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Photodynamic effects of zinc phthalocyanines on intracellular amastigotes of Leishmania amazonensis and Leishmania braziliensis

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Abstract

This study investigated the photoactivity of four zinc phthalocyanines (PcZns) on a murine macrophage cell line infected with Leishmania amazonensis or Leishmania braziliensis. Infected and uninfected cells were incubated with PcZns at different concentrations (1–10 μM) for 3 h and then exposed to an LED device in continuous wave mode at 660 nm with a fluency of 50 J/cm2 (25 mV). Enzymatic activity was determined by MTT assay 24 h after light treatment. The results demonstrated that all PcZns exhibited high photoactivity, particularly when used at 10 μM. The photodynamic effects were different for uninfected cells versus parasite-infected cells and among the four PcZns. Uninfected cells were more sensitive to photoactivity than infected cells. Although PcZns photodynamic therapy provided promising results, further studies are necessary to better understand its mechanism of action in the treatment of leishmaniasis.

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Acknowledgments

The authors wish to thank Juliana Ferreira (Lab. Terapia Fotodinâmica—IP&D/UNIVAP) for the LED equipment used in this study and Alene Alder-Rangel for assistance with English language revisions.

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Authors and Affiliations

  1. Laboratório de Síntese Orgânica, Instituto de Pesquisa e Desenvolvimento, Universidade do Vale do Paraíba, 2911 Shishima Hifumi Avenue, 12244-000, São José dos Campos, SP, Brazil

    Emanoel Pedro de Oliveira Silva, Vitória Tonini Porto Ferreira & Milton Beltrame Jr.

  2. Laboratório de Parasitologia e Biotecnologia, Instituto de Pesquisa e Desenvolvimento, Universidade do Vale do Paraíba, 2911 Shishima Hifumi Avenue, 12244-000, São José dos Campos, SP, Brazil

    Josane Mittmann

  3. Laboratório de Imunologia, Instituto de Pesquisa e Desenvolvimento, Universidade do Vale do Paraíba, 2911 Shishima Hifumi Avenue, 12244-000, São José dos Campos, SP, Brazil

    Maria Angélica Gargione Cardoso

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  1. Emanoel Pedro de Oliveira Silva
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Correspondence to Milton Beltrame Jr..

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Silva, E.P.d.O., Mittmann, J., Ferreira, V.T.P. et al. Photodynamic effects of zinc phthalocyanines on intracellular amastigotes of Leishmania amazonensis and Leishmania braziliensis . Lasers Med Sci 30, 347–354 (2015). https://doi.org/10.1007/s10103-014-1665-6

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  • DOI: https://doi.org/10.1007/s10103-014-1665-6

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