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Mechanisms of Tympanic Membrane and Incus Mobility Loss in Acute Otitis Media Model of Guinea Pig

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Abstract

Acute otitis media (AOM) is a rapid infection of middle ear due to bacterial or viral invasion. The infection commonly leads to negative pressure and purulent effusion in the middle ear. To identify how these changes affect tympanic membrane (TM) mobility or sound transmission through the middle ear, we hypothesize that pressure, effusion, and structural changes of the middle ear are the main mechanisms of conductive hearing loss in AOM. To test the hypothesis, a guinea pig AOM model was created by injection of Streptococcus pneumoniae. Three days post inoculation, vibration of the TM at umbo in response to input sound in the ear canal was measured at three experimental stages: intact, pressure-released, and effusion-drained AOM ears. The vibration of the incus tip was also measured after the effusion was removed. Results demonstrate that displacement of the TM increased mainly at low frequencies when pressure was released. As the effusion was removed, the TM mobility increased further but did not reach the level of the normal ear at low frequencies. This was caused by middle ear structural changes or adhesions on ossicles in AOM. The structural changes also affected movement of the incus at low and high frequencies. The results provide new evidence for understanding the mechanism of conductive hearing loss in AOM.

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Acknowledgments

The authors thank Don Nakmali at Hough Ear Institute for his expert technical assistance. This work was supported by OCAST (HR09-033) and NIH (R01DC006632 and R01DC011585).

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Authors and Affiliations

  1. School of Aerospace and Mechanical Engineering and Bioengineering Center, University of Oklahoma, 865 Asp Avenue, Room 200, Norman, OK 73019, USA

    Xiying Guan & Rong Z. Gan

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  1. Xiying Guan
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  2. Rong Z. Gan
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Correspondence to Rong Z. Gan.

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Guan, X., Gan, R.Z. Mechanisms of Tympanic Membrane and Incus Mobility Loss in Acute Otitis Media Model of Guinea Pig. JARO 14, 295–307 (2013). https://doi.org/10.1007/s10162-013-0379-y

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  • DOI: https://doi.org/10.1007/s10162-013-0379-y

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