Zelck, H., Doerfer, C.E. et al. Effects of dentifrices differing in fluoride compounds on artificial enamel caries lesions in vitro. Odontology 105, 36–45 (2017). https://doi.org/10.1007/s10266-016-0233-x

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