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Effect of soil salinity on physiological characteristics of functional leaves of cotton plants

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Abstract

This study analyzes the effects of soil salinity on fatty acid composition, antioxidative enzyme activity, lipid peroxidation, and photosynthesis in functional leaves during the flowering and boll-forming stages of two cotton cultivars, namely, CCRI-44 (salt-tolerant) and Sumian 12 (salt-sensitive), grown under different soil salinity conditions. Saturated (C16:0 and C18:0) and unsaturated fatty acid (FA) contents (C18:1), as well as superoxide dismutase activity increased, whereas high-unsaturated FA (C18:2 and C18:3) decreased, with the increase in soil salinity. The production of malondialdehyde increased with increasing lipoxygenase (LOX) activity, indicating that LOX catalyzed FA peroxidation under salt stress. Soil salinity had no significant effect on catalase (CAT) and peroxidases (POD) activity in the salt-sensitive cultivar Sumian 12, but significantly increased CAT and POD activities in the salt-tolerant cultivar CCRI-44. Net photosynthesis and stomatal conductance of the cotton cultivars decreased in response to salt stress; however, CCRI-44 showed a smaller reduction in photosynthesis than Sumian 12. The results indicated that stomatal apparatus limited leaf photosynthetic capacity in the salinity-treated plants of both cultivars. The net photosynthetic rate, maximum photochemical efficiency, and photochemical quantum yield of the cotton functional leaves showed positive correlation with double-bond index (DBI). These results suggested that salt stress caused DBI reduction and decreased the photochemical conversion efficiency of solar radiation and, thereby resulting in lower net photosynthetic rates.

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References

  • Akter N, Sobahan MA, Hossain MA, Uraji M, Nakamura Y, Mori IC, Murata Y (2010) The involvement of intracellular glutathione in methyl jasmonate signaling in Arabidopsis guard cells. Biosci Biotechnol Biochem 74:2504–2506

    Article  PubMed  CAS  Google Scholar 

  • Allakhverdiev SI, Kinoshita M, Inaba M, Suzuki I, Murata N (2001) Unsaturated fatty acids in membrane lipids protect the photosynthetic machinery against salt induced damage in Synechococus. Plant Physiol 125:1842–1853

    Article  PubMed  CAS  Google Scholar 

  • Badawi GH, Yamauchi Y, Shimada E, Sasaki R, Kawano N, Tanaka K, Tanaka K (2004) Enhanced tolerance to salt stress and water deficit by overexpressing superoxide dismutase in tobacco (Nicotiana tabacum) chloroplasts. Plant Sci 166:919–928

    Article  Google Scholar 

  • Beers RF Jr, Sizer IW (1952) A spectrophotometric method of measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133–140

    PubMed  CAS  Google Scholar 

  • Bird RP, Draper HH (1984) Comparative studies on different methods of malonaldehyde determination. Method Enzymol 105:299–305

    Article  CAS  Google Scholar 

  • Bowler C, Montagu MV, Inze D (1992) Superoxide dismutase and salt stress tolerance. Annu Rev Plant Phys 43:83–116

    Article  CAS  Google Scholar 

  • Brash AR (1999) Lipoxygenases: occurrence, functions, catalysis, and acquisition of substrate. J Biol Chem 274:23679–23682

    Article  PubMed  CAS  Google Scholar 

  • Brugnoli E, Lauteri M (1991) Effects of salinity on stomatal conductance, photosynthetic capacity and carbon istope discrimination of salt-tolerant (Gossypium hirsutum L.) and salt-sensitive (Phaseolus vulgaris L.) C3 non-halophytes. Plant Physiol 95:628–635

    Article  PubMed  CAS  Google Scholar 

  • de Azevedo Neto AD, Prisco JT, Eneas-Filho J, Abreu CEBd, Gomes-Filho E (2006) Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt-tolerant and salt-sensitive maize genotypes. Environ Exp Bot 56: 87–94

  • Dunn GM, Neales TF (1993) Are the effects of salinity on growth and leaf gas-exchange related. Photosynthetic 29:33–42

    CAS  Google Scholar 

  • Ferguson IB, Watkins CB, Harman JEC (1983) Inhibition by calcium of senescence of detached cucumber cotyledons. Plant Physiol 71:182–186

    Article  PubMed  CAS  Google Scholar 

  • Foster JG, Hess JL (1980) Responses of superoxide dismutase and glutathione reductase activities in cotton leaf tissue exposed to an atmosphere enriched in oxygen. Plant Physiol 66:482–487

    Article  PubMed  CAS  Google Scholar 

  • Garratt LC, Janagoudar BS, Lowe KC, Anthony P, Power JB, Davey MR (2002) Salinity tolerance and antioxidant status in cotton cultures. Free Radical Bio Med 33:502–511

