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Three-dimensional motion-picture imaging of dynamic object by parallel-phase-shifting digital holographic microscopy using an inverted magnification optical system

  • Special Section: Regular Paper
  • Biomedical Imaging and Sensing Conference (BISC2016), Yokohama, Japan
  • Published:
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Abstract

We constructed a parallel-phase-shifting digital holographic microscopy (PPSDHM) system using an inverted magnification optical system, and succeeded in three-dimensional (3D) motion-picture imaging for 3D displacement of a microscopic object. In the PPSDHM system, the inverted and afocal magnification optical system consisted of a microscope objective (16.56 mm focal length and 0.25 numerical aperture) and a convex lens (300 mm focal length and 82 mm aperture diameter). A polarization-imaging camera was used to record multiple phase-shifted holograms with a single-shot exposure. We recorded an alum crystal, sinking down in aqueous solution of alum, by the constructed PPSDHM system at 60 frames/s for about 20 s and reconstructed high-quality 3D motion-picture image of the crystal. Then, we calculated amounts of displacement of the crystal from the amounts in the focus plane and the magnifications of the magnification optical system, and obtained the 3D trajectory of the crystal by that amounts.

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Acknowledgments

This study was partially supported by Japan Society for the Promotion of Science (JSPS) KAKENHI Grant-in-Aid for Challenging Exploratory Research.

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Authors and Affiliations

  1. Department of Electronics, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki Goshokaido-cho, Sakyo-Ku, Kyoto, 606-8585, Japan

    Takahito Fukuda & Masato Shinomura

  2. Research Institute for Measurement and Analytical Instrumentation, National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, 305-8568, Japan

    Peng Xia

  3. Faculty of Electrical Engineering and Electronics, Kyoto Institute of Technology, Matsugasaki Goshokaido-cho, Sakyo-Ku, Kyoto, 606-8585, Japan

    Yasuhiro Awatsuji

  4. Advanced Technology Center, Kyoto Institute of Technology, Matsugasaki Goshokaido-cho, Sakyo-Ku, Kyoto, 606-8585, Japan

    Kenzo Nishio

  5. Department of Systems Science, Graduate School of System Informatics, Kobe University, Rokkodai 1-1, Nada, Kobe, 657-8501, Japan

    Osamu Matoba

Authors
  1. Takahito Fukuda
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  2. Masato Shinomura
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  3. Peng Xia
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  4. Yasuhiro Awatsuji
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  5. Kenzo Nishio
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  6. Osamu Matoba
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Correspondence to Yasuhiro Awatsuji.

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Fukuda, T., Shinomura, M., Xia, P. et al. Three-dimensional motion-picture imaging of dynamic object by parallel-phase-shifting digital holographic microscopy using an inverted magnification optical system. Opt Rev 24, 206–211 (2017). https://doi.org/10.1007/s10043-016-0279-6

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  • DOI: https://doi.org/10.1007/s10043-016-0279-6

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