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Effects of low-level laser irradiation on human blood lymphocytes in vitro

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Abstract

Low-level laser irradiation (LLLI) has various effects on cultured human lymphocytes in vitro, but little is known about such effects in whole blood. This study investigated whether LLLI affected lymphocyte count in human whole blood in vitro. A total number of 130 blood samples were collected from apparently healthy adult patients through venipuncture into tubes containing EDTA. Each sample was divided into two equal aliquots to be used as a non-irradiated control sample and an irradiated sample. The irradiated aliquot was subjected to laser wavelengths of 405, 589, and 780 nm with different fluences of 36, 54, 72, and 90 J/cm2, at a fixed irradiance of 30 mW/cm2. A paired student t test was used to compare between non-irradiated and irradiated samples. The lymphocyte counts were measured using a computerized hematology analyzer and showed a significant (P < 0.02) maximum increase (1.6%) at a fluence of 72 J/cm2 when compared with non-irradiated samples. This increase in lymphocyte count upon irradiation was confirmed by flow cytometry. At a wavelength of 589 nm and fluence of 72 J/cm2, irradiation of whole blood samples showed a significant increase in CD45 lymphocytes and natural killer (NK) (CD16, CD56) cells, but no significant changes in CD3 T lymphocytes, T-suppressor (CD3, CD8) cells, T-helper (CD3, CD4) cells, and CD19 B lymphocytes when compared with their non-irradiated counterparts. Our results clearly demonstrate that NK cell count is altered by irradiation, which ultimately affects the whole lymphocyte count significantly.

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References

  1. Mester AF, Mester AR (1988) Scientific background of laser biostimulation. Laser 1:23–26

    Google Scholar 

  2. Bihari I, Mester A (1989) The biostimulative effect of low level laser therapy of long-standing crural ulcers using helium neon laser, helium neon plus infrared lasers, and noncoherent light: preliminary report of a randomized double blind comparative study. Laser Ther 1:97–98

    Google Scholar 

  3. Vladimirov Yu A, Osipov AN, Klebanov GI (2004) Photobiological principles of therapeutic applications of laser radiation. Biochemistry 69:81–90

    CAS  PubMed  Google Scholar 

  4. Siposan DG, Lukacs A (2000) Effect of low-level laser radiation on some rheological factors in human blood: an in vitro study. J Clin Laser Med Surg 18:185–195

    Article  CAS  PubMed  Google Scholar 

  5. Pereira AN, Eduardo C de P, Matson E, Marques MM (2002) Effect of low-power laser irradiation on cell growth and procollagen synthesis of cultured fibroblasts. J Clin Laser Med Surg 31:263–267

  6. Piasecka A, Leyko W, Krajewska E, Bryszewska M (2000) Effect of combined treatment with perindoprilat and low-power red light laser irradiation on human erythrocyte membrane fluidity, membrane potential and acetylcholinesterase activity. Scand J Clin Lab Invest 60:395–402

    Article  CAS  PubMed  Google Scholar 

  7. Stadler I, Evans R, Kolb B, Naim JO, Narayan V, Buehner N, Lanzafame RJ (2000) In vitro effects of low-level laser irradiation at 660 nm on peripheral blood lymphocytes. Lasers Surg Med 27:255–261

    Article  CAS  PubMed  Google Scholar 

  8. Yu HS, Wu CS, Yu CL, Kao YH, Chiou MH (2003) Helium-neon laser irradiation stimulates migration and proliferation in melanocytes and induces repigmentation in segmental-type vitiligo. J Invest Dermatol 120:56–64

    Article  CAS  PubMed  Google Scholar 

  9. Stulin ID, Lukacher GIA, Teplova LP, Borovikova EN (1994) The dynamics of the cerebral circulation during the treatment of schizophrenics with a depressive syndrome by means of the intravenous laser irradiation of the blood. J ZhNevrol Psikhiatr ImS SKorsakova 94:54–56

    CAS  Google Scholar 

  10. Almeida-Lopes L, Rigau J, Zângaro RA, Guiduli-Neto J, Jaeger MMM (2001) Comparison of the low-level therapy effects on cultured human gingival fibroblasts proliferation using different irradiance and same fluence. Lasers Surg Med 29:179–184

    Article  CAS  PubMed  Google Scholar 

  11. Moore P, Ridgway TD, Higbee RG, Howard EW, Lucroy MD (2005) Effect of wavelength on low-intensity laser irradiation- stimulated cell proliferation in vitro. Lasers Surg Med 36:8–12

    Article  PubMed  Google Scholar 

  12. KazemiKhoo N, Babazadeh K, Lajevardi M, Dabaghian FH, Mostafavi E (2014) Application of low-level laser therapy following coronary artery bypass grafting (CABG) surgery. J Lasers Med Sci 5:86–91

