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Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells

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Abstract

The study investigated the effects of low-level laser radiation and epidermal growth factor (EGF) on adult adipose-derived stem cells (ADSCs) isolated from human adipose tissue. Isolated cells were cultured to semi-confluence, and the monolayers of ADSCs were exposed to low-level laser at 5 J/cm2 using 636 nm diode laser. Cell viability and proliferation were monitored using adenosine triphosphate (ATP) luminescence and optical density at 0 h, 24 h and 48 h after irradiation. Application of low-level laser irradiation at 5 J/cm2 on human ADSCs cultured with EGF increased the viability and proliferation of these cells. The results indicate that low-level laser irradiation in combination with EGF enhances the proliferation and maintenance of ADSCs in vitro.

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References

  1. Kochar PG (2003) What are stem cells. Overview. CSA, Guide to Discovery

  2. Zuk PA, Zhu M, Ashjian P, Daniel A, Ugarte D, Huang JI, Mizuno H, Alfonso ZC, Fraser JK, Benhaim P, Hedrick MH (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13:4279–4295. doi:10.1091/mbc.E02-02-0105

    Article  CAS  PubMed  Google Scholar 

  3. Moore TJ (2007) Stem Cell Q and A – an introduction to stem cells and their role in scientific and medical research. Med Tech SA 21(1): 3–6

    Google Scholar 

  4. Gimble JM, Kartz AJ, Bunnell BA (2007) Adipose derived stem cells for regenerative medicine. Circ Res 100:1249. doi:10.1161/01.RES.0000265074.83288.09

    Article  CAS  PubMed  Google Scholar 

  5. Zuk PA, Zhu M, Mizuno H, Huang JI, Chaudhari S, Lorenz HP, Benhaim P, Hedrick MH (2001) Multilineage cells derived from human adipose tissue, a putative source of stem cells for tissue engineering. Tissue Eng 7:211–216. doi:10.1089/107632701300062859

    Article  CAS  PubMed  Google Scholar 

  6. Masson NM, Currie IS, Terrace JD, Garden OJ, Parks RW, Ross JA (2006) Hepatic progenitor cells in human fetal liver express the oval cell marker Thy-1. Am J Physiol Gastrointest Liver Physiol 291:G45–G54. doi:10.1152/ajpgi.00465.2005

    Article  CAS  PubMed  Google Scholar 

  7. Moore P, Ridgway TD, Higbee RG, Howard EW, Lucroy MD (2005) Effect of wavelength on low-intensity laser irradiation-stimulated cell proliferation in vitro. Lasers Surg Med 36:8–12. doi:10.1002/lsm.20117

    Article  PubMed  Google Scholar 

  8. Gasparyan L, Brill G, Makela A (2004) Influence of low level laser radiation on migration of stem cells, Laser Florence 2004, pp 1–7

  9. Tuby H, Maltz L, Oron U (2007) Low level laser irradiation (LLLI) promotes proliferation of mesenchymal and cardiac stem cells in culture. Lasers Surg Med 39:373–378. doi:10.1002/lsm.20492

    Article  PubMed  Google Scholar 

  10. Gimble JM (2003) Adipose tissue derived therapeutics. Opin Biol Ther 3:705–713. doi:10.1517/14712598.3.5.705

    Article  CAS  Google Scholar 

  11. Bouis D, Kusumanto YK, Meijer C, Mulder NH, Hospers GAP (2006) A review on pro- and anti-angiogenic factors as targets of clinical intervention. Pharmacol Res 53:89–103. doi:10.1016/j.phrs.2005.10.006

    Article  CAS  PubMed  Google Scholar 

  12. Li H, Fu X, Ouyang Y, Cai CL, Wang J, Sun T (2006) Adult bone-marrow derived mesenchymal stem cells contribute to wound healing of skin appendages. Cell Tissue Res 326:725–736. doi:10.1007/s00441-006-0270-9

