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Antioxidant activities of lotus leaves (Nelumbo nucifera) and barley leaves (Hordeum vulgare) extracts

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Abstract

This study evaluated the antioxidant activities of lotus leaf extracts by methanol (L/M) and ethanol (L/E), and barley leaf extracts, also by methanol (B/M), and ethanol (B/E). The 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of L/M was higher than that of the other extracts at 250 ppm, with the exception of butylhydroxytoluene (BHT). The reducing powers of the treatments at 250 ppm were in the following order: BHT (2.91)>L/M (2.90)>L/E (2.26)>B/E (2.09)>B/M (2.01). In addition, L/M showed the highest concentration of total phenols, total flavonoids, and strongest superoxide dismutase (SOD)-like activity among the treatments. Thus, lotus and barley leaves extracted by methanol and ethanol that have antioxidant activities may be alternatives to synthetic antioxidants in the food industry.

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References

  1. Wang J, Zhang Q, Zhang Z, Li Z. Antioxidant activity of sulfated polysaccharide fractions extracted from Laminaria japonica. Int. J. Biol. Macromol. 42: 127–132 (2008)

    Article  Google Scholar 

  2. Aruom OI. Nutrition and health aspects of free radicals and antioxidant. Food Chem. Toxicol. 62: 671–683 (1994)

    Google Scholar 

  3. Safer AM, Al-Nughamish AJ. Hepatotoxicity induced by the antioxidant food additive butylated hydroxytoluene (BTH) in rats: An electrn microscopical study. Histol. Histopathol. 14: 391–406 (1999)

    CAS  Google Scholar 

  4. Kanatt SR, Chander R, Sharma A. Antioxidant potential of mint (Mentha spicata L.) in radiation-processed lamb meat. Food Chem. 100: 451–458 (2007)

    Article  CAS  Google Scholar 

  5. Jung CH, Seog HM, Choi IW, Park MW, Cho HY. Antioxidant properties of various solvent extracts from wild ginseng leaves. LWT-Food Sci. Technol. 39: 266–274 (2006)

    Article  CAS  Google Scholar 

  6. Lee YS, Joo EY, Kim NW. Polyphenol contents and physiological activity of the Lespedezabicolor extracts. Korean J. Food Preserv. 13: 616–626 (2006)

    Google Scholar 

  7. Ko BS, Jun DW, Jang JS, Kim JH, Park SM. Effect of Sasa Borealis and white lotus roots and leaves on insulin action and secretion in vivo. Korean J. Food Sci. Technol. 38: 114–120 (2006)

    Google Scholar 

  8. Lee YC, Son JY, Kim KT, Kim SS. Antioxidant activity of solvent extract isolated from barley leaves. J. Korean Soc. Food Sci. Nutr. 7: 332–337 (1994)

    Google Scholar 

  9. Mahnaz B, Bryan L, Spangelom YB, Yoshihide H, Hideaki H, Hideo U, Allan LG. Isolation of a vitamin E analog from a green barley leaf extract that stimylates release of prolactin and growth hormone release of prolactin and growth hormone from rat anterior pityitary cells in vitro. J. Nutr. Biochem. 5: 145–150 (1994)

    Article  Google Scholar 

  10. Janis JD, Joji O, Sadatoshi N, Yoshihide H, Takayuki S. Degradation of organophosphorus pesticides in aqueous extracts of young green barley leaves. J. Sci. Food Agr. 79: 1311–1314 (1999)

    Article  Google Scholar 

  11. Lee KW, Kim YJ, Lee HJ, Lee CY. Cocoa has more phenolis phytochemicals and a higher antioxidant capacity than teas and red wine. J. Agr. Food Chem. 51: 7292–7295 (2003)

    Article  CAS  Google Scholar 

  12. Brand-Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebensm.-Wiss. Technol. 28: 25–30 (1995)

    CAS  Google Scholar 

  13. Oyaizu M. Studies on product of browning reaction prepared from glucosamine. Jpn. J. Nutr. 44: 307–315 (1986)

    CAS  Google Scholar 

  14. Slinkard K, Singleton VL. Total phenol analysis: Automation and comparison with manual methods. Am. J. Enol. Viticult. 28: 49–55 (1977)

    CAS  Google Scholar 

  15. Woisky RG, Salatino A. Analysis of propolis: Some parameters and procedures for chemical quality control. J. Apicult. Res. 37: 99–105 (1998)

    CAS  Google Scholar 

  16. Marklund S, Marklund G. Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47: 468–474 (1975)

    Google Scholar 

  17. SAS Institute, Inc. SAS User’s Guide. Statistical Analysis Systems Institute, Cary, NC, USA (1999)

    Google Scholar 

  18. Khattak KF, Simpson TJ, Ihasnullah. Effect of gamma irradiation on the microbial load, nutrient composition, and free radical scavenging activity of Nelumbo nucifera rhizome. Radiat. Phys. Chem. 78: 206–212 (2009)

