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Induction of Haploid Androgenesis in Pacific Oyster by UV Irradiation

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Abstract

Androgenesis, development from paternal but not maternal chromosomes, can be induced in some organisms including fish, but has not been induced previously in mollusk. In this study we investigated the induction of haploid androgenesis in the Pacific oyster by ultraviolet irradiation and observed nuclear behavior in the androgenetic eggs. Irradiation for 90 seconds at a UV intensity of 72 erg/mm2 per second (6480 erg/mm2) was the optimal dose to achieve haploid androgenesis. The fertilization and development rates of D-shaped larvae decreased with increasing exposure time, and the development of the genetically inactivated eggs terminated before reaching the D-shaped stage. Cytologic observations showed that UV irradiation did not affect germinal vesicle breakdown or chromosomal condensation but caused various nuclear behavioral patterns during meiosis and first mitosis: 21.7% of eggs extruded all maternal chromosomes as 2 or 3 polar bodies, and 59.1% of eggs formed one female pronucleus. The maternally derived nucleus did not participate, or partially participated, in the first karyokinesis. The cytologic evidence demonstrates that the male genome is directing development in haploids produced by UV irradiation.

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Acknowledgments

The study was supported by the Ministry of Education, Science, Sports, and Culture of Japan (grant 13876041), and the Japan Society for the Promotion of Science (grant P00335). The authors are indebted to the technical staff of Marine Field Station at Onagawa, Field Science Center, Graduate School of Agricultural Science, Tohoku University, for their help in our experiments.

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Authors and Affiliations

  1. The Key Lab of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China

    Qi Li

  2. Marine Field Station, Field Science Center, Graduate School of Agricultural Science, Tohoku University, Onagawa, Oshika 986-2242, Japan

    Tomoko Hisatsune & Akihiro Kijima

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  1. Qi Li
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  2. Tomoko Hisatsune
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  3. Akihiro Kijima
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Correspondence to Akihiro Kijima.

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Li, Q., Hisatsune, T. & Kijima, A. Induction of Haploid Androgenesis in Pacific Oyster by UV Irradiation. Mar. Biotechnol. 6, 291–297 (2004). https://doi.org/10.1007/s10126-003-0034-4

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  • DOI: https://doi.org/10.1007/s10126-003-0034-4

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