    Article  CAS  PubMed  Google Scholar 

  13. Fu XB, Sun XQ, Sun TZ, Dong YH, Gu XM, Chen W, Sheng ZY (2002) Epidermal growth factor stimulates tissue repair in skin through skin stem cell activation. Zhongguo Xiu Fu Chong Jian Wai ke Za Zhi 6:31–35

    Google Scholar 

  14. Svendsen CN, Fawcett JW, Bentlage C, Dunnett SB (1995) Increased survival of rat EGF-generated CNS precursor cells using B27 supplemented medium. Exp Brain Res 102:407–414. doi:10.1007/BF00230645

    Article  CAS  PubMed  Google Scholar 

  15. Safford KM, Hicok KC, Safford SD, Halvorsen YD, Wilkison WO, Gimble JM, Rice HE (2002) Neurogenic differentiation of murine and human adipose derived stromal cells. Biochem Biophys Res Commun 294:371–379. doi:10.1016/S0006-291X(02)00469-2

    Article  CAS  PubMed  Google Scholar 

  16. Angenieux B, Schrorderet DF, Arsenijevic Y (2006) Epidermal growth factor is a neuronal differentiation for retinal stem cells in vitro. Stem Cells 24:696–706. doi:10.1634/stemcells.2005-0190

    Article  CAS  PubMed  Google Scholar 

  17. Kratchmarova I, Blagoev B, Haack-Sorensen M, Kassem M, Mann M (2005) Mechanisms of divergent growth factor effects in mesenchymal stem cell differentiation. Science 308:1472–1477. doi:10.1126/science.1107627

    Article  CAS  PubMed  Google Scholar 

  18. Hauner H, Rohrig K, Petruschke T (1995) Effects of epidermal growth factor (EGF), Platelet-derived growth factor (PDGF) and fibroblast growth factor (FGF) on human adipocyte development and function. Eur J Clin Invest 25:90–96. doi:10.1111/j.1365-2362.1995.tb01532.x

    Article  CAS  PubMed  Google Scholar 

  19. Mvula B, Mathope T, Moore T, Abrahamse H (2008) The effect of low level laser therapy on adult human adipose derived stem cells. Lasers Med Sci 23:277–252

    Google Scholar 

  20. Smith PK, Krohn RJ, Hemanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150:76–85. doi:10.1016/0003-2697(85)90442-7

    Article  CAS  PubMed  Google Scholar 

  21. Wilson K, Walker J (1995) Practical biochemistry, principles and techniques, 4th edn. Cambridge University Press, UK pp 434–438

  22. Promega, 2005, Cell Titer-Glo luminescent cell viability assay. Product information. Fact sheet no. G757rev02

  23. Pinheiro ALB, Nascimento SC, Veira ALB, Brugnera A, Zanin FA, Rolim DDS, Da Silva PS (2002) Effects of low level laser therapy on malignant cells: in vitro study. J Clin Laser Med Surg 20:23–26. doi:10.1089/104454702753474977

    Article  PubMed  Google Scholar 

  24. Rao MS (1999) Multipotent and restricted precursors in the central nervous system. Anat Rec 257:137–148. doi:10.1002/(SICI)1097-0185(19990815)257:4<137::AID-AR7>3.0.CO;2-Q

    Article  CAS  PubMed  Google Scholar 

  25. Gimble JM, Guilak F (2003) Adipose derived adult stem cells: isolation, characterisation, and differentiation potential. Cytotherapy 5:362–369. doi:10.1080/14653240310003026

    Article  PubMed  Google Scholar 

  26. Safford KM, Rice HE (2005) Stem cell therapy for neurologic disorders: therapeutic potential of adipose-derived stem cells. Curr Drug Targets 6:57–62. doi:10.2174/1389450053345028

    Article  CAS  PubMed  Google Scholar 

  27. Stein A, Benayahu D, Maltz L, Oron U (2005) Low level laser irradiation promotes proliferation and differentiation of human osteoblasts in vitro. Photomed Laser Surg 23:161–166. doi:10.1089/pho.2005.23.161

