Abstract
We showed the cis-1,2-dichloroethylene (cis-1,2-DCE) dechlorination ability of Clostridium species DC-1 in association with other bacteria. Result of denaturing gradient gel electrophoresis showed the dominant band pattern of DC-1 during the degradation time of cis-1,2-DCE and dominance of some other Clostridium species. Experiment with addition of Escherichia coli (E. coli) showed that dechlorinating activity of DC-1 was not inhibited by the presence of E. coli. Pour plate experiment with DC-1 and E. coli revealed that the dominance of Clostridium species caused the decrease of E. coli growth in a bioremediation state. This result suggested the possibility of Clostridium species DC-1 as a degrader of cis-1,2-DCE, in a cis-1,2-DCE contaminated site where an indigenous microbial community is present. Experiment conducting with E. coli suggested that the strain in the contaminated site did not inhibit the degradation of cis-1,2-DCE and during the degradation period, rather some other Clostridium species became dominant and the growth of E. coli would be decreased. This finding could be a very positive approach for implementing the dechlorinating bacteria at aliphatic chlorinated component contaminated sites.
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Bhowmik, A., Ishimura, K., Nakamura, K. et al. Degradation activity of Clostridium species DC-1 in the cis-1,2-dichloroethylene contaminated site in the presence of indigenous microorganisms and Escherichia coli . J Mater Cycles Waste Manag 14, 212–219 (2012). https://doi.org/10.1007/s10163-012-0060-2
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DOI: https://doi.org/10.1007/s10163-012-0060-2