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Helicobacter pylori vacA i region polymorphism but not babA2 status associated to gastric cancer risk in northwestern Iran

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Abstract

Helicobacter pylori-specific genotypes have been strongly associated with an increased risk of gastric cancer (GC). The aim of the present work was to study the associations of H. pylori virulence factors, vacA i region polymorphisms and babA2 status with GC risk in Azerbaijan patients. The DNA extracted from gastric biopsy specimens was used to access the babA2 and vacA genotypes. Overall, babA2 was present in 85.39 % (76/89) of H. pylori strains: 19 out of 24 (79.16 %) strains from GC, 16 out of 17 (94.14 %) strains from peptic ulcer disease (PUD) and 41 out of 48 (85.14 %) strains from chronic gastritis. No significant association was found between babA2 genotype and clinical outcomes (P > 0.05). i1 vacA polymorphism was detected in 46/89 (51.68 %) strains: in 21/24 (87.5 %), 6/17 (35.29 %) and 19/48 (39.58 %) patients with GC, PUD and chronic gastritis, respectively. i2 allele was detected in 43 (48.31 %) out of all 89 strains examined: 3 (14.28 %) of 24 strains from GC, 11 (64.71 %) of 17 from PUD, and 29 (60.42 %) of 48 strains from chronic gastritis. In this study, multiple linear regression analysis confirmed the strong association of i1 allele with GC (partial regression correlation 0.455 ± 0.101; P = 0). Results of multiple logistic regression analysis showed that vacA i1 genotype was significantly associated with GC compared with a control group (gastritis) (odds ratio 13.142, 95 % CI 3.116–55.430; P = 0). Findings from the measurement of H. pylori babA2 and vacA genotypes indicate a strong correlation between the vacA i1 allele and GC risk in the Azerbaijan area of Iran.

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Acknowledgments

The authors would like to express gratitude to Tabriz University, and Department of Endoscopy and Faculty of Medicine, Liver and Gastro Intestinal Diseases Research Center, (LGDRC), Imam Reza Hospital, Tabriz, for their help.

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Authors and Affiliations

  1. Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

    Batool Mottaghi, Reza Safaralizadeh & Morteza Bonyadi

  2. Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran

    Saeid Latifi-Navid

  3. Liver and Gastrointestinal Diseases Research Center, (LGDRC), Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad Hossein Somi

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  1. Batool Mottaghi
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Correspondence to Reza Safaralizadeh.

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Mottaghi, B., Safaralizadeh, R., Bonyadi, M. et al. Helicobacter pylori vacA i region polymorphism but not babA2 status associated to gastric cancer risk in northwestern Iran. Clin Exp Med 16, 57–63 (2016). https://doi.org/10.1007/s10238-014-0327-0

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