    Article  CAS  PubMed  Google Scholar 

  28. Houreld N, Abrahamse H (2005) Low level laser therapy for diabetic foot wound healing. Diabet Foot J 8:182–193

    Google Scholar 

  29. Hawkins D, Houreld N, Abrahamse H (2005) Low level laser therapy (LLLT) as an effective therapeutic modality for delayed wound healing. Ann N Y Acad Sci 1056:486–493. doi:10.1196/annals.1352.040

    Article  CAS  PubMed  Google Scholar 

  30. Kana JS, Hutschenreiter G, Haina D, Waidelich W (1981) Effect of low power density laser radiation on healing on open skin wound in rats. Arch Surg 116:293–296

    CAS  PubMed  Google Scholar 

  31. Boulton M, Marshall J (1986) He-Ne laser stimulation of human fibroblast proliferation and attachment in vitro. Lasers Life Sci 1:123–134

    Google Scholar 

  32. Van Breugel H, Bar PRD (1992) Power density and exposure time of He-Ne laser irradiation are more important than total energy dose in photobiomodulation of human fibroblasts in vitro. Lasers Surg Med 12:528–537. doi:10.1002/lsm.1900120512

    Article  PubMed  Google Scholar 

  33. Anders JJ, Borke RC, Woolery SK, Van der Merwe WP (1993) Low power laser irradiation alters the rate of regeneration of rat facial nerve. Lasers Surg Med 13:72–82. doi:10.1002/lsm.1900130113

    Article  CAS  PubMed  Google Scholar 

  34. Lam TS, Abergel RP, Meeker CA, Castel JC, Ewyer RM, Uitto J (1986) Laser stimulation of collagen synthesis in human skin fibroblasts culture. Lasers Life Sci 1:61–77

    Google Scholar 

  35. Carpenter G, Cohen S (1990) Epidermal growth factor. J Biol Chem 265:7709–7712

    CAS  PubMed  Google Scholar 

  36. Pitman M, Emery B, Binder M, Wang S, Butzkueven H, Kilpatrick TJ (2004) LIF receptor signaling modulates neural stem cell renewal. Mol Cell Neurosci 27:255–266

    CAS  PubMed  Google Scholar 

  37. Tropepe V, Sibilia M, Ciruna BG, Rossant J, Wagner EF, van der Kooy D (1999) Distinct neural stem cells proliferate in response to EGF and FGF in the developing mouse telencephalon. Dev Biol 208:166–188. doi:10.1006/dbio.1998.9192

    Article  CAS  PubMed  Google Scholar 

  38. Heo JS, Lee JL, Han JH (2005) EGF stimulates proliferation of mouse embryonic stem cells: involvement of calcium ions influx and p44/42 MAPKS. Am J Physiol Cell Physiol 290:C123–C133. doi:10.1152/ajpcell.00142.2005

    Article  PubMed  Google Scholar 

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Acknowledgements

This project was supported by the National Laser Centre of South Africa, National Research Foundation of South Africa, Council for Scientific and Industrial Research of South Africa, and the University of Johannesburg. We would like to acknowledge Prof. Alan Widgerow, who supplied us with the donated lipo-aspirate and adipose tissue from consenting adults.

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  1. Laser Research Group, Faculty of Health Science, University of Johannesburg, Doornfontein, Johannesburg, 2028, South Africa

    B. Mvula, T. J. Moore & H. Abrahamse

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  1. B. Mvula
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  2. T. J. Moore
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  3. H. Abrahamse
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Correspondence to H. Abrahamse.

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Mvula, B., Moore, T.J. & Abrahamse, H. Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells. Lasers Med Sci 25, 33–39 (2010). https://doi.org/10.1007/s10103-008-0636-1

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  • DOI: https://doi.org/10.1007/s10103-008-0636-1

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