    Article  CAS  Google Scholar 

  19. Kim NM, Sung HS, Kim WJ. Effect of solvents and some extraction conditions on antioxidant activity in cinnamon extracts. Korean J. Food Sci. Technol. 25: 204–209 (1993)

    Google Scholar 

  20. Sánchenz-Moreno C. Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci. Technol. Int. 8: 121–137 (2002)

    Google Scholar 

  21. Kuljarachanan T, Devahastin S, Chiewchan N. Evalution of antioxidant compounds in lime residues during drying. Food Chem. 113: 944–949 (2009)

    Article  CAS  Google Scholar 

  22. Ozsoy N, Can A, Yanardag R, Akev N. Antioxidant activity of Smilax excels L. leaf extracts. Food Chem. 110: 571–583 (2008)

    Article  CAS  Google Scholar 

  23. Chua MT, Tung YT, Chang ST. Antioxidant activities of ethanolic extracts from the twigs of Cinnamomum osmophloeum. Bioresource Technol. 99: 1918–1925 (2008)

    Article  CAS  Google Scholar 

  24. Lee SO, Lee HJ, Yu MH, Im HG, Lee IS. Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in UIIung Island. Korean J. Food Sci. Technol. 37: 233–240 (2005)

    Google Scholar 

  25. Yen GC, Duh PD. Antioxidant properties of methanolic extracts from peanut hulls. J. Am. Oil Chem. Soc. 70: 383–386 (1993)

    Article  CAS  Google Scholar 

  26. Lee SY, Shin YJ, Park JH, Kim SM Park CS. An analysis of the gyungokgo’s ingredients and a comparison study on anti-oxidation effects according to the kinds of extract. Korean J. Herbol. 23: 123–136 (2008)

    Google Scholar 

  27. Duh PD, Yen WJ, Du PC, Yen GC. Antioxidant activity of mung bean hull. J. Am. Oil Chem. Soc. 75: 1063–1069 (1997)

    Google Scholar 

  28. Kim SB, Rho SB, Rhyu DY, Kim DW. Effect of Nelumbo nucifera leaves on hylerlipidemic and atherosclerotic bio F1B hamster. Korean J. Pharmacogn. 36: 229–234 (2005)

    Google Scholar 

  29. Chang ST, Wu JH, Wang SY, Kang PL, Yang NS, Shyur LF. Antioxidant activity of extracts from Acacia confuse bark and heartwood. J. Agr. Food Chem. 49: 3420–3424 (2001)

    Article  CAS  Google Scholar 

  30. Decker EA. The role of phenolics, conjugated linoleic acid, carnosine, and pyrroloquinoline quinine as nonessential dietary antioxidants. Nutr. Rev. 53: 49–58 (1995)

    CAS  Google Scholar 

  31. Yang D, Wang Q, Ke L, Jiang J, Ying T. Antioxidant activities of various extracts of lotus (Nelumbo nucifera Gaertn.) rhizome. Asia Pac. J. Clin. Nutr. 16: 158–163 (2007)

    Google Scholar 

  32. Rice-Evans CA, Miller NJ, Pagana G. Structure-antioxidant activity relationship of flavonoids and phenolic acids. Free Radical Bio. Med. 20: 933–956 (1996)

    Article  CAS  Google Scholar 

  33. Song JC. Functional Food. Bomoonkak Press, Seoul, Korea. p. 163 (1995)

    Google Scholar 

  34. Elzaawely AA, Xuan TD, Koyama H, Tawata S. Antioxidant activity and contents of essential oil and phenolic compounds in flowers and seeds of A. zerumbet (Pers.) B. L. Burtt. & R. M. Sm. Food Chem. 104: 1648–1653 (2007)

    Article  CAS  Google Scholar 

  35. Meyer AS, Isaksen A. Application of enzymes as food antioxidants. Trends Food Sci. Tech. 6: 300–304 (1995)

    Article  CAS  Google Scholar 

  36. Kim MH, Kang WW, Lee NH, Kweon DJ, Choi UK. Antioxidant activities of extract with water and ethanol of Perilla frutescens var. acuta kudo leaf. J. Korean Soc. Appl. Biol. Chem. 50: 327–333 (2007)

    CAS  Google Scholar 

  37. Lee KS, Kim MG, Lee KY. Antioxidative activity of ethanol extract from lotus (Nelumbo nucifera) leaf. J. Korean Soc. Food Sci. Nutr. 35: 182–186 (2006)

    Article  CAS  Google Scholar 

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Correspondence to Cheon-Jei Kim.

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Choe, JH., Jang, A., Choi, JH. et al. Antioxidant activities of lotus leaves (Nelumbo nucifera) and barley leaves (Hordeum vulgare) extracts. Food Sci Biotechnol 19, 831–836 (2010). https://doi.org/10.1007/s10068-010-0117-8

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  • DOI: https://doi.org/10.1007/s10068-010-0117-8

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