    Google Scholar 

  13. Yinghua C, Wenming X (2002) Mechanism analysis on ILIB improving the property of rheology of blood. Laser J 2:030

    Google Scholar 

  14. Zalesskaya GA, Sambor EG (2005) Interaction of low-intensity laser radiation with blood and its components. J Appl Spectrosc 72:242–248

    Article  CAS  Google Scholar 

  15. Dörtbudak O, Haas R, Mallath-Pokorny G (2000) Biostimulation of bone marrow cells with a diode soft laser. Clin Oral Implants Res 11:540–545

    Article  PubMed  Google Scholar 

  16. Zalesskaya GA, Sambor EG, Kuchinskii AV (2006) Effect of intravenous laser irradiation on the molecular structure of blood and blood components. J Appl Spectrosc 73:115–122

    Article  CAS  Google Scholar 

  17. Mi XQ, Chen JY, Cen Y, Liang ZJ, Zhou LW (2004) A comparative study of 632.8 and 532 nm laser irradiation on some rheological factors in human blood in vitro. J Photochem Photobiol B 74:7–12

    Article  CAS  PubMed  Google Scholar 

  18. Musawi MS, Jafar MS, Al-Gailani BT, Ahmed NM, Suhaimi FM, Suardi N (2016) In vitro mean red blood cell volume change induced by diode pump solid state low-level laser of 405nm. Photomed Laser Surg 34:211–214

    Article  PubMed  PubMed Central  Google Scholar 

  19. Donnenberg AD, Donnenberg VS (2008) Understanding clinical flow cytometry. Handb Hum Immunol 2:181–220

    Article  Google Scholar 

  20. Karu TI (2003) Low-power laser therapy. Biomedical photonics handbook

  21. Huang YY, Sharma SK, Carroll J, Hamblin MR (2011) Biphasic dose response in low level light therapy—an update. Dose-Response 9:602–618

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Al Musawi MS, Jaafar MS, Al-Gailani BT, Ahmed NM, Suhaimi FM, Bakhsh M (2016) Erythrocyte sedimentation rate of human blood exposed to low-level laser. Lasers Med Sci 1:1–7

    Google Scholar 

  23. Chaves ME, Araújo AR, Piancastelli AC, Pinotti M (2014) Effects of low-power lighttherapy on wound healing: LASER x LED. An Bras Dermatol 89:616–623

    Article  PubMed  PubMed Central  Google Scholar 

  24. Karu TI (1998) Primary and secondary mechanisms of the action of monochromatic visible and near infrared radiation on cells. Sci. low-power laser Ther. Gordon Breach Sci. Amsterdam

  25. Karu T (1999) Primary and secondary mechanisms of action of visible to near-IR radiation on cells. J Photochem Photobiol B 49:1–17

    Article  CAS  PubMed  Google Scholar 

  26. Karu T (1989) Photobiology of low-power laser effects. Health Phys 56:691–704

    Article  CAS  PubMed  Google Scholar 

  27. AlGhamdi KM, Kumar A, Moussa NA (2012) Low-level laser therapy: a useful technique for enhancing the proliferation of various cultured cells. Lasers Med Sci 27:237–249

    Article  PubMed  Google Scholar 

  28. DiMauro S, Schon EA (2006) The mitochondrial respiratory chain and its disorders. In: Mitochondrial Med. Inf. Heal. Abingdon: 7–26

  29. Wasik M, Gorska E, Modzelewska M, Nowicki K, Jakubczak B, Demkow U (2007) The influence of low-power helium-neon laser irradiation on function of selected peripheral blood cells. J Physiol Pharmacol 58:729–737

    PubMed  Google Scholar 

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Authors and Affiliations

  1. School of Physics, Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia

    Mustafa S. Al Musawi, M. S. Jaafar, Naser M. Ahmed & Nursakinah Suardi

  2. Department of Physiology, College of Medicine, Al-Mustansiriya University, Baghdad, Iraq

    Mustafa S. Al Musawi & B. Al-Gailani

  3. Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200, Kepala Batas P. Pinang, Malaysia

    Fatanah M. Suhaimi

Authors
  1. Mustafa S. Al Musawi
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  2. M. S. Jaafar
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  3. B. Al-Gailani
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  4. Naser M. Ahmed
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  5. Fatanah M. Suhaimi
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  6. Nursakinah Suardi
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Correspondence to Mustafa S. Al Musawi.

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No funding was received for this study.

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The authors declare that they have no conflict of interest.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Al Musawi, M.S., Jaafar, M.S., Al-Gailani, B. et al. Effects of low-level laser irradiation on human blood lymphocytes in vitro. Lasers Med Sci 32, 405–411 (2017). https://doi.org/10.1007/s10103-016-2134-1

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  • DOI: https://doi.org/10.1007/s10103-016-2134-1

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