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Comparison between smaller ruptured intracranial aneurysm and larger un-ruptured intracranial aneurysm: gene expression profile analysis

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Abstract

As it grows in size, an intracranial aneurysm (IA) is prone to rupture. In this study, we compared two extreme groups of IAs, ruptured IAs (RIAs) smaller than 10 mm and un-ruptured IAs (UIAs) larger than 10 mm, to investigate the genes involved in the facilitation and prevention of IA rupture. The aneurismal walls of 6 smaller saccular RIAs (size smaller than 10 mm), 6 larger saccular UIAs (size larger than 10 mm) and 12 paired control arteries were obtained during surgery. The transcription profiles of these samples were studied by microarray analysis. RT-qPCR was used to confirm the expression of the genes of interest. In addition, functional group analysis of the differentially expressed genes was performed. Between smaller RIAs and larger UIAs, 101 genes and 179 genes were significantly over-expressed, respectively. In addition, functional group analysis demonstrated that the up-regulated genes in smaller RIAs mainly participated in the cellular response to metal ions and inorganic substances, while most of the up-regulated genes in larger UIAs were involved in inflammation and extracellular matrix (ECM) organization. Moreover, compared with control arteries, inflammation was up-regulated and muscle-related biological processes were down-regulated in both smaller RIAs and larger UIAs. The genes involved in the cellular response to metal ions and inorganic substances may facilitate the rupture of IAs. In addition, the healing process, involving inflammation and ECM organization, may protect IAs from rupture.

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Acknowledgments

We thank Dr. Zhe Xu for his assistance in analyzing the data.

Author information

Authors and Affiliations

  1. Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No. 6 Tiantanxili, Beijing, 100050, China

    Hao Li, Wen Wang, Lanbing Yu,  ShuoWang, Yong Cao & Jizong Zhao

  2. China National Clinical Research Center for Neurological Diseases, Beijing, 100050, China

    Hao Li, Wen Wang, Lanbing Yu,  ShuoWang, Yong Cao & Jizong Zhao

  3. Laborotary of Clinical Medicine Research, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100050, China

    Haowen Li

  4. Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China

    Haiyan Yue

  5. Beijing Key Laboratory of Noncoding RNA, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China

    Haiyan Yue

  6. University of Chinese Academy of Sciences, Beijing, 100049, China

    Haiyan Yue

  7. Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215123, China

    Wen Wang

Authors
  1. Hao Li
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  2. Haowen Li
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  3. Haiyan Yue
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  4. Wen Wang
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  5. Lanbing Yu
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  6. ShuoWang
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  7. Yong Cao
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  8. Jizong Zhao
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Corresponding authors

Correspondence to Yong Cao or Jizong Zhao.

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Funding

This research was supported by the cooperative project between The Science and Technology Ministry of China and Canada titled “Research on the genetics and pathogenesis of intracranial aneurysm and arteriovenous malformation” (No. 2010 dfb30850).

Conflicts of interest

The authors declare no potential conflicts of interest.

Ethical approval

This study was approved by the Ethics Committee of the Department of Medicine, Beijing Tiantan Hospital, Capital Medical University.

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Informed consent was obtained from all patients.

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Li, H., Li, H., Yue, H. et al. Comparison between smaller ruptured intracranial aneurysm and larger un-ruptured intracranial aneurysm: gene expression profile analysis. Neurosurg Rev 40, 419–425 (2017). https://doi.org/10.1007/s10143-016-0799-3

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  • DOI: https://doi.org/10.1007/s10143-016-0799-3

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