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Influence of mosquito genotype on transcriptional response to dengue virus infection

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Abstract

The mosquito Aedes aegypti is the principal vector that transmits dengue virus (DENV) to humans. The primary factors that trigger a susceptible or refractory interaction of A. aegypti with DENV are not well understood. In this study, our aim is to characterize the influence of vector genotype on differential gene expression of susceptible vs. refractory A. aegypti strains to DENV infection. To accomplish that, we identified differential expression of a set of complementary DNAs (cDNAs; n = 9,504) of the D2S3 (susceptible) and Moyo-D (refractory) strains of A. aegypti to DENV serotype 2 (JAM1409) and compared these results to the differential expression of cDNAs in a different susceptible vector genotype (Moyo-S) relative to the same refractory genotype (Moyo-D) identified from our previous study. We observed that, although the number of differentially expressed transcripts (DETs) was similar in both the studies, about ~95 % of the DETs were distinct between Moyo-D/D2S3 vs. Moyo-D/Moyo-S. This suggested that A. aegypti response, to infection of a given genotype of dengue, is largely dependent upon the vector genotype. However, we observed a set of common DETs among the vector strains that were associated with predicted functions such as endocytosis, regulation of autophagy, peroxisome, and lipid metabolism that may be relatively universal in conferring mosquito response to DENV infection.

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Acknowledgments

This research was funded by grant RO1-AI059342 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIAID/NIH), USA.

Ethical standards

This study was performed in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The animal use protocol was approved by the University of Notre Dame Institutional Animal Care and Use Committee (study no. 11-036).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, 46556-5645, USA

    Susanta K. Behura, Consuelo Gomez-Machorro, Becky deBruyn, Diane D. Lovin, Brent W. Harker, Jeanne Romero-Severson, Akio Mori & David W. Severson

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  1. Susanta K. Behura
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  2. Consuelo Gomez-Machorro
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  3. Becky deBruyn
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  4. Diane D. Lovin
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  5. Brent W. Harker
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  6. Jeanne Romero-Severson
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  7. Akio Mori
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  8. David W. Severson
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Corresponding author

Correspondence to David W. Severson.

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Behura, S.K., Gomez-Machorro, C., deBruyn, B. et al. Influence of mosquito genotype on transcriptional response to dengue virus infection. Funct Integr Genomics 14, 581–589 (2014). https://doi.org/10.1007/s10142-014-0376-1

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  • DOI: https://doi.org/10.1007/s10142-014-0376-1

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