Abstract
The human gut microbiome plays a crucial role in human health and efforts need to be done for cultivation and characterisation of bacteria with potential health benefits. Here, we isolated a bacterium from a healthy Indian adult faeces and investigated its potential as probiotic. The cultured bacterial strain 17OM39 was identified as Enterococcus faecium by 16S rRNA gene sequencing. The strain 17OM39 exhibited tolerance to acidic pH, showed antimicrobial activity and displayed strong cell surface traits such as hydrophobicity and autoaggregation capacity. The strain was able to tolerate bile salts and showed bile salt hydrolytic (BSH) activity, exopolysaccharide production and adherence to human HT-29 cell line. Importantly, partial haemolytic activity was detected and the strain was susceptible to the human serum. Genomics investigation of strain 17OM39 revealed the presence of diverse genes encoding for proteolytic enzymes, stress response systems and the ability to produce essential amino acids, vitamins and antimicrobial compound Bacteriocin-A. No virulence factors and plasmids were found in this genome of the strain 17OM39. Collectively, these physiological and genomic features of 17OM39 confirm the potential of this strain as a candidate probiotic.
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Acknowledgements
The authors are grateful to Kiran Mahale for genome analysis, and Kunal Yadav, Rakeshkumar Yadav, Aniket Saraf, and Kamlesh Jangid for critically reviewing the manuscript.
Funding
This work was supported by the Department of Biotechnology, Government of India, under Grant for National Centre for Microbial Resources (NCMR) (BT/Coord.II/01/03/2016). Additional support was provided by the Department of Biotechnology, Basaveshwar Engineering College, Bagalkot, under TEQUIP Grant to Bharati S. Meti.
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Ghattargi, V.C., Nimonkar, Y.S., Burse, S.A. et al. Genomic and physiological analyses of an indigenous strain, Enterococcus faecium 17OM39. Funct Integr Genomics 18, 385–399 (2018). https://doi.org/10.1007/s10142-018-0596-x
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DOI: https://doi.org/10.1007/s10142-018-0596-x