    Article  CAS  Google Scholar 

  • Gosset DR, Millhollon EP, Lucas MC (1994) Antioxidant response to NaCl stress in salt-tolerant and salt-sensitive cultivars of cotton. Crop Sci 34:706–714

    Article  Google Scholar 

  • Gueta-Dahan Y, Yaniv Z, Zilinskas BA, Ben-Hayyim G (1997) Salt and oxidative stress: similar and specific responses and their relation to salt tolerance in Citrus. Planta 203:460–469

    Article  PubMed  CAS  Google Scholar 

  • Han HL, Kang FJ (2001) Experiment and study on effects of moisture coerce on cotton production. Trans CSAE 17:37–40 (in Chinese)

    Google Scholar 

  • Heidari M, Tafazoli E (2005) Effect of sodium chloride on lipoxygenase activity, hydrogen peroxide content and lipid peroxidation rate in the seedlings of three Pistacia rootstocks. J Sci Technol Agric Nat Resour 9(2):41–50

    Google Scholar 

  • Hernandez JA, Almansa MS (2002) Short-term effects of salt stress on antioxidant and leaf water relations of pea leaves. Plant Physiol 115:251–257

    Article  CAS  Google Scholar 

  • Isamah GK (2004) ATPase, peroxidase and lipoxygenase activity during post-harvest deterioration of cassava (Manihot esculenta Crantz) root tubers. Int Biodeterior Biodegrad 54:319–323

    Article  CAS  Google Scholar 

  • Jampeetong A, Brix H (2009) Effects of NaCl salinity on growth, morphology, photosynthesis and proline accumulation of Salvinia natans. Aquat Bot 91:181–186

    Article  CAS  Google Scholar 

  • Lagrimini LM, Rothstein S (1987) Tissue specificity of tobacco peroxidase isozymes and their induction by wounding and tobacco mosaic virus infection. Plant Pysiol 84:422–438

    Google Scholar 

  • Liang Y, Chen Q, Liu Q, Zhang W, Ding R (2003) Exogenous silicon (Si) increases antioxidant enzyme activity and reduce lipid peroxidation in roots of salt-stressed barley (Hordeum vulgare L.). J Plant Physiol 160:1157–1164

    Article  PubMed  CAS  Google Scholar 

  • Liavonchanka A, Feussner I (2006) Lipoxygenases: occurrence, functions and catalysis. J Plant Physiol 163:348–357

    Article  PubMed  CAS  Google Scholar 

  • Luchtefeld M, Drexler H, Schieffer B (2003) 5-Lipoxygenase is involved in the angiotensin II-induced NADPH-oxidase activation. Biochem Biophys Res Commun 308:668–672

    Article  PubMed  CAS  Google Scholar 

  • Luna C, Seffino LG, Arias C, Taleisnik E (2000) Oxidative stress indicators as selection tools for salt tolerance in Chloris gayana. Plant Breeding 119:341–345

    Article  CAS  Google Scholar 

  • Mckersie BD, Leshem YY (1994) Stress and stress coping in cultivated plants. Kluwer Academic Publishes, Dordrecht

    Book  Google Scholar 

  • Meloni DA, Oliva MA, Martinez V, Cambraia J (2003) Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environ Exp Bot 49:69–76

    Article  CAS  Google Scholar 

  • Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410

    Article  PubMed  CAS  Google Scholar 

  • Miyao M, Murata N (1989) The mode of binding of three extrinsic proteins of 33 kDa, 23 kDa and 18 kDa in the photosystemII complex of spinach. Biochimeca et Biophysica Acta 977:315–321

    Article  CAS  Google Scholar 

  • Murata N, Takahashi S, Nishiyama Y (2007) Photoinhabition of photosystemII under environmental stress. BIochimeca et Biophysica Acta 1767:414–421

    Article  CAS  Google Scholar 

  • Pang CH, Wang BS (2008) Oxidative stress and salt tolerance in plants. In: Lüttge U, Beyschlag W, Murata J (eds) Progress in botany, vol 69. Springer, Berlin, pp 231–245

  • Porta H, Rueda-Benitez P, Campos F, Colmenero-Flores JM, Colorado JM, Carmona MJ, Covarrubias AA, Rocha-Sosa M (1999) Analysis of lipoxygenase mRNA accumulation in the common bean (Phaseolus vulgaris L.) during development and stress conditions. Plant Cell Physiol 40:850–858

    Article  PubMed  CAS  Google Scholar 

  • Rajguru SN, Banks SW, Gossett DR, Lucas MC, Fowler TE, Millhollon EP (1999) Antioxidant response to salt stress during fiber development in cotton ovules. J Cotton Sci 3:11–18

