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Polyclonal neural cell adhesion molecule antibody prolongs the effective duration time of botulinum toxin in decreasing muscle strength

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Abstract

This study aimed to investigate if the effective duration time of botulinum toxin A (Btx-A) could be prolonged by polyclonal neural cell adhesion molecule antibody (P-NCAM-Ab). 175 male SD rats were randomly divided into three major groups: control group (n = 25), Btx-A group (n = 25), and P-NCAM-Ab groups. P-NCAM-Ab groups were composed of five sub-groups, with 25 rats each in the dose-response study. Muscle strength of rat lower limbs was determined using a survey system. The expressions of muscle-specific receptor tyrosine kinase (MuSK) and neural cell adhesion molecule (NCAM) were determined by real-time polymerase chain reactions (RT-PCR) and western blotting (WB). The muscle strength was significantly decreased by Btx-A in Btx-A/P-NCAM-Ab groups compared with normal control group. Besides, the muscle strength of P-NCAM-Ab group was significantly decreased compared with the Btx-A group. The recovery time of muscle strength in P-NCAM-Ab group was significantly longer compared with Btx-A group. RT-PCR and WB assay showed that PNCAM-Ab delayed the increase of MuSK and NCAM after Btx-A injection. P-NCAM-Ab prolongs the effective duration time of Btx-A in decreasing muscle strength, which could provide a novel enhancement in clinical application.

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Acknowledgments

The Project was supported by research Grants including the Natural Science Foundation of China (81000481), the Shanghai Rising-Star Program (10QA1407500), and the Shanghai Health Bureau of Scientific Research Plan (2009Y070).

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The authors declare that there is no financial or other conflict of interest in relation to this research and its publication.

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    Authors and Affiliations

    1. Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine, Xin-Cun Road 389, Shanghai, 200065, China

      Yan Guo, Lizhen Pan, Wuchao Liu, Yougui Pan, Zhiyu Nie & Lingjing Jin

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    1. Yan Guo
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    2. Lizhen Pan
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    3. Wuchao Liu
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    4. Yougui Pan
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    5. Zhiyu Nie
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    Correspondence to Zhiyu Nie or Lingjing Jin.

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    Y. Guo and L. Pan authors have contributed equally to the manuscript.

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    Guo, Y., Pan, L., Liu, W. et al. Polyclonal neural cell adhesion molecule antibody prolongs the effective duration time of botulinum toxin in decreasing muscle strength. Neurol Sci 36, 2019–2025 (2015). https://doi.org/10.1007/s10072-015-2291-1

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