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Relative permittivity measurement during the thrombus formation process using the dielectric relaxation method for various hematocrit values

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Abstract

The relative permittivity ε′ and the dielectric loss ε″ for various hematocrit values H for static bovine blood condition have been measured using the dielectric relaxation method to detect thrombosis in real time. The suitable measurement frequency f m ranged within 60 kHz to 1 MHz, and the relaxation frequency of red blood cells (RBCs) f rc was observed to be 2 MHz. In the f m, the temporal change of normalized ε′ exhibited a minimum (called as bottom point). The bottom point was observed to be exponentially shortened as H increased. This characteristic of the ε′* minimum is discussed from three viewpoints: during fibrin formation, direct thrombus formation, and rouleaux formation processes. ε′* during the fibrin formation process decreased over time, irrespective of f. However, ε′* in f m during the direct thrombus formation process and during the aggregation formation process increased immediately and rapidly over time. Therefore, the ε′* bottom point in f m might be the indication of micrometer-scale thrombus formation by RBC aggregation due to fibrin formation.

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References

  1. Yamane T. The present and future state of non-pulsatile artificial heart technology. J Artif Organ. 2000;5:149–55.

    Article  Google Scholar 

  2. Tamagawa M, Kaneda H, Hiramoto M, Nagahama S. Simulation of thrombus formation in shear flows using Lattice Boltzman method. Artif Organs. 2009;33:604–10.

    Article  PubMed  Google Scholar 

  3. Tamakawa M (2010) Visualization of thrombus formation process on pipe orifice flows by laser sheet. ASME 2010 International Mechanical Engineering Congress and Exposition. doi: 10.1115/IMECE2010-38607.

  4. Oshima S, Sankai Y. Improvement of the accuracy in the optical hematocrit measurement by optimizing mean optical path length. Artif Organs. 2009;33:749–56.

    Article  PubMed  Google Scholar 

  5. Noshiro M, Nebuya S, Fujimaki A, Smallwood R, Brown HB. Frequency characteristics of the electrical conductivity in normal and coagulated blood. Int Fed Med Biol Eng Proc. 2007;17:70–2.

    Google Scholar 

  6. Affani A, Specogna R, Trevisan F (2012) Electrical impedance spectroscopy on flowing blood to predict white thrombus formation in artificial micro-channels. International Instrumentation and Measurement Technology Conference Proceedings. pp, 1477–1480.

  7. Hayashi Y, Katsumoto Y, Omori S, Yasuda A, Asami K, Kaibara M, Uchimura I. Dielectric coagulometry: a new approach to estimate venous thrombosis risk. Anal Chem. 2010;82:9759–74.

    Article  Google Scholar 

  8. Asakura Y, Sapkota A, Maruyama O, Kosaka R, Yamane T, Takei M. Spatio-temporal measurement of sedimenting thrombus in static blood using process tomography. Trans Visual Soc Jpn. 2013;33:17–24 (in Japanese).

    Article  Google Scholar 

  9. Wolf M, Gulich R, Lunkenheimer P, Loidl A. Broadband dielectric spectroscopy on human blood. Biochim Biophys Acta. 2011;1810:727–40.

    Article  CAS  PubMed  Google Scholar 

  10. Asami K. Characterization of biological cells by dielectric spectroscopy. Journal of Non-Crystalline Solids. 2002;305:268–77.

    Article  CAS  Google Scholar 

  11. Baskurt OK. Time course of electrical impedance during red blood cell aggregation in a glass tube: comparison with light transmittance. IEEE Trans Biomed Eng. 2010;57:969–78.

    Article  PubMed  Google Scholar 

  12. Park HJ, Kim SC, Choi CB, Ham YK. The correlation of the complex dielectric constant and blood glucose at low frequency. Biosens Bioelectron. 2003;19:321–4.

    Article  CAS  PubMed  Google Scholar 

  13. Kaibara M. Rheological behaviors of bovine blood forming artificial rouleaux. Biorheology. 1983;20:583–92.

    CAS  PubMed  Google Scholar 

  14. Asaka K. Dielectric properties of cellulose acetate reverse osmosis membranes in aqueous salt solutions. J Membr Sci. 1990;50:71–84.

    Article  CAS  Google Scholar 

  15. Irimajiri A, Ando M, Matsuoka R, Ichinowatari T, Takeuchi S. Dielectric monitoring of rouleaux formation in human whole blood: a feasibility study. Biochim Biophys Acta. 1996;1290:207–9.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported by the Yoshimi T.M.P Grant in 2012 of the Japanese Society for Artificial Organs, the Venture Business Laboratory and the COE Start-up program (Chiba University), Grant-in-Aid for Scientific Research for Young Researchers (26750143), Grant-in-Aid for Challenging Exploratory Research (26630046), and a research grant from Asahi Glass Foundation, Japan. The authors would like to thank Mr. Takuto Fuse (Chiba University, Division of Artificial Systems Science) for his generous help in conducting the experiments and constructive feedback during manuscript preparation and revision.

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Authors and Affiliations

  1. Division of Artificial Systems Science, Graduate Course of Mechanical Engineering, Chiba University, 1-33, Inage, Chiba, 263-8522, Japan

    Yuta Asakura & Masahiro Takei

  2. Department of Information and Computer Engineering, National Institute of Technology, Kisarazu College, 2-22-1 Kiyomidai-Higashi, Kisarazu, Chiba, 292-0041, Japan

    Achyut Sapkota

  3. National Institute of Advanced Industrial Science and Technology, 1-2-1, Namiki, Tsukuba, Ibaraki, 305-8564, Japan

    Osamu Maruyama & Ryo Kosaka

  4. Department of Mechanical Engineering, Graduate School of Engineering, Kobe University, 1-1, Rokkodai, Nada, Kobe, 657-8501, Japan

    Takashi Yamane

Authors
  1. Yuta Asakura
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  2. Achyut Sapkota
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  3. Osamu Maruyama
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  4. Ryo Kosaka
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  5. Takashi Yamane
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  6. Masahiro Takei
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Correspondence to Achyut Sapkota.

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Asakura, Y., Sapkota, A., Maruyama, O. et al. Relative permittivity measurement during the thrombus formation process using the dielectric relaxation method for various hematocrit values. J Artif Organs 18, 346–353 (2015). https://doi.org/10.1007/s10047-015-0847-8

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  • DOI: https://doi.org/10.1007/s10047-015-0847-8

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