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Apoptotic effect of pheophorbide a-mediated photodynamic therapy on DMBA/TPA-induced mouse papillomas

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Abstract

Pheophorbide a (Pa) is a chlorine-based photosensitizer, and Pa-mediated photodynamic therapy (PDT) reportedly exhibits antitumor activity against various malignancies. The aim of our study was to investigate the therapeutic effect of Pa-mediated PDT on 7,12-dimethylbenz[a]anthracene (DMBA)/12-O-tetradecanoylphorobol-13-acetate (TPA)-induced mouse papillomas. Thirty mice received a topical application of DMBA/TPA on their backs to induce mouse papillomas. One week after two sessions of Pa-mediated PDT, immunohistochemical stains and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay were performed to evaluate the apoptotic effects thereof on the papillomas. Among 63 mouse papillomas treated with Pa-mediated PDT, 17.5 % of the lesions were completely removed 1 week after the first treatment, while 31.7 % disappeared 1 week after the second treatment. Statistical analyses revealed significant differences in therapeutic outcomes for the Pa-mediated PDT group in comparison to a solvent-PDT group and a Pa group. Additionally, a marked downregulation of proliferating cell nuclear antigen expression, as well as upregulation of cleaved caspase 3 and cleaved poly(ADP-ribose) polymerase expression, was noted in the Pa-PDT group, compared to the solvent-PDT group and Pa group. TUNEL assay revealed higher apoptotic cell counts in the Pa-PDT group, although the difference was not statistically significant. Our data demonstrated that Pa-mediated PDT is effective in treating DMBA/TPA-induced mouse papillomas.

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Acknowledgments

This study was supported by a grant from the Korean Health Technology R&D Project by the Ministry for Health, Welfare & Family Affairs, Republic of Korea (no. A100490)

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Authors and Affiliations

  1. Department of Pathology, Yanbian University Hospital, Yanji City, Jilin Province, China

    Xianglan Zhang

  2. Oral and Maxillofacial Surgery, Oral Cancer Research Institute, College of Dentistry, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, Korea

    Xianglan Zhang, Ki-Yoel Kim, Jin Kim & In-Ho Cha

  3. Department of Oral and Maxillofacial Surgery, Wonkwang Dental Research Institute, College of Dentistry, Wonkwang University, Iksan-si, Korea

    Eun Joo Choi

  4. Department of Dermatology, Yanbian University Hospital, Yanji City, Jilin Province, China

    Zhenlong Zheng & Lianhua Zhu

  5. Department of Dermatology and Cutaneous Biology Research Institute, Yonsei University College of Medicine, Seoul, Korea

    Zhenlong Zheng & Sung Bin Cho

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  1. Xianglan Zhang
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  2. Eun Joo Choi
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  3. Zhenlong Zheng
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  4. Lianhua Zhu
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  6. Ki-Yoel Kim
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  7. Jin Kim
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  8. In-Ho Cha
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Correspondence to In-Ho Cha.

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Xianglan Zhang and Eun Joo Choi contributed equally to this work.

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Zhang, X., Choi, E.J., Zheng, Z. et al. Apoptotic effect of pheophorbide a-mediated photodynamic therapy on DMBA/TPA-induced mouse papillomas. Lasers Med Sci 30, 51–57 (2015). https://doi.org/10.1007/s10103-014-1615-3

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  • DOI: https://doi.org/10.1007/s10103-014-1615-3

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