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A sensor for glycoproteins based on dendritic gold nanoparticles electrodeposited on a gold electrode and modified with a phenylboronic acid

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Abstract

We describe a gold electrode capable of recognizing glycoproteins (as exemplified for the model protein horseradish peroxidase; HRP). It is based on the interaction between the cis-diol groups of the glycoprotein and the boronate groups of 4-mercaptophenylboronic acid (MPBA) that was self-assembled on dendritic gold nanoparticles electrodeposition on the surface of a gold electrode. The modified electrode exhibits linear electrochemical response to HRP in the 2.5 nM to 25 μM concentration range. The detection limit is 0.5 nM at a signal-to-noise ratio of 3.

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Acknowledgments

This work was supported by the Natural Science Foundation of Anhui Province (grant no. 1408085 MB36) and Innovative Fund of Anhui Normal University (2012cxjj01)

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Authors and Affiliations

  1. College of Chemistry and Materials Science, Anhui Key Laboratory of Chemo-Biosening, Anhui Normal University, Wuhu, 241000, People’s Republic of China

    Dongdong Shen, Yunchun Liu, Ying Fang, Peng Li & Zhousheng Yang

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  1. Dongdong Shen
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  2. Yunchun Liu
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  3. Ying Fang
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  4. Peng Li
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  5. Zhousheng Yang
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Corresponding author

Correspondence to Zhousheng Yang.

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Fig. A1

The effect of ionic strength on the response. (DOC 85 kb)

Fig. A2

The effect of the deposition time of gold on the steady-state response current of MPBA/ dendritic gold nanoparticles-modified gold electrode in 0.1 M phosphate buffered solution (pH 8.50, NaCl 86 mM). (DOC 94 kb)

Fig. A3

The time of the dendritic gold nanoparticles-modified gold electrode modification with MPBA influences on the d the linear range. (DOC 82 kb)

Fig. A4

The pH of buffer influences on the linear range. (DOC 87 kb)

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Shen, D., Liu, Y., Fang, Y. et al. A sensor for glycoproteins based on dendritic gold nanoparticles electrodeposited on a gold electrode and modified with a phenylboronic acid. J Solid State Electrochem 19, 563–568 (2015). https://doi.org/10.1007/s10008-014-2636-3

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  • DOI: https://doi.org/10.1007/s10008-014-2636-3

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