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Fabrication of an electrochemical immunosensor for α-fetoprotein based on a poly-L-lysine-single-walled carbon nanotubes/Prussian blue composite film interface

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Abstract

Single-walled carbon nanotubes functionalized with poly-L-lysine (PLL-SWCNTs) were successfully prepared and were used as a biocompatible platform to immobilize α-fetoprotein antibody (anti-AFP) which was labeled with horseradish peroxidase (HRP). Then, anti-AFP-HRP/PLL-SWCNT nanocomposites were coated onto a Prussian blue (PB) film-modified glassy carbon electrode surface. Glutaraldehyde was used to further stabilize the biosensing interface through a cross-linking step. All unspecific sites were blocked by bovine serum albumin to fabricate a novel electrochemical immunosensor for α-fetoprotein determination. The immunosensor was characterized by voltammetry and electrochemical impedance spectroscopy. Based on the catalytic current response of H2O2, the experimental conditions for α-fetoprotein determination were optimized. Under optimal conditions, the current response was linearly related to α-fetoprotein concentration in the range of 0.05∼10.0 and 10.0∼50.0 ng/mL with a detection limit of 0.011 ng/mL. The immunosensor was successfully used for the determination of α-fetoprotein in human blood plasma. The results were satisfied with that obtained with ELISA, demonstrating a good accuracy of the immunosensor.

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Acknowledgments

The authors gratefully acknowledge the financial supports from National Natural Science Foundation of China (no. 21275166), Natural Science Foundation of Hubei Province, China (no. 2015CFA092), and China Scholarship Council (no. 201307780006).

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Authors and Affiliations

  1. Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan, 430074, China

    Yanying Wang, Ying Qu, XiaoXue Ye & Chunya Li

  2. Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, 430074, China

    Yanying Wang & Kangbing Wu

Authors
  1. Yanying Wang
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  2. Ying Qu
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  3. XiaoXue Ye
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  4. Kangbing Wu
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  5. Chunya Li
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Corresponding author

Correspondence to Chunya Li.

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Wang, Y., Qu, Y., Ye, X. et al. Fabrication of an electrochemical immunosensor for α-fetoprotein based on a poly-L-lysine-single-walled carbon nanotubes/Prussian blue composite film interface. J Solid State Electrochem 20, 2217–2222 (2016). https://doi.org/10.1007/s10008-016-3229-0

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  • DOI: https://doi.org/10.1007/s10008-016-3229-0

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