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Acquisition of embryogenic competency does not require cell division in carrot somatic cell

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Abstract

Totipotency is the ability of a cell to regenerate the entire organism, even after previous differentiation as a specific cell. When totipotency is coupled with active cell division, it was presumed that cell division is essential for this expression. Here, using the stress-induction system of somatic embryos in carrots, we show that cell division is not essential for the expression of totipotency in somatic/embryonic conversion. Morphological and histochemical analyses showed that the cell did not divide during embryo induction. Inhibitors of cell division did not affect the rate of somatic embryo formation. Our results indicate that the newly acquired trait of differentiation appears without cell division, but does not arise with cell division as a newborn cell.

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Abbreviations

AP:

Aphidicolin

BrdU:

5-Bromo-2-deoxyuridine

DAIP:

4′,6-Diamidino-2-phenylindole

DMSO:

Dimethyl sulfoxide

FdU:

5-Fluoro-2′-deoxyuridine

PP:

Propyzamide

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Acknowledgments

This work was supported in part by the Grants-in-Aid program from the Ministry of Education, Culture, Sports, Science and Technology of Japan (Grant no. 23570045) and by the joint research program “Plant Transgenic Research Design, University of Tsukuba.”

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Author notes

  1. Masashi Asahina

    Present address: Department of Biosciences, Teikyo University, 1-1 Toyosatodai, Utsunomiya, Tochigi, 320-8551, Japan

  2. Motoki Tanaka

    Present address: SDS Biotech K.K., Tsukuba Research and Technology Center, 2-1 Midorihara, Tsukuba, Ibaraki, 300-2646, Japan

Authors and Affiliations

  1. Faculty of Life and Environmental Sciences, Gene Research Center, University of Tsukuba, 1-1-1 Ten-noudai, Tsukuba, Ibaraki, 305-8752, Japan

    Akira Kikuchi, Masashi Asahina, Motoki Tanaka, Shinobu Satoh & Hiroshi Kamada

Authors
  1. Akira Kikuchi
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  2. Masashi Asahina
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  3. Motoki Tanaka
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  4. Shinobu Satoh
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  5. Hiroshi Kamada
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Correspondence to Akira Kikuchi.

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Kikuchi, A., Asahina, M., Tanaka, M. et al. Acquisition of embryogenic competency does not require cell division in carrot somatic cell. J Plant Res 126, 243–250 (2013). https://doi.org/10.1007/s10265-012-0517-3

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  • DOI: https://doi.org/10.1007/s10265-012-0517-3

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