    CAS  Google Scholar 

  • Rengasamy P (2006) World salinization with emphasis on Australian. J Exp Bot 57:1017–1023

    Article  PubMed  CAS  Google Scholar 

  • Scalet M, Federice R, Guido MC, Manes F (1995) Peroxidase activity and polyamine changes in response to ozone and simulated acid rain in Aleppo pine needles. Environ Exp Bot 35:417–425

    Article  CAS  Google Scholar 

  • Siedow JN (1991) Plant lipoxygenase: structure and function. Annu Rev Plant Biol 42:145–188

    Article  CAS  Google Scholar 

  • Smirnoff N (1998) Plant resistance to environmental stress. Curr Opin Plant Biol 9:214–219

    CAS  Google Scholar 

  • Sofo A, Dichio B, Xiloyannis C, Masia A (2004) Lipoxygenase activity and proline accumulation in leaves and roots of olive trees in response to drought stress. Physiol Plantarum 121:58–65

    Article  CAS  Google Scholar 

  • Somerville C, Browse J (1991) Plant lipids: metabolism, mutants and membrane. Science 252:80–87

    Article  PubMed  CAS  Google Scholar 

  • Steduto P, Albrizio R, Giorio P, Sorrentino G (2000) Gas-exchange response and stomatol and non-stomatol limitations to carbon assimilation of sunflower under salinity. Environ Exp Bot 44:243–255

    Article  PubMed  Google Scholar 

  • Sultana N, Ikeda T, Itoh R (1999) Effect of NaCl salinity on photosynthesis and dry matter accumulation in developing rice grains. Environ Exp Bot 42:211–220

    Article  CAS  Google Scholar 

  • Tan W, Liu J, Dai T, Jing Q, Cao W, Jiang D (2008) Alterations in photosynthesis and antioxidant enzyme activity in winter wheat subjected to post-anthesis waterlogging. Photosynthetica 46:21–27

    Article  CAS  Google Scholar 

  • Yang Y, Han C, Liu Q, Lin B, Wang JW (2008) Effect of drought and low light on growth and enzymatic antioxidant system of Picea asperata seedlings. Acta Physiol Plant 30:433–440

    Article  CAS  Google Scholar 

  • You SZ, Yang HQ, Zhang L, Shao XJ (2009) Effects of cadmium stress on fatty acid composition and lipid peroxidation of Malus hupehensis. Chin J Appl Ecol 28:2032–2037 (in Chinese)

    Google Scholar 

  • Yu HG, Su WA (1996) Studies on relationship between fatty acids desaturation of PSII membrane and low temperature photoinhibation of cucumber. Acta Biochimica Biophysica Sinica 12:227–233

    CAS  Google Scholar 

  • Zhang XZ (1992) Crop physiological research methods. China Agricultural Press, Beijing, pp 131–207 (in Chinese)

  • Zhang M-P, Zhang C-J, Yu G-H, Jiang Y-Z, Strasser RJ, Yuan Z-Y, Yang X-S, Chen G-X (2010) Changes in chloroplast ultrastructure, fatty acid components of thylakoid membrane and chlorophyll a fluorescence transient in flag leaves of a super-high-yield hybrid rice and its parents during the reproductive stage. J Plant Physiol 167:277–285

    Article  PubMed  CAS  Google Scholar 

  • Zhang GW, Lu HL, Zhang L, Chen BL, Zhou ZG (2011) Salt tolerance evaluation of cotton (Gossypium hirsutum L.) at its germinating and seedling stages and selection of related indices. Chin J Appl Ecol 22:2045–2053 (in Chinese)

    CAS  Google Scholar 

  • Zhu SQ, Yu CM, Liu XY (2007) Changes in unsaturated levels of fatty acids in thylakoid PSII membrane lipids during chilling-induced resistance in rice. J Integr Plant Biol 49:463–471

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National High Technology Research and Development Program of China (863 Program) (Grant No. 2007AA10Z206) and Cotton Research & Development Center (CARS).

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Authors and Affiliations

  1. Key Laboratory of Crop Growth Regulation, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People’s Republic of China

    Lei Zhang, Guowei Zhang, Youhua Wang, Zhiguo Zhou, Yali Meng & Binglin Chen

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  1. Lei Zhang
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  2. Guowei Zhang
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  3. Youhua Wang
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  4. Zhiguo Zhou
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  5. Yali Meng
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Correspondence to Zhiguo Zhou.

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L. Zhang and G. Zhang contributed equally to this paper.

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Zhang, L., Zhang, G., Wang, Y. et al. Effect of soil salinity on physiological characteristics of functional leaves of cotton plants. J Plant Res 126, 293–304 (2013). https://doi.org/10.1007/s10265-012-0533-3

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